GNB1L (NM_053004) Human Tagged ORF Clone

CAT#: RC204086

GNB1L (Myc-DDK-tagged)-Human guanine nucleotide binding protein (G protein), beta polypeptide 1-like (GNB1L)

Product Images

Circular map for RC204086

• 

  Western blot validation of overexpression lysate (Cat# LY409356) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204086 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GNB1L
• Synonyms: DGCRK3; FKSG1; GY2; WDR14; WDVCF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204086 representing NM_053004
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGCCCCCTGCCCGCCGCCACCTCCAGACCCCCAGTTTGTCCTCCGAGGCACCCAGTCACCGGTGC
  ATGCGCTGCACTTCTGCGAAGGAGCCCAGGCTCAGGGGCGCCCGCTCCTCTTCTCAGGGTCTCAGAGTGG
  CCTGGTACACATCTGGAGCCTGCAGACGCGGAGAGCGGTTACCACCCTGGATGGCCACGGCGGCCAGTGT
  GTGACCTGGCTGCAGACGCTGCCCCAGGGGCGCCAGCTCCTCAGTCAGGGCCGGGACCTGAAGCTGTGCC
  TGTGGGACCTCGCGGAGGGCAGGAGCGCTGTCGTGGACTCCGTGTGCTTGGAGAGTGTGGGCTTCTGCCG
  GAGCAGCATCCTGGCCGGGGGCCAGCCACGCTGGACGCTTGCCGTGCCAGGGAGGGGCAGCGACGAGGTT
  CAGATTCTGGAGATGCCCTCCAAGACGTCAGTGTGCGCCCTGAAGCCGAAGGCAGATGCCAAGCTGGGCA
  TGCCCATGTGCCTGCGGCTGTGGCAGGCCGACTGCAGCTCCCGCCCACTCCTTCTGGCCGGCTATGAGGA
  TGGATCGGTGGTCCTGTGGGACGTCTCTGAGCAGAAGGTGTGCAGCCGCATCGCCTGCCATGAGGAGCCC
  GTCATGGACCTTGACTTTGACTCCCAGAAGGCCAGGGGCATCTCAGGCTCCGCGGGGAAGGCGCTGGCTG
  TCTGGAGCCTGGACTGGCAGCAGGCCCTGCAGGTGCGTGGGACTCATGAACTCACCAATCCCGGGATCGC
  CGAGGTCACGATCCGGCCAGATCGCAAGATCCTGGCCACCGCAGGCTGGGACCACCGCATCCGCGTGTTC
  CACTGGCGGACGATGCAGCCACTGGCCGTGCTGGCCTTCCACAGCGCCGCTGTCCAGTGCGTGGCCTTCA
  CCGCCGATGGCTTGCTGGCCGCGGGCTCCAAGGATCAGCGGATCAGCCTCTGGTCACTCTACCCACGCGC
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204086 representing NM_053004
  Red=Cloning site Green=Tags(s)
  
  MTAPCPPPPPDPQFVLRGTQSPVHALHFCEGAQAQGRPLLFSGSQSGLVHIWSLQTRRAVTTLDGHGGQC
  VTWLQTLPQGRQLLSQGRDLKLCLWDLAEGRSAVVDSVCLESVGFCRSSILAGGQPRWTLAVPGRGSDEV
  QILEMPSKTSVCALKPKADAKLGMPMCLRLWQADCSSRPLLLAGYEDGSVVLWDVSEQKVCSRIACHEEP
  VMDLDFDSQKARGISGSAGKALAVWSLDWQQALQVRGTHELTNPGIAEVTIRPDRKILATAGWDHRIRVF
  HWRTMQPLAVLAFHSAAVQCVAFTADGLLAAGSKDQRISLWSLYPRA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053004
• ORF Size: 981 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053004.3
• RefSeq Size: 1537 bp
• RefSeq ORF: 984 bp
• Locus ID: 54584
• UniProt ID: Q9BYB4
• Cytogenetics: 22q11.21
• Domains: WD40
• Protein Families: Druggable Genome
• MW: 35.4 kDa
• Gene Summary: This gene encodes a G-protein beta-subunit-like polypeptide which is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains 6 WD repeats and is highly expressed in the heart. The gene maps to the region on chromosome 22q11, which is deleted in DiGeorge syndrome, trisomic in derivative 22 syndrome and tetrasomic in cat-eye syndrome. Therefore, this gene may contribute to the etiology of those disorders. Transcripts from this gene share exons with some transcripts from the C22orf29 gene. [provided by RefSeq, Jul 2008]