BOP1 (NM_015201) Human Tagged ORF Clone

CAT#: RC204016

BOP1 (Myc-DDK-tagged)-Human block of proliferation 1 (BOP1)

Product Images

Circular map for RC204016

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  Western blot validation of overexpression lysate (Cat# LY414723) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204016 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified BOP1 protein (Cat# TP304016). The protein was produced from HEK293T cells transfected with BOP1 cDNA clone (Cat# RC204016) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BOP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204016 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGTTCGCGGGGTGCGGGGCGCACGGCGGCGCCGAGCGTGCGGCCGGAGAAGCGGCGGTCTGAGC
  CCGAACTGGAGCCTGAGCCCGAGCCGGAGCCCCCCCTCCTCTGCACCTCTCCTCTCAGCCACAGCACCGG
  CAGCGATTCTGGCGTCTCCGACAGCGAGGAGAGTGTGTTCTCAGGCCTGGAAGATTCCGGCAGTGACAGC
  AGTGAGGATGATGACGAAGGCGACGAGGAGGGAGAGGACGGAGCCCTTGATGACGAGGGCCACAGTGGGA
  TTAAAAAGACCACTGAGGAGCAGGTGCAGGCCAGCACTCCTTGCCCGAGGACAGAGATGGCGAGCGCCCG
  GATTGGGGATGAGTATGCGGAGGACAGCTCTGATGAGGAGGACATCCGGAACACGGTGGGCAACGTGCCC
  TTGGAGTGGTACGATGACTTCCCCCACGTGGGCTACGACCTGGATGGCAGGCGCATCTACAAGCCCCTGC
  GGACCCGGGATGAGCTGGACCAGTTCCTGGACAAGATGGACGATCCTGACTACTGGCGCACCGTGCAGGA
  CCCGATGACAGGGCGGGACCTGAGACTGACGGATGAGCAGGTGGCCCTGGTGCGGCGGCTGCAGAGTGGC
  CAGTTTGGGGATGTGGGCTTCAACCCCTATGAGCCGGCTGTCGACTTCTTCAGCGGGGACGTCATGATCC
  ACCCGGTGACCAACCGCCCGGCCGACAAGCGCAGCTTCATCCCCTCCCTGGTGGAGAAGGAGAAGGTCTC
  TCGCATGGTGCACGCCATCAAGATGGGCTGGATCCAGCCTCGCCGGCCCCGAGACCCCACCCCCAGCTTC
  TATGACCTGTGGGCCCAGGAGGACCCCAACGCCGTGCTCGGGCGCCACAAGATGCACGTACCTGCTCCCA
  AGCTGGCCCTGCCAGGCCACGCCGAGTCGTACAACCCACCCCCTGAATACCTGCTCAGCGAGGAGGAGCG
  CTTGGCGTGGGAACAGCAGGAGCCAGGCGAGAGGAAGCTGAGCTTTTTGCCACGCAAGTTCCCGAGCCTG
  CGGGCCGTGCCTGCCTACGGACGCTTCATCCAGGAACGCTTCGAGCGCTGCCTTGACCTGTACCTGTGCC
  CACGGCAGCGCAAGATGAGGGTGAATGTAGACCCTGAGGACCTCATCCCCAAGCTGCCTCGGCCGAGGGA
  CCTGCAGCCCTTCCCCACGTGCCAGGCCCTGGTCTACAGGGGCCACAGTGACCTTGTCCGGTGCCTCAGT
  GTCTCTCCTGGGGGCCAGTGGCTGGTTTCAGGCTCTGACGACGGCTCCCTGCGGCTCTGGGAGGTGGCCA
  CTGCCCGCTGTGTGAGGACTGTTCCCGTGGGGGGCGTGGTGAAGAGTGTGGCCTGGAACCCCAGCCCCGC
  TGTCTGCCTGGTGGCTGCAGCCGTGGAGGACTCGGTGCTGCTGCTGAACCCAGCTCTGGGGGACCGGCTG
  GTGGCGGGCAGCACAGATCAGCTGTTGAGCGCCTTCGTCCCGCCTGAGGAGCCCCCCTTGCAGCCGGCCC
  GCTGGCTGGAGGCCTCAGAGGAGGAGCGCCAAGTGGGCCTGCGGCTGCGCATCTGCCACGGGAAGCCAGT
  GACGCAGGTGACCTGGCACGGGCGTGGGGACTACCTGGCCGTGGTGCTGGCCACCCAAGGCCACACCCAG
  GTGCTGATTCACCAGCTGAGCCGTCGCCGCAGCCAGAGTCCGTTCCGCCGCAGCCACGGACAGGTGCAGC
  GAGTGGCCTTCCACCCTGCCCGGCCCTTCCTGTTGGTGGCGTCCCAGCGCAGCGTCCGCCTCTACCACCT
  GCTGCGCCAGGAGCTCACCAAGAAGCTGATGCCCAACTGCAAGTGGGTGTCCAGCCTGGCGGTGCACCCT
  GCAGGTGACAACGTCATCTGTGGGAGCTACGATAGCAAGCTGGTGTGGTTTGACCTGGATCTTTCCACCA
  AGCCATACAGGATGCTGAGACACCACAAGAAGGCTCTGCGGGCTGTGGCCTTCCACCCGCGGTACCCACT
  CTTTGCGTCAGGCTCGGACGACGGCAGTGTCATCGTCTGCCATGGCATGGTGTACAATGACCTTCTGCAG
  AACCCCTTGCTGGTGCCCGTCAAGGTGCTGAAGGGACACGTGCTGACCCGAGATCTGGGAGTGCTGGACG
  TCATCTTCCACCCCACCCAGCCGTGGGTCTTCTCCTCGGGGGCAGACGGGACTGTCCGCCTCTTCACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204016 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGSRGAGRTAAPSVRPEKRRSEPELEPEPEPEPPLLCTSPLSHSTGSDSGVSDSEESVFSGLEDSGSDS
  SEDDDEGDEEGEDGALDDEGHSGIKKTTEEQVQASTPCPRTEMASARIGDEYAEDSSDEEDIRNTVGNVP
  LEWYDDFPHVGYDLDGRRIYKPLRTRDELDQFLDKMDDPDYWRTVQDPMTGRDLRLTDEQVALVRRLQSG
  QFGDVGFNPYEPAVDFFSGDVMIHPVTNRPADKRSFIPSLVEKEKVSRMVHAIKMGWIQPRRPRDPTPSF
  YDLWAQEDPNAVLGRHKMHVPAPKLALPGHAESYNPPPEYLLSEEERLAWEQQEPGERKLSFLPRKFPSL
  RAVPAYGRFIQERFERCLDLYLCPRQRKMRVNVDPEDLIPKLPRPRDLQPFPTCQALVYRGHSDLVRCLS
  VSPGGQWLVSGSDDGSLRLWEVATARCVRTVPVGGVVKSVAWNPSPAVCLVAAAVEDSVLLLNPALGDRL
  VAGSTDQLLSAFVPPEEPPLQPARWLEASEEERQVGLRLRICHGKPVTQVTWHGRGDYLAVVLATQGHTQ
  VLIHQLSRRRSQSPFRRSHGQVQRVAFHPARPFLLVASQRSVRLYHLLRQELTKKLMPNCKWVSSLAVHP
  AGDNVICGSYDSKLVWFDLDLSTKPYRMLRHHKKALRAVAFHPRYPLFASGSDDGSVIVCHGMVYNDLLQ
  NPLLVPVKVLKGHVLTRDLGVLDVIFHPTQPWVFSSGADGTVRLFT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015201
• ORF Size: 2238 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015201.3, NP_056016.1
• RefSeq Size: 2422 bp
• RefSeq ORF: 2241 bp
• Locus ID: 23246
• UniProt ID: Q14137
• Cytogenetics: 8q24.3
• Domains: WD40
• MW: 83.6 kDa
• Gene Summary: Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.[UniProtKB/Swiss-Prot Function]