FAM108A1 (ABHD17A) (NM_031213) Human Tagged ORF Clone

CAT#: RC203884

FAM108A1 (Myc-DDK-tagged)-Human family with sequence similarity 108, member A1 (FAM108A1), transcript variant 1

Product Images

Circular map for RC203884

• 

  Western blot validation of overexpression lysate (Cat# LY403109) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203884 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FAM108A1
• Synonyms: C19orf27; FAM108A1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203884 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGGGCTGTCGCTGAGTGAGCTCTGCTGCCTCTTCTGCTGCCCGCCCTGCCCCGGCCGCATCGCTG
  CCAAGCTCGCCTTCCTGCCGCCGGAGGCCACCTACTCCCTGGTGCCTGAGCCCGAGCCGGGGCCTGGTGG
  GGCCGGGGCCGCCCCCTTGGGGACCCTGAGAGCCTCCTCGGGCGCACCCGGGCGCTGGAAGCTGCACCTG
  ACGGAGCGTGCCGACTTCCAGTACAGCCAGCGCGAGCTGGACACCATCGAGGTCTTCCCCACCAAGAGCG
  CCCGCGGCAACCGCGTCTCCTGCATGTATGTTCGCTGCGTGCCTGGTGCCAGACAAGGACACCAGGCTCA
  GGGAGGCCATCCCCAGCTGGCATGGGTGGGCAGGCTGGGCGACTCCAACAACCCAGCGCCTGGTGGTTGC
  CTGCTGGGCGAGAGCTGGGGCACAGGGGCTGCCCTGGCCTGCGGGTACATCCACCTTCTCGCCAGGTACA
  CGGTCCTCTTCTCGCACGGCAATGCCGTGGACCTGGGCCAGATGAGCAGCTTCTACATTGGCCTGGGCTC
  CCGCCTCCACTGCAACATCTTCTCCTACGACTACTCCGGCTACGGTGCCAGCTCGGGCAGGCCTTCCGAG
  AGGAACCTCTATGCCGACATCGACGCCGCCTGGCAGGCCCTGCGCACCAGGTACGGCATCAGCCCGGACA
  GCATCATCCTGTACGGGCAGAGCATCGGCACGGTGCCCACCGTGGACCTGGCCTCGCGCTACGAGTGTGC
  CGCGGTGGTGCTGCACTCGCCGCTCACCTCGGGCATGCGCGTCGCCTTCCCCGACACCAAGAAGACCTAC
  TGCTTCGACGCCTTCCCTAACATCGAGAAGGTGTCCAAGATCACGTCTCCCGTGCTCATCATCCACGGCA
  CGGAGGACGAGGTGATCGACTTCTCGCACGGGCTGGCGCTCTACGAGCGCTGCCCCAAGGCGGTGGAGCC
  GCTGTGGGTGGAGGGCGCCGGGCACAACGACATCGAGCTCTACAGCCAGTACCTGGAGCGCCTGCGTCGC
  TTCATCTCCCAGGAGCTGCCCAGCCAGCGCGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203884 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNGLSLSELCCLFCCPPCPGRIAAKLAFLPPEATYSLVPEPEPGPGGAGAAPLGTLRASSGAPGRWKLHL
  TERADFQYSQRELDTIEVFPTKSARGNRVSCMYVRCVPGARQGHQAQGGHPQLAWVGRLGDSNNPAPGGC
  LLGESWGTGAALACGYIHLLARYTVLFSHGNAVDLGQMSSFYIGLGSRLHCNIFSYDYSGYGASSGRPSE
  RNLYADIDAAWQALRTRYGISPDSIILYGQSIGTVPTVDLASRYECAAVVLHSPLTSGMRVAFPDTKKTY
  CFDAFPNIEKVSKITSPVLIIHGTEDEVIDFSHGLALYERCPKAVEPLWVEGAGHNDIELYSQYLERLRR
  FISQELPSQRA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031213
• ORF Size: 1083 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031213.2, NP_112490.2
• RefSeq Size: 1731 bp
• RefSeq ORF: 1086 bp
• Locus ID: 81926
• UniProt ID: Q96GS6
• Cytogenetics: 19p13.3
• Protein Families: Protease
• MW: 39.1 kDa
• Gene Summary: Hydrolyzes fatty acids from S-acylated cysteine residues in proteins (PubMed:26701913). Has depalmitoylating activity towards NRAS (PubMed:26701913). Has depalmitoylating activity towards DLG4/PSD95 (PubMed:26701913). May have depalmitoylating activity towards MAP6 (By similarity).[UniProtKB/Swiss-Prot Function]