RGS14 (NM_006480) Human Tagged ORF Clone

CAT#: RC204139

RGS14 (Myc-DDK-tagged)-Human regulator of G-protein signaling 14 (RGS14)

Product Images

Circular map for RC204139

• 

  Western blot validation of overexpression lysate (Cat# LY416615) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204139 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified RGS14 protein (Cat# TP304139). The protein was produced from HEK293T cells transfected with RGS14 cDNA clone (Cat# RC204139) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RGS14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204139 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGGGAAGCCCAAGCACCTGGGCGTCCCCAACGGGCGCATGGTTCTGGCTGTGTCAGATGGAGAGC
  TGAGCAGCACGACGGGGCCCCAGGGCCAGGGCGAGGGCCGCGGCAGCTCTCTCAGCATCCACAGCCTCCC
  CAGTGGTCCCAGCAGCCCCTTCCCAACCGAGGAGCAGCCTGTGGCCAGCTGGGCCCTGTCCTTCGAGCGG
  CTGTTGCAGGACCCGCTGGGCCTGGCTTACTTCACTGAGTTCCTGAAGAAGGAGTTCAGCGCGGAAAACG
  TGACTTTCTGGAAGGCCTGCGAGCGCTTCCAGCAGATCCCGGCCAGCGATACCCAGCAGCTAGCTCAGGA
  GGCCCGCAACATCTACCAGGAGTTCCTGTCCAGCCAGGCGCTGAGCCCAGTGAACATCGACCGTCAGGCC
  TGGCTTGGCGAGGAGGTGCTGGCCGAGCCCCGGCCGGACATGTTTCGGGCACAGCAGCTTCAGATCTTCA
  ACTTGATGAAGTTCGACAGCTATGCGCGCTTCGTCAAGTCCCCGCTGTACCGCGAGTGCCTGCTAGCCGA
  AGCCGAGGGACGCCCTCTGCGGGAACCTGGCTCCTCGCGCCTCGGCAGCCCTGACGCCACGAGGAAGAAG
  CCGAAGCTGAAGCCCGGGAAGTCGCTGCCGCTGGGTGTGGAGGAGTTGGGGCAGCTGCCACCCGTTGAGG
  GTCCTGGGGGCCGCCCTCTCCGCAAGTCCTTCCGCCGGGAGCTGGGCGGGACTGCAAACGCCGCCTTGCG
  CCGAGAGTCTCAGGGCTCCCTCAACTCCTCCGCCAGCCTGGACCTTGGCTTCCTAGCCTTCGTCAGCAGC
  AAATCTGAGAGCCACCGGAAGAGCCTTGGGAGCACGGAGGGTGAAAGTGAAAGCCGGCCAGGGAAGTACT
  GCTGTGTGTACCTGCCCGATGGCACAGCCTCCTTGGCCCTGGCCAGACCTGGCCTCACCATCCGAGACAT
  GCTGGCAGGGATCTGTGAGAAACGAGGCCTCTCTCTACCTGACATCAAGGTCTACCTGGTGGGCAATGAA
  CAGGCCCTGGTCCTGGATCAGGACTGCACCGTGCTGGCGGATCAGGAAGTGCGGCTGGAAAACAGGATCA
  CCTTCGAGCTGGAGCTGACGGCGCTGGAGCGCGTGGTACGAATCTCAGCCAAGCCCACCAAGCGGCTGCA
  GGAGGCGCTGCAGCCCATTCTGGAGAAGCACGGCTTGAGCCCGCTAGAGGTGGTGCTGCACCGGCCAGGC
  GAGAAACAGCCTCTGGATCTGGGGAAGCTAGTGAGCTCGGTGGCGGCCCAGAGACTGGTTTTGGACACTC
  TTCCAGGTGTGAAGATCTCCAAAGCCCGTGACAAATCTCCCTGCCGCAGCCAGGGCTGCCCACCTAGAAC
  TCAGGATAAGGCCACCCATCCCCCTCCAGCGTCCCCCAGTTCTCTGGTGAAGGTGCCCAGTAGTGCCACT
  GGAAAGCGGCAGACCTGTGACATCGAAGGCCTGGTGGAGCTGCTGAACCGGGTGCAGAGCAGCGGGGCCC
  ACGACCAGAGGGGCCTTCTGAGGAAAGAGGACCTGGTACTTCCAGAATTTCTGCAGCTGCCCGCCCAAGG
  GCCCAGCTCCGAGGAGACCCCACCACAGACCAAATCAGCAGCCCAGCCCATCGGGGGATCCTTGAACTCC
  ACCACCGACTCAGCCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204139 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPGKPKHLGVPNGRMVLAVSDGELSSTTGPQGQGEGRGSSLSIHSLPSGPSSPFPTEEQPVASWALSFER
  LLQDPLGLAYFTEFLKKEFSAENVTFWKACERFQQIPASDTQQLAQEARNIYQEFLSSQALSPVNIDRQA
  WLGEEVLAEPRPDMFRAQQLQIFNLMKFDSYARFVKSPLYRECLLAEAEGRPLREPGSSRLGSPDATRKK
  PKLKPGKSLPLGVEELGQLPPVEGPGGRPLRKSFRRELGGTANAALRRESQGSLNSSASLDLGFLAFVSS
  KSESHRKSLGSTEGESESRPGKYCCVYLPDGTASLALARPGLTIRDMLAGICEKRGLSLPDIKVYLVGNE
  QALVLDQDCTVLADQEVRLENRITFELELTALERVVRISAKPTKRLQEALQPILEKHGLSPLEVVLHRPG
  EKQPLDLGKLVSSVAAQRLVLDTLPGVKISKARDKSPCRSQGCPPRTQDKATHPPPASPSSLVKVPSSAT
  GKRQTCDIEGLVELLNRVQSSGAHDQRGLLRKEDLVLPEFLQLPAQGPSSEETPPQTKSAAQPIGGSLNS
  TTDSAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006480
• ORF Size: 1698 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006480.5
• RefSeq Size: 2418 bp
• RefSeq ORF: 1701 bp
• Locus ID: 10636
• UniProt ID: O43566
• Cytogenetics: 5q35.3
• Domains: RGS, GoLoco, RBD
• Protein Families: Druggable Genome
• MW: 61.4 kDa
• Gene Summary: This gene encodes a member of the regulator of G-protein signaling family. This protein contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]