CORO2A (NM_052820) Human Tagged ORF Clone

CAT#: RC203993

CORO2A (Myc-DDK-tagged)-Human coronin, actin binding protein, 2A (CORO2A), transcript variant 2

Product Images

Circular map for RC203993

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  Western blot validation of overexpression lysate (Cat# LY403258) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203993 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CORO2A protein (Cat# TP303993). The protein was produced from HEK293T cells transfected with CORO2A cDNA clone (Cat# RC203993) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CORO2A
• Synonyms: CLIPINB; IR10; WDR2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203993 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCATGGCACCCCCAGTACCGGAGCTCCAAGTTCCGTCATGTCTTTGGCAAACCAGCCAGCAAGGAGA
  ACTGCTACGACTCCGTGCCTATCACCCGCAGCGTTCACGACAACCACTTCTGTGCCGTGAACCCCCACTT
  CATTGCAGTTGTGACTGAGTGTGCTGGTGGAGGGGCCTTCCTCGTCATCCCCCTGCACCAGACAGGGAAG
  TTGGACCCCCACTACCCAAAAGTCTGCGGGCACAGAGGCAACGTTTTGGATGTCAAGTGGAACCCTTTTG
  ATGATTTTGAGATCGCCTCCTGTTCTGAAGATGCCACAATTAAGATCTGGAGCATCCCCAAGCAGCTGCT
  GACCAGGAACCTCACGGCCTACAGGAAGGAACTCGTGGGCCACGCGCGCAGAGTAGGCCTGGTGGAGTGG
  CACCCCACGGCCGCCAACATCCTCTTCAGTGCTGGCTATGACTACAAGGTGATGATCTGGAACCTGGATA
  CAAAGGAGTCTGTCATCACAAGCCCCATGAGTACAATTAGCTGTCACCAAGATGTGATCCTCTCCATGTC
  CTTCAACACCAACGGCAGCCTGTTGGCCACCACCTGCAAAGACCGCAAGATTCGGGTTATTGACCCCCGA
  GCAGGGACCGTCCTCCAGGAGGCCAGCTACAAAGGGCACCGGGCCAGCAAAGTGCTGTTTCTGGGGAACC
  TGAAGAAGCTGATGTCCACAGGCACATCCCGATGGAACAACCGGCAGGTGGCCTTGTGGGACCAGGATAA
  CCTCTCTGTGCCTCTGATGGAGGAGGACCTGGACGGCTCCTCGGGCGTGCTGTTTCCCTTCTATGACGCG
  GACACCAGCATGCTCTACGTGGTGGGGAAGGGAGATGGCAACATCCGCTACTACGAGGTGAGCGCCGACA
  AGCCTCACCTGAGCTACCTGACTGAGTACCGCTCCTATAACCCACAGAAGGGGATCGGTGTCATGCCAAA
  GAGAGGACTCGACGTGTCCTCCTGCGAGATCTTCCGCTTCTACAAGCTGATCACAACCAAAAGCCTCATC
  GAGCCCATCTCCATGATTGTGCCCCGGCGGTCAGAATCCTACCAAGAGGACATATACCCTCCAACAGCAG
  GGGCCCAGCCCTCCCTGACGGCCCAGGAGTGGCTCAGCGGGATGAATCGAGACCCAATCCTGGTGTCCCT
  TAGGCCTGGCTCTGAGCTGCTGAGACCCCACCCACTGCCTGCAGAGAGACCTATCTTCAATTCCATGGCC
  CCAGCCTCACCCCGGCTCTTGAATCAGACAGAAAAGCTGGCTGCAGAAGATGGCTGGAGGTCTTCCTCCC
  TGTTGGAGGAGAAGATGCCAAGGTGGGCAGCAGAACACAGGCTGGAGGAGAAGAAAACCTGGCTGACAAA
  TGGCTTTGACGTTTTCGAATGCCCCCCACCAAAGACAGAGAATGAGTTGCTGCAGATGTTCTACCGGCAA
  CAGGAGGAGATCCGAAGGCTCCGGGAGCTGTTGACCCAGCGAGAGGTCCAGGCCAAACAGTTGGAACTGG
  AGATCAAAAACTTGCGGATGGGCTCAGAGCAGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203993 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSWHPQYRSSKFRHVFGKPASKENCYDSVPITRSVHDNHFCAVNPHFIAVVTECAGGGAFLVIPLHQTGK
  LDPHYPKVCGHRGNVLDVKWNPFDDFEIASCSEDATIKIWSIPKQLLTRNLTAYRKELVGHARRVGLVEW
  HPTAANILFSAGYDYKVMIWNLDTKESVITSPMSTISCHQDVILSMSFNTNGSLLATTCKDRKIRVIDPR
  AGTVLQEASYKGHRASKVLFLGNLKKLMSTGTSRWNNRQVALWDQDNLSVPLMEEDLDGSSGVLFPFYDA
  DTSMLYVVGKGDGNIRYYEVSADKPHLSYLTEYRSYNPQKGIGVMPKRGLDVSSCEIFRFYKLITTKSLI
  EPISMIVPRRSESYQEDIYPPTAGAQPSLTAQEWLSGMNRDPILVSLRPGSELLRPHPLPAERPIFNSMA
  PASPRLLNQTEKLAAEDGWRSSSLLEEKMPRWAAEHRLEEKKTWLTNGFDVFECPPPKTENELLQMFYRQ
  QEEIRRLRELLTQREVQAKQLELEIKNLRMGSEQL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_052820
• ORF Size: 1575 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_052820.4
• RefSeq Size: 5507 bp
• RefSeq ORF: 1578 bp
• Locus ID: 7464
• UniProt ID: Q92828
• Cytogenetics: 9q22.33
• Domains: WD40
• MW: 59.8 kDa
• Gene Summary: This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains 5 WD repeats, and has a structural similarity with actin-binding proteins: the D. discoideum coronin and the human p57 protein, suggesting that this protein may also be an actin-binding protein that regulates cell motility. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008]