TMEM169 (NM_138390) Human Tagged ORF Clone

CAT#: RC203846

TMEM169 (Myc-DDK-tagged)-Human transmembrane protein 169 (TMEM169), transcript variant 3

Product Images

Circular map for RC203846

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  Western blot validation of overexpression lysate (Cat# LY408654) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203846 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TMEM169 protein (Cat# TP303846). The protein was produced from HEK293T cells transfected with TMEM169 cDNA clone (Cat# RC203846) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TMEM169
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203846 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAGCCAACAGCAGTAGAAGGCCAGGTCCAGCTTCCAAGCCCCCACCAGGGCTCTCTCAGGAAGG
  CTGTGGCTGCTGCCCTGGCGCTGGATGGGGAATCCACAATGGGGCACAGGAAAAAGAAGAGGAAAGAGTC
  ACGCCCAGAATCCATCATCATCTACCGCTCAGACAATGAGAAAACAGATGAGGAGCCCGGAGAATCAGAA
  GGTGGAGATCAGCCTAAAGAGGAGGAGGGAGATGATTTCCTAGACTATCCTGTGGATGATGATATGTGGA
  ACCTGCCTCTGGACAGCCGCTACGTCACCTTAACTGGGACCATCACACGAGGGAAGAAAAAGGGTCAGAT
  GGTGGACATCCATGTCACATTGACAGAGAAAGAGCTGCAGGAACTGACCAAACCTAAAGAGTCATCAAGG
  GAAACGACGCCTGAAGGAAGAATGGCCTGCCAGATGGGAGCTGACCGTGGGCCCCATGTGGTCCTCTGGA
  CGCTGATCTGCCTGCCTGTGGTTTTCATCCTTTCTTTTGTTGTCTCTTTCTACTACGGCACTATCACCTG
  GTACAACATCTTCCTCGTGTATAATGAGGAAAGGACCTTCTGGCACAAGATCTCGTATTGCCCTTGCCTC
  GTTCTCTTCTATCCAGTGCTCATCATGGCCATGGCTTCTTCCCTCGGCCTCTACGCTGCTGTGGTCCAGC
  TCTCGTGGTCCTGGGAAGCATGGTGGCAAGCTGCCCGGGACATGGAGAAAGGCTTCTGTGGCTGGCTCTG
  CAGCAAGCTGGGTCTGGAGGACTGTTCTCCCTACAGCATTGTGGAGTTGCTTGAATCCGACAATATCTCA
  AGCACTCTCTCCAACAAGGACCCCATCCAAGAAGTAGAAACCTCCACGGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203846 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEEPTAVEGQVQLPSPHQGSLRKAVAAALALDGESTMGHRKKKRKESRPESIIIYRSDNEKTDEEPGESE
  GGDQPKEEEGDDFLDYPVDDDMWNLPLDSRYVTLTGTITRGKKKGQMVDIHVTLTEKELQELTKPKESSR
  ETTPEGRMACQMGADRGPHVVLWTLICLPVVFILSFVVSFYYGTITWYNIFLVYNEERTFWHKISYCPCL
  VLFYPVLIMAMASSLGLYAAVVQLSWSWEAWWQAARDMEKGFCGWLCSKLGLEDCSPYSIVELLESDNIS
  STLSNKDPIQEVETSTV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138390
• ORF Size: 891 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138390.4
• RefSeq Size: 3387 bp
• RefSeq ORF: 894 bp
• Locus ID: 92691
• UniProt ID: Q96HH4
• Cytogenetics: 2q35
• Protein Families: Transmembrane
• MW: 33.6 kDa