IMP4 (NM_033416) Human Tagged ORF Clone
CAT#: RC204015
IMP4 (Myc-DDK-tagged)-Human IMP4, U3 small nucleolar ribonucleoprotein, homolog (yeast) (IMP4)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_033416" in other vectors (4)
USD 198.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | IMP4 |
Synonyms | BXDC4 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC204015 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCGCCGCGAGGCCCGCCTGCGCCGCGAGTACCTGTACCGCAAGGCCCGGGAGGAGGCGCAGCGCT CAGCCCAGGAGAGGAAGGAGCGGCTGCGGCGCGCGCTGGAAGAAAACCGCCTGATTCCCACTGAGTTACG CCGAGAGGCTCTGGCCTTACAGGGGTCCCTGGAGTTTGATGATGCTGGAGGTGAAGGTGTGACCAGCCAC GTGGATGATGAATACCGATGGGCAGGAGTCGAGGATCCCAAGGTTATGATCACTACCTCCCGAGACCCCA GTTCCCGCCTCAAGATGTTTGCAAAGGAGCTGAAGCTGGTGTTCCCGGGCGCCCAGCGAATGAACCGAGG TCGACATGAAGTGGGGGCACTGGTGCGAGCCTGCAAAGCCAACGGCGTCACCGATCTGCTGGTCGTTCAC GAGCATCGGGGCACACCTGTGGGGCTCATCGTCAGCCACCTGCCCTTTGGTCCTACTGCCTACTTCACGC TGTGCAATGTGGTCATGCGGCATGACATCCCAGACCTGGGCACCATGTCGGAGGCCAAGCCCCACCTCAT CACACACGGCTTCTCCTCCCGCCTGGGCAAGCGGGTCTCTGACATCCTCCGATACCTATTTCCCGTGCCC AAAGATGACAGCCACCGGGTCATCACCTTCGCAAACCAGGACGACTACATATCATTCCGGCACCATGTGT ATAAGAAGACAGACCACCGCAACGTGGAGCTCACTGAGGTCGGGCCCCGCTTTGAGCTGAAGCTGTACAT GATCCGTCTGGGCACGCTGGAGCAGGAGGCCACAGCAGACGTGGAGTGGCGCTGGCACCCTTACACCAAT ACCGCACGCAAGAGAGTCTTCCTGAGCACCGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204015 protein sequence
Red=Cloning site Green=Tags(s) MLRREARLRREYLYRKAREEAQRSAQERKERLRRALEENRLIPTELRREALALQGSLEFDDAGGEGVTSH VDDEYRWAGVEDPKVMITTSRDPSSRLKMFAKELKLVFPGAQRMNRGRHEVGALVRACKANGVTDLLVVH EHRGTPVGLIVSHLPFGPTAYFTLCNVVMRHDIPDLGTMSEAKPHLITHGFSSRLGKRVSDILRYLFPVP KDDSHRVITFANQDDYISFRHHVYKKTDHRNVELTEVGPRFELKLYMIRLGTLEQEATADVEWRWHPYTN TARKRVFLSTE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_033416 |
ORF Size | 873 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_033416.3 |
RefSeq Size | 1074 bp |
RefSeq ORF | 876 bp |
Locus ID | 92856 |
UniProt ID | Q96G21 |
Cytogenetics | 2q21.1 |
Domains | Brix |
MW | 33.8 kDa |
Gene Summary | The protein encoded by this gene, along with IMP3 and MPP10, is part of the 60-80S U3 small nucleolar ribonucleoprotein (U3 snoRNP) complex. This complex is necessary for the early cleavage steps of pre-18S ribosomal RNA processing. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204015L3 | Lenti ORF clone of Human IMP4, U3 small nucleolar ribonucleoprotein, homolog (yeast) (IMP4), Myc-DDK-tagged |
USD 600.00 |
|
RC204015L4 | Lenti ORF clone of Human IMP4, U3 small nucleolar ribonucleoprotein, homolog (yeast) (IMP4), mGFP tagged |
USD 600.00 |
|
RG204015 | IMP4 (tGFP-tagged) - Human IMP4, U3 small nucleolar ribonucleoprotein, homolog (yeast) (IMP4) |
USD 500.00 |
|
SC127736 | IMP4 (untagged)-Human IMP4, U3 small nucleolar ribonucleoprotein, homolog (yeast) (IMP4) |
USD 300.00 |
{0} Product Review(s)
Be the first one to submit a review