GIMAP5 (NM_018384) Human Tagged ORF Clone

CAT#: RC203978

GIMAP5 (Myc-DDK-tagged)-Human GTPase, IMAP family member 5 (GIMAP5)

Product Images

Circular map for RC203978

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  Western blot validation of overexpression lysate (Cat# LY413083) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203978 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GIMAP5 protein (Cat# TP303978). The protein was produced from HEK293T cells transfected with GIMAP5 cDNA clone (Cat# RC203978) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GIMAP5
• Synonyms: HIMAP3; IAN-5; IAN4; IAN4L1; IAN5; IMAP3; IROD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203978 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGGATTCCAGAGGGGCAAATATGGAACTATGGCTGAAGGTAGATCAGAAGATAACTTGTCTGCAA
  CACCACCGGCATTGAGGATTATCCTAGTGGGCAAAACAGGCTGCGGGAAAAGTGCCACAGGGAACAGCAT
  CCTTGGCCAGCCCGTGTTTGAGTCCAAGCTGAGGGCCCAGTCAGTGACCAGGACGTGCCAGGTGAAAACA
  GGAACATGGAACGGGAGGAAAGTCCTGGTGGTTGACACGCCCTCCATCTTTGAGTCACAGGCCGATACCC
  AAGAGCTGTACAAGAACATCGGGGACTGCTACCTGCTCTCTGCCCCGGGGCCCCACGTCCTGCTTCTGGT
  GATCCAGCTGGGGCGTTTCACTGCTCAGGACACAGTGGCCATCAGGAAGGTGAAAGAGGTCTTTGGGACA
  GGGGCCATGAGACATGTGGTCATCCTCTTCACCCACAAAGAGGACTTAGGGGGCCAGGCCCTGGATGACT
  ATGTAGCAAACACGGACAACTGCAGCCTGAAAGACCTGGTGCGGGAGTGTGAGAGAAGGTACTGTGCCTT
  CAACAACTGGGGCTCTGTGGAGGAGCAGAGGCAGCAGCAGGCAGAGCTCCTGGCTGTGATTGAGAGGCTG
  GGGAGGGAGCGAGAGGGCTCCTTCCACAGCAATGACCTCTTCTTGGATGCCCAGCTGCTCCAAAGAACTG
  GAGCTGGGGCCTGCCAGGAAGACTACAGGCAGTACCAGGCCAAAGTGGAATGGCAGGTGGAGAAGCACAA
  GCAAGAGCTGAGGGAGAACGAGAGTAACTGGGCATACAAGGCGCTCCTCAGAGTCAAACACTTGATGCTT
  TTGCATTATGAGATTTTTGTTTTTCTATTGTTGTGCAGCATACTTTTTTTCATTATTTTTCTGTTCATCT
  TTCATTACATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203978 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGGFQRGKYGTMAEGRSEDNLSATPPALRIILVGKTGCGKSATGNSILGQPVFESKLRAQSVTRTCQVKT
  GTWNGRKVLVVDTPSIFESQADTQELYKNIGDCYLLSAPGPHVLLLVIQLGRFTAQDTVAIRKVKEVFGT
  GAMRHVVILFTHKEDLGGQALDDYVANTDNCSLKDLVRECERRYCAFNNWGSVEEQRQQQAELLAVIERL
  GREREGSFHSNDLFLDAQLLQRTGAGACQEDYRQYQAKVEWQVEKHKQELRENESNWAYKALLRVKHLML
  LHYEIFVFLLLCSILFFIIFLFIFHYI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018384
• ORF Size: 921 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018384.4
• RefSeq Size: 1895 bp
• RefSeq ORF: 924 bp
• Locus ID: 55340
• UniProt ID: Q96F15
• Cytogenetics: 7q36.1
• Protein Families: Transmembrane
• MW: 34.8 kDa
• Gene Summary: This gene encodes a protein belonging to the GTP-binding superfamily and to the immuno-associated nucleotide (IAN) subfamily of nucleotide-binding proteins. In humans, the IAN subfamily genes are located in a cluster at 7q36.1. This gene encodes an antiapoptotic protein that functions in T-cell survival. Polymorphisms in this gene are associated with systemic lupus erythematosus. Read-through transcription exists between this gene and the neighboring upstream GIMAP1 (GTPase, IMAP family member 1) gene. [provided by RefSeq, Dec 2010]