MASTL (NM_032844) Human Tagged ORF Clone

CAT#: RC203858

MASTL (Myc-DDK-tagged)-Human microtubule associated serine/threonine kinase-like (MASTL), transcript variant 2

Product Images

Circular map for RC203858

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  Western blot validation of overexpression lysate (Cat# LY403204) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203858 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MASTL protein (Cat# TP303858). The protein was produced from HEK293T cells transfected with MASTL cDNA clone (Cat# RC203858) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MASTL
• Synonyms: GREATWALL; GW; GWL; MAST-L; THC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203858 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCCACCGCGGGAAGCAAGAAGGAGCCTGGAGGAGGCGCGGCGACTGAGGAGGGCGTGAATAGGA
  TCGCAGTGCCAAAACCGCCCTCCATTGAGGAATTCAGCATAGTGAAGCCCATTAGCCGGGGCGCCTTCGG
  GAAAGTGTATCTGGGGCAGAAAGGCGGCAAATTGTATGCAGTAAAGGTTGTTAAAAAAGCAGACATGATC
  AACAAAAATATGACTCATCAGGTCCAAGCTGAGAGAGATGCACTGGCACTAAGCAAAAGCCCATTCATTG
  TCCATTTGTATTATTCACTGCAGTCTGCAAACAATGTCTACTTGGTAATGGAATATCTTATTGGGGGAGA
  TGTCAAGTCTCTCCTACATATATATGGTTATTTTGATGAAGAGATGGCTGTGAAATATATTTCTGAAGTA
  GCACTGGCTCTAGACTACCTTCACAGACATGGAATCATCCACAGGGACTTGAAACCGGACAATATGCTTA
  TTTCTAATGAGGGTCATATTAAACTGACGGATTTTGGCCTTTCAAAAGTTACTTTGAATAGAGATATTAA
  TATGATGGATATCCTTACAACACCATCAATGGCAAAACCTAGACAAGATTATTCAAGAACCCCAGGACAA
  GTGTTATCGCTTATCAGCTCGTTGGGATTTAACACACCAATTGCAGAAAAAAATCAAGACCCTGCAAACA
  TCCTTTCAGCCTGTCTGTCTGAAACATCACAGCTTTCTCAAGGACTCGTATGCCCTATGTCTGTAGATCA
  AAAGGACACTACGCCTTATTCTAGCAAATTACTAAAATCATGTCTTGAAACAGTTGCCTCCAACCCAGGA
  ATGCCTGTGAAGTGTCTAACTTCTAATTTACTCCAGTCTAGGAAAAGGCTGGCCACATCCAGTGCCAGTA
  GTCAATCCCACACCTTCATATCCAGTGTGGAATCAGAATGCCACAGCAGTCCCAAATGGGAAAAAGATTG
  CCAGGAAAGTGATGAAGCATTGGGCCCAACAATGATGAGTTGGAATGCAGTTGAAAAGTTATGCGCAAAA
  TCTGCAAATGCCATTGAGACGAAAGGTTTCAATAAAAAGGATCTGGAGTTAGCTCTTTCTCCCATTCATA
  ACAGCAGTGCCCTTCCCACCACTGGACGCTCTTGTGTAAACCTTGCTAAAAAATGCTTCTCTGGGGAAGT
  TTCTTGGGAAGCAGTAGAACTGGATGTAAATAATATAAATATGGACACTGACACAAGTCAGTTAGGTTTC
  CATCAGTCAAATCAGTGGGCTGTGGATTCTGGTGGGATATCTGAAGAGCACCTTGGGAAAAGAAGTTTAA
  AAAGAAATTTTGAGTTGGTTGACTCCAGTCCTTGTAAAAAAATTATACAGAATAAAAAAACTTGTGTAGA
  GTATAAGCATAACGAAATGACAAATTGTTATACAAATCAAAATACAGGCTTAACAGTTGAAGTGCAGGAC
  CTTAAGCTATCAGTGCACAAAAGTCAACAAAATGACTGTGCTAATAAGGAGAACATTGTCAATTCTTTTA
  CTGATAAACAACAAACACCAGAAAAATTACCTATACCAATGATAGCAAAAAACCTTATGTGTGAACTCGA
  TGAAGACTGTGAAAAGAATAGTAAGAGGGACTACTTAAGTTCTAGTTTTCTATGTTCTGATGATGATAGA
  GCTTCTAAAAATATTTCTATGAACTCTGATTCATCTTTTCCTGGAATTTCTATAATGGAAAGTCCATTAG
  AAAGTCAGCCCTTAGATTCAGATAGAAGCATTAAAGAATCCTCTTTTGAAGAATCAAATATTGAAGATCC
  ACTTATTGTAACACCAGATTGCCAAGAAAAGACCTCACCAAAAGGTGTCGAGAACCCTGCTGTACAAGAG
  AGTAACCAAAAAATGTTAGGTCCTCCTTTGGAGGTGCTGAAAACGTTAGCCTCTAAAAGAAATGCTGTTG
  CTTTTCGAAGTTTTAACAGTCATATTAATGCATCCAATAACTCAGAACCATCCAGAATGAACATGACTTC
  TTTAGATGCAATGGATATTTCGTGTGCCTACAGTGGTTCATATCCCATGGCTATAACCCCTACTCAAAAA
  AGAAGATCCTGTATGCCACATCAGCAGACCCCAAATCAGATCAAGTCGGGAACTCCATACCGAACTCCGA
  AGAGTGTGAGAAGAGGGGTGGCCCCCGTTGATGATGGGCGAATTCTAGGAACCCCAGACTACCTTGCACC
  TGAGCTGTTACTAGGCAGGGCCCATGGTCCTGCGGTAGACTGGTGGGCACTTGGAGTTTGCTTGTTTGAA
  TTTCTAACAGGAATTCCCCCTTTCAATGATGAAACACCACAACAAGTATTCCAGAATATTCTGAAAAGAG
  ATATCCCTTGGCCAGAAGGTGAAGAAAAGTTATCTGATAATGCTCAAAGTGCAGTAGAAATACTTTTAAC
  CATTGATGATACAAAGAGAGCTGGAATGAAAGAGCTAAAACGTCATCCTCTCTTCAGTGATGTGGACTGG
  GAAAATCTGCAGCATCAGACTATGCCTTTCATCCCCCAGCCAGATGATGAAACAGATACCTCCTATTTTG
  AAGCCAGGAATACTGCTCAGCACCTGACCGTATCTGGATTTAGTCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203858 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDPTAGSKKEPGGGAATEEGVNRIAVPKPPSIEEFSIVKPISRGAFGKVYLGQKGGKLYAVKVVKKADMI
  NKNMTHQVQAERDALALSKSPFIVHLYYSLQSANNVYLVMEYLIGGDVKSLLHIYGYFDEEMAVKYISEV
  ALALDYLHRHGIIHRDLKPDNMLISNEGHIKLTDFGLSKVTLNRDINMMDILTTPSMAKPRQDYSRTPGQ
  VLSLISSLGFNTPIAEKNQDPANILSACLSETSQLSQGLVCPMSVDQKDTTPYSSKLLKSCLETVASNPG
  MPVKCLTSNLLQSRKRLATSSASSQSHTFISSVESECHSSPKWEKDCQESDEALGPTMMSWNAVEKLCAK
  SANAIETKGFNKKDLELALSPIHNSSALPTTGRSCVNLAKKCFSGEVSWEAVELDVNNINMDTDTSQLGF
  HQSNQWAVDSGGISEEHLGKRSLKRNFELVDSSPCKKIIQNKKTCVEYKHNEMTNCYTNQNTGLTVEVQD
  LKLSVHKSQQNDCANKENIVNSFTDKQQTPEKLPIPMIAKNLMCELDEDCEKNSKRDYLSSSFLCSDDDR
  ASKNISMNSDSSFPGISIMESPLESQPLDSDRSIKESSFEESNIEDPLIVTPDCQEKTSPKGVENPAVQE
  SNQKMLGPPLEVLKTLASKRNAVAFRSFNSHINASNNSEPSRMNMTSLDAMDISCAYSGSYPMAITPTQK
  RRSCMPHQQTPNQIKSGTPYRTPKSVRRGVAPVDDGRILGTPDYLAPELLLGRAHGPAVDWWALGVCLFE
  FLTGIPPFNDETPQQVFQNILKRDIPWPEGEEKLSDNAQSAVEILLTIDDTKRAGMKELKRHPLFSDVDW
  ENLQHQTMPFIPQPDDETDTSYFEARNTAQHLTVSGFSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032844
• ORF Size: 2637 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032844.5
• RefSeq Size: 3623 bp
• RefSeq ORF: 2637 bp
• Locus ID: 84930
• UniProt ID: Q96GX5
• Cytogenetics: 10p12.1
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• MW: 97.3 kDa
• Gene Summary: This gene encodes a microtubule-associated serine/threonine kinase. Mutations at this locus have been associated with autosomal dominant thrombocytopenia, also known as thrombocytopenia-2. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Feb 2010]