BOP1 (NM_015201) Human Tagged ORF Clone

CAT#: RC204016

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BOP1 (Myc-DDK-tagged)-Human block of proliferation 1 (BOP1)



Reconstitution Protocol

USD 683.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol BOP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC204016 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGTTCGCGGGGTGCGGGGCGCACGGCGGCGCCGAGCGTGCGGCCGGAGAAGCGGCGGTCTGAGC
CCGAACTGGAGCCTGAGCCCGAGCCGGAGCCCCCCCTCCTCTGCACCTCTCCTCTCAGCCACAGCACCGG
CAGCGATTCTGGCGTCTCCGACAGCGAGGAGAGTGTGTTCTCAGGCCTGGAAGATTCCGGCAGTGACAGC
AGTGAGGATGATGACGAAGGCGACGAGGAGGGAGAGGACGGAGCCCTTGATGACGAGGGCCACAGTGGGA
TTAAAAAGACCACTGAGGAGCAGGTGCAGGCCAGCACTCCTTGCCCGAGGACAGAGATGGCGAGCGCCCG
GATTGGGGATGAGTATGCGGAGGACAGCTCTGATGAGGAGGACATCCGGAACACGGTGGGCAACGTGCCC
TTGGAGTGGTACGATGACTTCCCCCACGTGGGCTACGACCTGGATGGCAGGCGCATCTACAAGCCCCTGC
GGACCCGGGATGAGCTGGACCAGTTCCTGGACAAGATGGACGATCCTGACTACTGGCGCACCGTGCAGGA
CCCGATGACAGGGCGGGACCTGAGACTGACGGATGAGCAGGTGGCCCTGGTGCGGCGGCTGCAGAGTGGC
CAGTTTGGGGATGTGGGCTTCAACCCCTATGAGCCGGCTGTCGACTTCTTCAGCGGGGACGTCATGATCC
ACCCGGTGACCAACCGCCCGGCCGACAAGCGCAGCTTCATCCCCTCCCTGGTGGAGAAGGAGAAGGTCTC
TCGCATGGTGCACGCCATCAAGATGGGCTGGATCCAGCCTCGCCGGCCCCGAGACCCCACCCCCAGCTTC
TATGACCTGTGGGCCCAGGAGGACCCCAACGCCGTGCTCGGGCGCCACAAGATGCACGTACCTGCTCCCA
AGCTGGCCCTGCCAGGCCACGCCGAGTCGTACAACCCACCCCCTGAATACCTGCTCAGCGAGGAGGAGCG
CTTGGCGTGGGAACAGCAGGAGCCAGGCGAGAGGAAGCTGAGCTTTTTGCCACGCAAGTTCCCGAGCCTG
CGGGCCGTGCCTGCCTACGGACGCTTCATCCAGGAACGCTTCGAGCGCTGCCTTGACCTGTACCTGTGCC
CACGGCAGCGCAAGATGAGGGTGAATGTAGACCCTGAGGACCTCATCCCCAAGCTGCCTCGGCCGAGGGA
CCTGCAGCCCTTCCCCACGTGCCAGGCCCTGGTCTACAGGGGCCACAGTGACCTTGTCCGGTGCCTCAGT
GTCTCTCCTGGGGGCCAGTGGCTGGTTTCAGGCTCTGACGACGGCTCCCTGCGGCTCTGGGAGGTGGCCA
CTGCCCGCTGTGTGAGGACTGTTCCCGTGGGGGGCGTGGTGAAGAGTGTGGCCTGGAACCCCAGCCCCGC
TGTCTGCCTGGTGGCTGCAGCCGTGGAGGACTCGGTGCTGCTGCTGAACCCAGCTCTGGGGGACCGGCTG
GTGGCGGGCAGCACAGATCAGCTGTTGAGCGCCTTCGTCCCGCCTGAGGAGCCCCCCTTGCAGCCGGCCC
GCTGGCTGGAGGCCTCAGAGGAGGAGCGCCAAGTGGGCCTGCGGCTGCGCATCTGCCACGGGAAGCCAGT
GACGCAGGTGACCTGGCACGGGCGTGGGGACTACCTGGCCGTGGTGCTGGCCACCCAAGGCCACACCCAG
GTGCTGATTCACCAGCTGAGCCGTCGCCGCAGCCAGAGTCCGTTCCGCCGCAGCCACGGACAGGTGCAGC
GAGTGGCCTTCCACCCTGCCCGGCCCTTCCTGTTGGTGGCGTCCCAGCGCAGCGTCCGCCTCTACCACCT
GCTGCGCCAGGAGCTCACCAAGAAGCTGATGCCCAACTGCAAGTGGGTGTCCAGCCTGGCGGTGCACCCT
GCAGGTGACAACGTCATCTGTGGGAGCTACGATAGCAAGCTGGTGTGGTTTGACCTGGATCTTTCCACCA
AGCCATACAGGATGCTGAGACACCACAAGAAGGCTCTGCGGGCTGTGGCCTTCCACCCGCGGTACCCACT
CTTTGCGTCAGGCTCGGACGACGGCAGTGTCATCGTCTGCCATGGCATGGTGTACAATGACCTTCTGCAG
AACCCCTTGCTGGTGCCCGTCAAGGTGCTGAAGGGACACGTGCTGACCCGAGATCTGGGAGTGCTGGACG
TCATCTTCCACCCCACCCAGCCGTGGGTCTTCTCCTCGGGGGCAGACGGGACTGTCCGCCTCTTCACC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC204016 protein sequence
Red=Cloning site Green=Tags(s)

MAGSRGAGRTAAPSVRPEKRRSEPELEPEPEPEPPLLCTSPLSHSTGSDSGVSDSEESVFSGLEDSGSDS
SEDDDEGDEEGEDGALDDEGHSGIKKTTEEQVQASTPCPRTEMASARIGDEYAEDSSDEEDIRNTVGNVP
LEWYDDFPHVGYDLDGRRIYKPLRTRDELDQFLDKMDDPDYWRTVQDPMTGRDLRLTDEQVALVRRLQSG
QFGDVGFNPYEPAVDFFSGDVMIHPVTNRPADKRSFIPSLVEKEKVSRMVHAIKMGWIQPRRPRDPTPSF
YDLWAQEDPNAVLGRHKMHVPAPKLALPGHAESYNPPPEYLLSEEERLAWEQQEPGERKLSFLPRKFPSL
RAVPAYGRFIQERFERCLDLYLCPRQRKMRVNVDPEDLIPKLPRPRDLQPFPTCQALVYRGHSDLVRCLS
VSPGGQWLVSGSDDGSLRLWEVATARCVRTVPVGGVVKSVAWNPSPAVCLVAAAVEDSVLLLNPALGDRL
VAGSTDQLLSAFVPPEEPPLQPARWLEASEEERQVGLRLRICHGKPVTQVTWHGRGDYLAVVLATQGHTQ
VLIHQLSRRRSQSPFRRSHGQVQRVAFHPARPFLLVASQRSVRLYHLLRQELTKKLMPNCKWVSSLAVHP
AGDNVICGSYDSKLVWFDLDLSTKPYRMLRHHKKALRAVAFHPRYPLFASGSDDGSVIVCHGMVYNDLLQ
NPLLVPVKVLKGHVLTRDLGVLDVIFHPTQPWVFSSGADGTVRLFT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_015201
ORF Size 2238 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_015201.3, NP_056016.1
RefSeq Size 2422 bp
RefSeq ORF 2241 bp
Locus ID 23246
UniProt ID Q14137
Cytogenetics 8q24.3
Domains WD40
MW 83.6 kDa
Gene Summary Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.[UniProtKB/Swiss-Prot Function]
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