POP1 (NM_015029) Human Tagged ORF Clone

CAT#: RC203861

POP1 (Myc-DDK-tagged)-Human processing of precursor 1, ribonuclease P/MRP subunit (S. cerevisiae) (POP1), transcript variant 3

Product Images

Circular map for RC203861

• 

  Western blot validation of overexpression lysate (Cat# LY429038) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226611 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: POP1
• Synonyms: ANXD2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203861 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAAATGCAAAAGAAAGAAAACACGCCAAGAAAATGAGAAACCAGCCTACCAATGTGACTCTGTCCT
  CTGGCTTTGTGGCTGACAGAGGTGTAAAGCACCACAGTGGAGGTGAAAAACCTTTCCAAGCTCAAAAACA
  AGAGCCTCATCCTGGAACTTCACGACAGCGGCAAACCAGAGTCAACCCCCATTCTCTGCCTGACCCTGAA
  GTGAATGAGCAGTCTTCCTCCAAAGGGATGTTTAGAAAAAAGGGAGGATGGAAAGCAGGTCCCGAGGGCA
  CGTCTCAGGAGATCCCCAAGTATATAACTGCTTCTACTTTTGCTCAAGCACGAGCTGCTGAAATCAGTGC
  TATGTTAAAAGCTGTGACCCAGAAGTCTTCGAATTCACTGGTTTTTCAGACTCTGCCACGGCACATGCGA
  CGAAGAGCCATGAGCCACAACGTCAAACGCCTTCCCAGACGGTTACAGGAGATTGCCCAGAAAGAGGCGG
  AGAAAGCCGTACATCAGAAAAAAGAACATTCAAAAAATAAATGCCATAAAGCTCGAAGATGTCACATGAA
  CCGGACGCTAGAATTTAACCGTAGACAAAAGAAGAACATTTGGTTAGAAACTCACATCTGGCACGCCAAG
  CGGTTTCATATGGTCAAGAAGTGGGGCTACTGCCTTGGGGAGAGGCCAACAGTCAAGAGCCACAGAGCCT
  GCTATCGAGCCATGACGAACCGGTGCCTCCTGCAGGATTTATCCTATTACTGTTGTTTGGAGTTGAAAGG
  CAAAGAGGAAGAAATACTAAAGGCGCTTTCTGGAATGTGTAACATAGACACAGGGCTGACGTTTGCAGCA
  GTTCACTGCTTGTCTGGAAAGCGCCAAGGGAGCCTTGTGCTTTATCGGGTGAATAAATATCCCAGAGAAA
  TGCTTGGGCCTGTTACGTTTATCTGGAAGTCCCAGAGGACCCCGGGTGACCCTTCTGAGAGCAGGCAGCT
  GTGGATCTGGCTGCATCCAACCCTTAAACAGGATATCTTAGAGGAAATAAAAGCAGCGTGCCAGTGTGTG
  GAACCCATCAAATCAGCTGTCTGCATCGCTGACCCACTTCCAACACCATCCCAAGAAAAAAGCCAAACTG
  AATTGCCTGACGAGAAAATTGGCAAGAAAAGAAAAAGGAAAGATGATGGAGAAAATGCTAAACCAATTAA
  AAAAATTATCGGTGATGGAACTAGAGATCCATGTCTACCATACTCTTGGATCTCTCCAACCACAGGCATT
  ATAATCAGCGATTTGACGATGGAGATGAACAGATTCCGGCTGATTGGGCCACTTTCCCACTCCATCCTAA
  CTGAAGCAATAAAAGCTGCTTCTGTCCACACTGTGGGAGAGGACACAGAGGAGACACCTCACCGCTGGTG
  GATAGAAACCTGTAAGAAACCTGACAGCGTTTCCCTTCATTGCAGACAAGAAGCCATTTTCGAGTTGTTG
  GGAGGAATAACATCACCAGCAGAAATTCCGGCAGGTACTATTCTGGGACTGACAGTTGGGGATCCTCGAA
  TAAATTTGCCCCAAAAGAAGTCCAACGCTTTGCCCAATCCAGAAAAATGCCAAGATAATGAGAAAGTTAG
  ACAGCTGCTTCTGGAGGGTGTGCCTGTGGAATGTACGCATAGCTTTATCTGGAACCAAGATATCTGTAAG
  AGTGTCACAGAGAATAAAATCTCGGATCAGGATTTAAACCGGATGAGGAGTGAATTGCTGGTGCCTGGGT
  CACAGCTTATTTTAGGTCCCCATGAATCCAAGATACCTATACTTTTGATTCAGCAGCCAGGAAAAGTGAC
  TGGTGAAGATCGACTAGGCTGGGGAAGTGGCTGGGATGTCCTACTCCCAAAGGGCTGGGGCATGGCTTTC
  TGGATTCCATTTATTTATCGAGGTGTGAGAGTCGGAGGGTTGAAAGAGTCTGCAGTGCATTCTCAGTATA
  AGAGGTCGCCTAATGTCCCAGGCGATTTTCCAGACTGCCCTGCCGGGATGCTGTTTGCGGAAGAGCAAGC
  TAAGAATCTTCTTGAAAAGTACAAAAGACGCCCTCCTGCAAAACGGCCCAACTACGTTAAGCTTGGCACT
  CTGGCACCTTTCTGCTGTCCCTGGGAGCAGTTAACTCAAGACTGGGAGTCAAGAGTCCAGGCTTACGAAG
  AACCTTCTGTAGCTTCATCTCCAAATGGTAAGGAGAGTGACCTAAGAAGATCTGAGGTGCCTTGTGCTCC
  CATGCCTAAAAAAACTCATCAGCCATCTGATGAAGTGGGCACATCCATAGAGCACCCCAGGGAGGCAGAG
  GAGGTAATGGATGCAGGGTGTCAAGAATCGGCAGGGCCTGAGAGGATCACAGACCAGGAGGCCAGTGAAA
  ACCATGTTGCTGCCACAGGGAGTCACCTCTGCGTTCTCAGGAGTAGAAAATTACTGAAGCAACTGTCAGC
  CTGGTGTGGGCCCAGTTCTGAGGATAGTCGGGGAGGCCGGCGAGCTCCCGGCAGAGGCCAGCAAGGATTG
  ACCAGAGAGGCTTGCCTGTCCATCTTGGGCCACTTCCCCAGGGCCCTGGTTTGGGTCAGCCTGTCCCTGC
  TCAGCAAGGGCAGCCCCGAGCCTCACACCATGATCTGTGTCCCAGCCAAGGAGGACTTCCTCCAGCTCCA
  TGAGGACTGGCATTACTGTGGGCCCCAGGAATCCAAACACAGTGACCCATTCAGGAGCAAGATCCTGAAA
  CAGAAAGAGAAGAAGAAAAGGGAGAAGAGGCAGAAGCCAGGACGTGCCTCTTCTGATGGCCCGGCGGGGG
  AAGAGCCCGTGGCTGGGCAGGAAGCTCTGACTCTAGGGCTGTGGTCAGGCCCTCTGCCGCGTGTGACGTT
  GCACTGCTCCAGAACTCTCCTAGGCTTTGTGACTCAGGGAGATTTTTCCATGGCTGTTGGCTGTGGAGAA
  GCCCTGGGGTTTGTTAGCTTGACAGGCTTGCTGGATATGGTGTCCAGCCAGCCTGCAGCGCAGAGGGGCT
  TAGTGCTACTGAGGCCTCCCGCCTCTCTGCAGTATCGATTTGCGAGGATTGCTATTGAGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203861 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSNAKERKHAKKMRNQPTNVTLSSGFVADRGVKHHSGGEKPFQAQKQEPHPGTSRQRQTRVNPHSLPDPE
  VNEQSSSKGMFRKKGGWKAGPEGTSQEIPKYITASTFAQARAAEISAMLKAVTQKSSNSLVFQTLPRHMR
  RRAMSHNVKRLPRRLQEIAQKEAEKAVHQKKEHSKNKCHKARRCHMNRTLEFNRRQKKNIWLETHIWHAK
  RFHMVKKWGYCLGERPTVKSHRACYRAMTNRCLLQDLSYYCCLELKGKEEEILKALSGMCNIDTGLTFAA
  VHCLSGKRQGSLVLYRVNKYPREMLGPVTFIWKSQRTPGDPSESRQLWIWLHPTLKQDILEEIKAACQCV
  EPIKSAVCIADPLPTPSQEKSQTELPDEKIGKKRKRKDDGENAKPIKKIIGDGTRDPCLPYSWISPTTGI
  IISDLTMEMNRFRLIGPLSHSILTEAIKAASVHTVGEDTEETPHRWWIETCKKPDSVSLHCRQEAIFELL
  GGITSPAEIPAGTILGLTVGDPRINLPQKKSNALPNPEKCQDNEKVRQLLLEGVPVECTHSFIWNQDICK
  SVTENKISDQDLNRMRSELLVPGSQLILGPHESKIPILLIQQPGKVTGEDRLGWGSGWDVLLPKGWGMAF
  WIPFIYRGVRVGGLKESAVHSQYKRSPNVPGDFPDCPAGMLFAEEQAKNLLEKYKRRPPAKRPNYVKLGT
  LAPFCCPWEQLTQDWESRVQAYEEPSVASSPNGKESDLRRSEVPCAPMPKKTHQPSDEVGTSIEHPREAE
  EVMDAGCQESAGPERITDQEASENHVAATGSHLCVLRSRKLLKQLSAWCGPSSEDSRGGRRAPGRGQQGL
  TREACLSILGHFPRALVWVSLSLLSKGSPEPHTMICVPAKEDFLQLHEDWHYCGPQESKHSDPFRSKILK
  QKEKKKREKRQKPGRASSDGPAGEEPVAGQEALTLGLWSGPLPRVTLHCSRTLLGFVTQGDFSMAVGCGE
  ALGFVSLTGLLDMVSSQPAAQRGLVLLRPPASLQYRFARIAIEV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015029
• ORF Size: 3072 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015029.1, NP_055844.1
• RefSeq Size: 4689 bp
• RefSeq ORF: 3075 bp
• Locus ID: 10940
• UniProt ID: Q99575
• Cytogenetics: 8q22.2
• Protein Families: Stem cell - Pluripotency
• MW: 114.7 kDa
• Gene Summary: This gene encodes the protein subunit of two different small nucleolar ribonucleoprotein complexes: the endoribonuclease for mitochondrial RNA processing complex and the ribonuclease P complex. The encoded protein is a ribonuclease that localizes to the nucleus and functions in pre-RNA processing. This protein is also an autoantigen in patients suffering from connective tissue diseases. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2009]