TTC17 (NM_018259) Human Tagged ORF Clone

CAT#: RC204065

TTC17 (Myc-DDK-tagged)-Human tetratricopeptide repeat domain 17 (TTC17)

Product Images

Circular map for RC204065

• 

  Western blot validation of overexpression lysate (Cat# LY413197) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204065 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TTC17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204065 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCAGTAGGGGTTCGTGGCCGGTACGAGCTGCCGCCTTGCTCCGGCCCAGGCTGGCTCCTCA
  GCCTTTCCGCCTTGCTGAGTGTGGCGGCACGAGGGGCCTTCGCCACCACGCACTGGGTCGTCACGGAGGA
  CGGGAAAATCCAGCAGCAGGTGGATTCACCAATGAACTTGAAGCATCCTCATGACCTAGTCATATTAATG
  AGACAAGAAGCAACAGTTAACTACCTCAAAGAATTAGAGAAACAATTAGTTGCTCAAAAAATTCACATAG
  AAGAGAATGAGGACAGAGACACAGGACTGGAACAGAGACATAATAAAGAAGACCCAGACTGCATCAAAGC
  CAAGGTGCCCTTAGGGGACCTGGATCTATATGATGGCACATACATAACTTTGGAGAGCAAAGACATCAGT
  CCTGAAGATTATATAGACACAGAATCTCCTGTCCCTCCAGACCCAGAGCAACCTGATTGTACTAAAATTC
  TAGAACTTCCATATAGTATACATGCTTTTCAGCACTTGAGAGGTGTACAGGAGAGAGTTAATCTTTCTGC
  ACCTCTGCTACCTAAAGAAGACCCAATCTTCACATATTTATCTAAACGGTTAGGAAGGAGTATAGATGAC
  ATAGGTCACCTCATTCATGAAGGCCTACAGAAGAACACTTCCTCGTGGGTACTGTATAACATGGCTTCAT
  TTTACTGGAGAATTAAGAATGAGCCATATCAGGTAGTAGAATGTGCCATGCGAGCACTTCACTTCTCTTC
  CAGGCACAATAAAGACATTGCCCTGGTCAACCTGGCAAACGTTCTACACAGAGCACACTTCTCTGCTGAT
  GCTGCTGTCGTGGTCCATGCAGCTCTGGATGACAGTGACTTCTTCACCAGCTATTACACTTTGGGGAATA
  TATATGCAATGCTTGGGGAATATAACCACTCAGTGCTCTGTTATGACCACGCTTTGCAGGCCAGACCTGG
  GTTTGAGCAAGCTATAAAGAGGAAGCATGCTGTCCTATGTCAGCAAAAACTGGAGCAGAAATTGGAGGCT
  CAGCATAGATCTCTCCAGCGAACACTGAATGAGTTAAAAGAGTATCAAAAGCAGCATGACCACTACCTGA
  GACAGCAGGAAATCCTAGAAAAACATAAACTGATTCAGGAGGAGCAAATCTTAAGAAATATCATTCATGA
  GACTCAGATGGCAAAAGAGGCACAATTAGGAAATCATCAGATATGCCGACTGGTCAACCAGCAGCATAGT
  TTACATTGCCAGTGGGACCAGCCTGTACGCTATCATCGTGGAGATATCTTTGAAAATGTGGACTATGTTC
  AGTTTGGTGAGGATTCATCAACCTCCAGTATGATGTCTGTGAACTTTGATGTTCAATCAAATCAGAGTGA
  TATCAATGATTCGGTCAAGTCTTCTCCCGTAGCCCATTCTATTCTCTGGATTTGGGGCAGGGACTCTGAT
  GCATATAGGGACAAACAGCATATTCTATGGCCTAAAAGAGCAGATTGTACAGAAAGCTACCCTAGAGTCC
  CTGTTGGTGGGGAATTGCCAACGTATTTTCTGCCTCCGGAAAACAAAGGACTCAGGATCCACGAACTCAG
  CAGTGATGATTATTCTACAGAAGAAGAGGCCCAAACCCCTGACTGTTCCATAACTGACTTCAGAAAAAGC
  CACACTCTGTCCTACTTAGTCAAAGAATTAGAGGTTCGCATGGATCTGAAAGCCAAAATGCCAGATGACC
  ATGCACGAAAAATTTTGCTTTCCCGTATTAATAACTATACTATCCCAGAAGAAGAAATTGGGTCTTTCTT
  ATTTCATGCTATTAATAAGCCAAATGCTCCTATCTGGCTCATACTCAATGAAGCTGGACTATACTGGAGA
  GCAGTAGGAAATAGCACTTTTGCTATTGCCTGTCTTCAGAGGGCTTTGAATTTAGCTCCACTTCAATACC
  AAGATGTTCCTCTTGTCAACTTGGCCAACCTTTTGATTCATTACGGCCTTCATCTTGATGCCACTAAGCT
  GCTACTTCAAGCTTTGGCCATCAATAGCTCTGAGCCTCTGACCTTTTTGAGCCTGGGAAATGCTTACCTT
  GCTCTGAAGAATATCAGTGGGGCACTTGAGGCCTTTAGACAGGCCTTGAAATTAACCACCAAATGTCCAG
  AGTGTGAAAACAGCCTGAAGTTGATCCGCTGTATGCAGTTTTATCCTTTTCTGTACAACATCACTTCTTC
  TGTTTGCAGTGGTACGGTGGTTGAGGAGAGCAATGGTTCTGATGAGATGGAGAATTCAGATGAAACCAAA
  ATGTCAGAAGAAATACTGGCTTTGGTGGATGAATTTCAACAGGCATGGCCTTTGGAAGGCTTTGGGGGTG
  CACTAGAGATGAAAGGGCGGCGTCTAGACTTACAAGGAATACGGGTGCTGAAGAAAGGTCCCCAGGATGG
  AGTGGCCAGAAGCTCTTGCTATGGAGACTGCAGAAGTGAAGATGATGAAGCAACAGAATGGATTACATTC
  CAGGTCAAACGTGTAAAGAAACCCAAAGGAGATCATAAGAAAACTCCTGGGAAAAAAGTAGAAACAGGTC
  AGATAGAAAATGGACATCGTTACCAAGCAAACCTAGAGATCACTGGCCCCAAGGTGGCATCTCCTGGGCC
  ACAAGGAAAAAAACGTGACTACCAGCGTCTGGGATGGCCCAGCCCGGACGAATGCCTCAAACTCCGCTGG
  GTAGAGCTGACTGCCATCGTGAGTACCTGGCTTGCAGTTTCTTCAAAAAACATTGACATCACAGAACACA
  TAGATTTTGCCACCCCTATACAGCAGCCAGCAATGGAGCCTCTTTGCAATGGCAATCTCCCCACGAGTAT
  GCATACCCTGGACCACTTGCATGGGGTTTCCAACCGAGCCAGCCTGCACTACACAGGGGAGAGTCAGTTA
  ACAGAGGTATTACAAAATCTCGGCAAAGACCAATATCCACAACAGTCGCTTGAACAGATTGGCACCCGAA
  TTGCCAAAGTTTTGGAAAAGAACCAGACGTCCTGGGTCCTCTCCAGCATGGCAGCCCTCTACTGGAGGGT
  GAAAGGCCAAGGAAAGAAGGCAATCGACTGCCTCCGCCAGGCTCTGCACTATGCGCCACACCAGATGAAG
  GATGTGCCCCTGATTAGCCTGGCCAACATCTTGCACAATGCCAAGCTCTGGAATGACGCCGTCATAGTAG
  CCACCATGGCAGTAGAGATCGCACCACACTTTGCTGTGAACCACTTCACTCTGGGCAATGTCTACGTGGC
  AATGGAAGAATTTGAAAAAGCACTGGTGTGGTATGAATCCACATTGAAGCTTCAGCCCGAGTTTGTCCCA
  GCCAAGAACCGAATCCAGACCATCCAGTGTCACTTAATGCTGAAGAAGGGACGGCGCTCTCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204065 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAAVGVRGRYELPPCSGPGWLLSLSALLSVAARGAFATTHWVVTEDGKIQQQVDSPMNLKHPHDLVILM
  RQEATVNYLKELEKQLVAQKIHIEENEDRDTGLEQRHNKEDPDCIKAKVPLGDLDLYDGTYITLESKDIS
  PEDYIDTESPVPPDPEQPDCTKILELPYSIHAFQHLRGVQERVNLSAPLLPKEDPIFTYLSKRLGRSIDD
  IGHLIHEGLQKNTSSWVLYNMASFYWRIKNEPYQVVECAMRALHFSSRHNKDIALVNLANVLHRAHFSAD
  AAVVVHAALDDSDFFTSYYTLGNIYAMLGEYNHSVLCYDHALQARPGFEQAIKRKHAVLCQQKLEQKLEA
  QHRSLQRTLNELKEYQKQHDHYLRQQEILEKHKLIQEEQILRNIIHETQMAKEAQLGNHQICRLVNQQHS
  LHCQWDQPVRYHRGDIFENVDYVQFGEDSSTSSMMSVNFDVQSNQSDINDSVKSSPVAHSILWIWGRDSD
  AYRDKQHILWPKRADCTESYPRVPVGGELPTYFLPPENKGLRIHELSSDDYSTEEEAQTPDCSITDFRKS
  HTLSYLVKELEVRMDLKAKMPDDHARKILLSRINNYTIPEEEIGSFLFHAINKPNAPIWLILNEAGLYWR
  AVGNSTFAIACLQRALNLAPLQYQDVPLVNLANLLIHYGLHLDATKLLLQALAINSSEPLTFLSLGNAYL
  ALKNISGALEAFRQALKLTTKCPECENSLKLIRCMQFYPFLYNITSSVCSGTVVEESNGSDEMENSDETK
  MSEEILALVDEFQQAWPLEGFGGALEMKGRRLDLQGIRVLKKGPQDGVARSSCYGDCRSEDDEATEWITF
  QVKRVKKPKGDHKKTPGKKVETGQIENGHRYQANLEITGPKVASPGPQGKKRDYQRLGWPSPDECLKLRW
  VELTAIVSTWLAVSSKNIDITEHIDFATPIQQPAMEPLCNGNLPTSMHTLDHLHGVSNRASLHYTGESQL
  TEVLQNLGKDQYPQQSLEQIGTRIAKVLEKNQTSWVLSSMAALYWRVKGQGKKAIDCLRQALHYAPHQMK
  DVPLISLANILHNAKLWNDAVIVATMAVEIAPHFAVNHFTLGNVYVAMEEFEKALVWYESTLKLQPEFVP
  AKNRIQTIQCHLMLKKGRRSP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018259
• ORF Size: 3423 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018259.6
• RefSeq Size: 4525 bp
• RefSeq ORF: 3426 bp
• Locus ID: 55761
• UniProt ID: Q96AE7
• Cytogenetics: 11p12-p11.2
• Domains: TPR
• Protein Families: Protease
• MW: 129.6 kDa
• Gene Summary: Plays a role in primary ciliogenesis by modulating actin polymerization.[UniProtKB/Swiss-Prot Function]