L3HYPDH (NM_144581) Human Tagged ORF Clone

CAT#: RC204205

L3HYPDH (Myc-DDK-tagged)-Human chromosome 14 open reading frame 149 (C14orf149)

Product Images

Circular map for RC204205

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  Western blot validation of overexpression lysate (Cat# LY408289) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204205 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified L3HYPDH protein (Cat# TP304205). The protein was produced from HEK293T cells transfected with L3HYPDH cDNA clone (Cat# RC204205) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L3HYPDH
• Synonyms: C14orf149
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204205 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGCGCGCTGGCGGTGCCCTGGCTGCCCCCGCATGATCCAGGGACGCCGGTGCTGTCGGTGGTGG
  ACATGCACACGGGCGGCGAGCCCTTGCGTATCGTGCTGGCGGGGTGTCCGGAGGTGTCTGGGCCCACCCT
  GCTGGCCAAGCGGCGCTACATGCGCCAGCACCTTGACCACGTGCGGCGACGGCTCATGTTCGAGCCCCGA
  GGGCACCGGGACATGTACGGGGCGGTCCTAGTCCCGAGCGAGCTGCCGGACGCGCATCTGGGCGTCCTGT
  TCCTGCACAACGAGGGCTACAGCTCCATGTGCGGCCACGCAGTGCTGGCGCTGGGCCGCTTCGCTTTGGA
  CTTCGGGCTTGTGCCGGCGCCCCCTGCGGGCACCCGCGAGGCCCGCGTCAATATCCACTGCCCCTGCGGG
  CTGGTGACCGCCTTCGTGGCATGCGAGGACGGCCGCAGCCACGGACCGGTGCGCTTCCACAGCGTCCCGG
  CCTTCGTGCTGGCCACAGATCTCATGGTGGATGTTCCTGGACATGGAAAGGTGATGGTGGACATTGCATA
  TGGCGGTGCATTTTATGCATTTGTTACTGCTGAAAAGTTAGGACTAGACATTTGTTCTGCAAAGACCAGG
  GACCTTGTGGATGCAGCGAGTGCAGTGACAGAGGCAGTGAAAGCTCAGTTTAAAATTAATCATCCTGATA
  GTGAAGACCTTGCCTTTTTATATGGAACTATATTAACAGATGGAAAAGATGCTTATACCAAGGAACCAAC
  CACCAACATTTGTGTTTTTGCAGATGAACAGGTTGACAGAAGTCCCACTGGCTCAGGAGTGACAGCCCGA
  ATTGCCTTACAGTATCACAAAGGGCTTCTGGAACTGAACCAGATGAGAGCCTTCAAAAGCAGTGCAACTG
  GCTCAGTATTCACAGGGAAAGCTGTGAGGGAAGCGAAATGTGGTGATTTTAAAGCTGTTATAGTGGAAGT
  ATCAGGACAAGCCCATTACACGGGTACAGCAAGCTTTATAATAGAAGATGACGACCCATTGAGGGATGGA
  TTTCTTCTCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204205 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MESALAVPWLPPHDPGTPVLSVVDMHTGGEPLRIVLAGCPEVSGPTLLAKRRYMRQHLDHVRRRLMFEPR
  GHRDMYGAVLVPSELPDAHLGVLFLHNEGYSSMCGHAVLALGRFALDFGLVPAPPAGTREARVNIHCPCG
  LVTAFVACEDGRSHGPVRFHSVPAFVLATDLMVDVPGHGKVMVDIAYGGAFYAFVTAEKLGLDICSAKTR
  DLVDAASAVTEAVKAQFKINHPDSEDLAFLYGTILTDGKDAYTKEPTTNICVFADEQVDRSPTGSGVTAR
  IALQYHKGLLELNQMRAFKSSATGSVFTGKAVREAKCGDFKAVIVEVSGQAHYTGTASFIIEDDDPLRDG
  FLLK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144581
• ORF Size: 1062 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144581.1, NP_653182.1
• RefSeq Size: 1381 bp
• RefSeq ORF: 1065 bp
• Locus ID: 112849
• UniProt ID: Q96EM0
• Cytogenetics: 14q23.1
• MW: 38.2 kDa
• Gene Summary: The protein encoded by this gene is a dehydratase that converts trans-3-hydroxy-L-proline to delta(1)-pyrroline-2-carboxylate. This enzyme may function to degrade dietary proteins that contain trans-3-hydroxy-L-proline as well as other proteins such as collagen IV. The encoded protein can be converted to an epimerase by changing a threonine to a cysteine at a catalytic site. [provided by RefSeq, Sep 2016]