RNF10 (NM_014868) Human Tagged ORF Clone

CAT#: RC203905

RNF10 (Myc-DDK-tagged)-Human ring finger protein 10 (RNF10)

Product Images

Circular map for RC203905

• 

  Western blot validation of overexpression lysate (Cat# LY414976) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203905 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RNF10
• Synonyms: RIE2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203905 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCTGAGCTCCCCCAACGCCGCCGCCACCGCCTCCGACATGGACAAGAACAGCGGCTCCAACAGCT
  CCTCCGCCTCTTCGGGCAGCAGCAAAGGGCAACAGCCGCCCCGCTCCGCCTCGGCGGGGCCAGCCGGCGA
  GTCTAAACCCAAGAGCGATGGAAAGAACTCCAGTGGATCCAAGCGTTATAATCGCAAACGTGAACTTTCC
  TACCCCAAAAATGAAAGTTTTAACAACCAGTCCCGTCGCTCCAGTTCACAGAAAAGCAAGACTTTTAACA
  AGATGCCTCCTCAAAGGGGCGGCGGCAGCAGCAAACTCTTTAGCTCTTCTTTTAATGGTGGAAGACGAGA
  TGAGGTAGCAGAGGCTCAACGGGCAGAGTTTAGCCCTGCCCAGTTCTCTGGTCCTAAGAAGATCAACCTG
  AACCACTTGTTGAATTTCACTTTTGAACCCCGTGGCCAGACGGGTCACTTTGAAGGCAGTGGACATGGTA
  GCTGGGGAAAGAGGAACAAGTGGGGACATAAGCCTTTTAACAAGGAACTCTTTTTACAGGCCAACTGCCA
  ATTTGTGGTGTCTGAAGACCAAGACTACACAGCTCATTTTGCTGATCCTGATACATTAGTTAACTGGGAC
  TTTGTGGAACAAGTGCGCATTTGTAGCCATGAAGTGCCATCTTGCCCAATATGCCTCTATCCACCTACTG
  CAGCCAAGATAACCCGTTGTGGACACATCTTCTGCTGGGCATGCATCCTGCACTATCTTTCACTGAGTGA
  GAAGACGTGGAGTAAATGTCCCATCTGTTACAGTTCTGTGCATAAGAAGGATCTCAAGAGTGTTGTTGCC
  ACAGAGTCACATCAGTATGTTGTTGGTGATACCATTACGATGCAGCTGATGAAGAGGGAGAAAGGGGTGT
  TGGTGGCTTTGCCCAAATCCAAATGGATGAATGTAGACCATCCCATTCATCTAGGAGATGAACAGCACAG
  CCAGTACTCCAAGTTGCTGCTGGCCTCTAAGGAGCAGGTGCTGCACCGGGTAGTTCTGGAGGAGAAAGTA
  GCACTAGAGCAGCAGCTGGCAGAGGAGAAGCACACTCCCGAGTCCTGCTTTATTGAGGCAGCTATCCAGG
  AGCTCAAGACTCGGGAAGAGGCTCTGTCGGGATTGGCCGGAAGCAGAAGGGAGGTCACTGGTGTTGTGGC
  TGCTCTGGAACAACTGGTGCTGATGGCTCCCTTGGCGAAGGAGTCTGTTTTTCAACCCAGGAAGGGTGTG
  CTGGAGTATCTGTCTGCCTTCGATGAAGAAACCACGGAAGTTTGTTCTCTGGACACTCCTTCTAGACCTC
  TTGCTCTCCCTCTGGTAGAAGAGGAGGAAGCAGTGTCTGAACCAGAGCCTGAGGGGTTGCCAGAGGCCTG
  TGATGACTTGGAGTTAGCAGATGACAATCTTAAAGAGGGGACCATTTGCACTGAGTCCAGCCAGCAGGAA
  CCCATCACCAAGTCAGGCTTCACACGCCTCAGCAGCTCTCCTTGTTACTACTTTTACCAAGCGGAAGATG
  GACAGCATATGTTCCTGCACCCTGTGAATGTGCGCTGCCTCGTGCGGGAGTACGGCAGCCTGGAGAGGAG
  CCCCGAGAAGATCTCAGCAACTGTGGTGGAGATTGCTGGCTACTCCATGTCTGAGGATGTTCGACAGCGT
  CACAGATATCTCTCTCACTTGCCACTCACCTGTGAGTTCAGCATCTGTGAACTGGCTTTGCAACCTCCTG
  TGGTCTCTAAGGAAACCCTAGAGATGTTCTCAGATGACATTGAGAAGAGGAAACGTCAGCGCCAAAAGAA
  GGCTCGGGAGGAACGCCGCCGAGAGCGCAGGATTGAGATAGAGGAGAACAAGAAACAGGGCAAGTACCCA
  GAAGTCCACATTCCCCTCGAGAATCTACAGCAGTTTCCTGCCTTCAATTCTTATACCTGCTCCTCTGATT
  CTGCTTTGGGTCCCACCAGCACCGAGGGCCATGGGGCCCTCTCCATTTCTCCTCTCAGCAGAAGTCCAGG
  TTCCCATGCAGACTTTCTGCTGACCCCTCTGTCACCCACTGCCAGTCAGGGCAGTCCCTCATTCTGCGTT
  GGGAGTCTGGAAGAAGACTCTCCCTTCCCTTCCTTTGCCCAGATGCTGAGGGTTGGAAAAGCAAAAGCAG
  ATGTGTGGCCCAAAACTGCTCCAAAGAAAGATGAGAACAGCTTAGTTCCTCCTGCCCCTGTGGACAGCGA
  CGGGGAGAGTGATAATTCAGACCGTGTTCCTGTGCCCAGTTTTCAAAATTCCTTCAGCCAAGCTATTGAA
  GCAGCCTTCATGAAACTGGACACACCAGCTACTTCAGATCCCCTCTCTGAAGAGAAAGGAGGAAAGAAAA
  GAAAAAAACAGAAACAGAAGCTCCTGTTCAGCACCTCAGTCGTCCACACCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203905 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPLSSPNAAATASDMDKNSGSNSSSASSGSSKGQQPPRSASAGPAGESKPKSDGKNSSGSKRYNRKRELS
  YPKNESFNNQSRRSSSQKSKTFNKMPPQRGGGSSKLFSSSFNGGRRDEVAEAQRAEFSPAQFSGPKKINL
  NHLLNFTFEPRGQTGHFEGSGHGSWGKRNKWGHKPFNKELFLQANCQFVVSEDQDYTAHFADPDTLVNWD
  FVEQVRICSHEVPSCPICLYPPTAAKITRCGHIFCWACILHYLSLSEKTWSKCPICYSSVHKKDLKSVVA
  TESHQYVVGDTITMQLMKREKGVLVALPKSKWMNVDHPIHLGDEQHSQYSKLLLASKEQVLHRVVLEEKV
  ALEQQLAEEKHTPESCFIEAAIQELKTREEALSGLAGSRREVTGVVAALEQLVLMAPLAKESVFQPRKGV
  LEYLSAFDEETTEVCSLDTPSRPLALPLVEEEEAVSEPEPEGLPEACDDLELADDNLKEGTICTESSQQE
  PITKSGFTRLSSSPCYYFYQAEDGQHMFLHPVNVRCLVREYGSLERSPEKISATVVEIAGYSMSEDVRQR
  HRYLSHLPLTCEFSICELALQPPVVSKETLEMFSDDIEKRKRQRQKKAREERRRERRIEIEENKKQGKYP
  EVHIPLENLQQFPAFNSYTCSSDSALGPTSTEGHGALSISPLSRSPGSHADFLLTPLSPTASQGSPSFCV
  GSLEEDSPFPSFAQMLRVGKAKADVWPKTAPKKDENSLVPPAPVDSDGESDNSDRVPVPSFQNSFSQAIE
  AAFMKLDTPATSDPLSEEKGGKKRKKQKQKLLFSTSVVHTK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014868
• ORF Size: 2433 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014868.5
• RefSeq Size: 3864 bp
• RefSeq ORF: 2436 bp
• Locus ID: 9921
• UniProt ID: Q8N5U6
• Cytogenetics: 12q24.31
• Domains: RING
• Protein Families: Druggable Genome
• MW: 89.9 kDa
• Gene Summary: The protein encoded by this gene contains a ring finger motif, which is known to be involved in protein-protein interactions. The specific function of this protein has not yet been determined. EST data suggests the existence of multiple alternatively spliced transcript variants, however, their full length nature is not known. [provided by RefSeq, Jul 2008]