TLE 1 (TLE1) (NM_005077) Human Tagged ORF Clone

CAT#: RC204037

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TLE1 (Myc-DDK-tagged)-Human transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) (TLE1)



  "NM_005077" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 691.00


Availability*
In Stock

Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol TLE1
Synonyms ESG; ESG1; GRG1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC204037 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCCCGCAGAGCCGGCACCCGACGCCGCACCAGGCTGCAGGCCAGCCCTTCAAGTTCACTATCCCGG
AGTCCCTGGACCGGATTAAAGAGGAATTCCAGTTCCTGCAGGCGCAGTATCACAGCCTTAAATTGGAATG
TGAGAAACTGGCAAGTGAAAAGACAGAAATGCAGAGGCACTATGTGATGTATTATGAAATGTCATATGGA
TTAAACATTGAAATGCACAAACAGACTGAAATCGCCAAGAGATTGAATACGATTTGTGCACAAGTCATCC
CATTTCTGTCTCAGGAACATCAACAACAGGTGGCCCAGGCTGTTGAACGTGCCAAACAGGTGACCATGGC
AGAGTTGAATGCCATCATCGGGCAGCAGCAGTTGCAAGCTCAGCATCTTTCTCATGGCCACGGACCCCCA
GTTCCCCTTACGCCTCACCCTTCGGGACTTCAGCCTCCTGGAATCCCGCCCCTCGGGGGCAGTGCCGGCC
TTCTTGCGCTGTCTAGTGCTCTGAGTGGGCAGTCTCACTTGGCAATAAAAGATGACAAGAAGCACCACGA
TGCAGAGCACCACAGAGACAGAGAGCCGGGCACAAGTAATTCCCTCCTGGTCCCAGACAGTCTAAGAGGC
ACAGATAAACGCAGAAATGGACCTGAATTTTCCAATGACATCAAGAAAAGGAAGGTGGATGATAAGGACT
CCAGCCACTATGACAGTGATGGTGACAAAAGCGATGACAACTTAGTTGTGGATGTGTCTAATGAGGACCC
TTCTTCTCCGCGAGCAAGCCCTGCCCACTCGCCCCGGGAAAATGGAATCGACAAAAATCGCCTGCTAAAG
AAGGATGCTTCTAGCAGTCCAGCTTCCACGGCCTCCTCGGCAAGTTCCACTTCTTTGAAATCCAAAGAAA
TGAGCTTGCATGAAAAAGCCAGCACGCCTGTTCTGAAATCCAGCACACCAACGCCTCGGAGCGACATGCC
AACGCCGGGCACCAGCGCCACTCCAGGCCTCCGTCCAGGTCTCGGCAAGCCTCCAGCCATAGACCCCCTC
GTTAACCAAGCGGCAGCTGGCTTGAGGACACCCCTGGCAGTGCCCGGCCCATATCCTGCTCCTTTTGGGA
TGGTCCCCCACGCTGGCATGAACGGCGAGCTGACCAGCCCAGGCGCTGCCTACGCCAGTTTACACAACAT
GTCGCCCCAGATGAGCGCCGCAGCCGCCGCGGCCGCCGTGGTGGCCTACGGGCGCTCCCCCATGGTGGGG
TTTGATCCTCCCCCTCACATGAGAGTACCTACCATTCCTCCAAACCTGGCAGGAATCCCTGGGGGGAAAC
CTGCATACTCCTTCCACGTTACTGCAGACGGTCAGATGCAGCCTGTCCCTTTTCCCCCCGACGCCCTCAT
CGGACCCGGAATCCCCCGGCATGCTCGCCAGATCAACACCCTCAACCACGGGGAGGTGGTGTGCGCTGTG
ACCATCAGCAACCCCACGAGACACGTGTACACAGGCGGGAAGGGCTGCGTCAAGGTCTGGGACATCAGCC
ACCCTGGCAATAAGAGCCCTGTCTCCCAGCTCGACTGTCTGAACAGAGACAATTATATCCGTTCCTGTAA
ATTGCTACCCGATGGCTGCACTCTCATAGTGGGAGGGGAAGCCAGTACTTTGTCCATTTGGGACCTGGCG
GCTCCAACCCCGCGCATCAAGGCGGAGCTGACGTCCTCGGCCCCCGCCTGCTACGCCCTGGCCATCAGCC
CCGATTCCAAGGTCTGCTTCTCATGCTGCAGCGACGGCAACATCGCTGTGTGGGATCTGCACAACCAGAC
ACTAGTGAGGCAATTCCAGGGCCACACAGACGGAGCCAGCTGTATTGACATTTCTAATGATGGCACCAAG
CTCTGGACGGGTGGTTTGGACAACACAGTCAGGTCCTGGGACCTGCGCGAGGGGCGGCAGCTGCAGCAGC
ACGACTTCACCTCCCAGATCTTCTCCCTGGGGTACTGCCCCACCGGGGAGTGGCTGGCAGTGGGCATGGA
GAGCAGCAATGTGGAGGTGCTGCACGTGAACAAGCCTGACAAGTACCAGCTGCACCTGCATGAGAGCTGC
GTGCTGTCCCTGAAATTTGCTTACTGTGGTAAATGGTTTGTGAGTACTGGAAAAGATAACCTCCTCAATG
CTTGGCGGACCCCCTATGGAGCCAGCATATTCCAGTCCAAAGAGTCCTCGTCAGTGCTTAGCTGTGACAT
CTCTGTGGATGATAAGTACATAGTCACTGGCTCGGGGGACAAGAAGGCTACAGTCTATGAAGTCATCTAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC204037 protein sequence
Red=Cloning site Green=Tags(s)

MFPQSRHPTPHQAAGQPFKFTIPESLDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYG
LNIEMHKQTEIAKRLNTICAQVIPFLSQEHQQQVAQAVERAKQVTMAELNAIIGQQQLQAQHLSHGHGPP
VPLTPHPSGLQPPGIPPLGGSAGLLALSSALSGQSHLAIKDDKKHHDAEHHRDREPGTSNSLLVPDSLRG
TDKRRNGPEFSNDIKKRKVDDKDSSHYDSDGDKSDDNLVVDVSNEDPSSPRASPAHSPRENGIDKNRLLK
KDASSSPASTASSASSTSLKSKEMSLHEKASTPVLKSSTPTPRSDMPTPGTSATPGLRPGLGKPPAIDPL
VNQAAAGLRTPLAVPGPYPAPFGMVPHAGMNGELTSPGAAYASLHNMSPQMSAAAAAAAVVAYGRSPMVG
FDPPPHMRVPTIPPNLAGIPGGKPAYSFHVTADGQMQPVPFPPDALIGPGIPRHARQINTLNHGEVVCAV
TISNPTRHVYTGGKGCVKVWDISHPGNKSPVSQLDCLNRDNYIRSCKLLPDGCTLIVGGEASTLSIWDLA
APTPRIKAELTSSAPACYALAISPDSKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISNDGTK
LWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHVNKPDKYQLHLHESC
VLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISVDDKYIVTGSGDKKATVYEVIY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_005077
ORF Size 2310 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005077.3, NP_005068.2
RefSeq Size 3283 bp
RefSeq ORF 2313 bp
Locus ID 7088
UniProt ID Q04724
Cytogenetics 9q21.32
Domains WD40, TLE_N
Protein Families Transcription Factors
MW 83.2 kDa
Gene Summary Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.[UniProtKB/Swiss-Prot Function]

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