ACTR3B (NM_001040135) Human Tagged ORF Clone

CAT#: RC203790

ACTR3B (Myc-DDK-tagged)-Human ARP3 actin-related protein 3 homolog B (yeast) (ACTR3B), transcript variant 2

Product Images

Circular map for RC203790

• 

  Western blot validation of overexpression lysate (Cat# LY421689) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203790 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ACTR3B
• Synonyms: ARP3BETA; ARP11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203790 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGCTCCCTGCCTCCCTGCGTGGTGGACTGTGGCACCGGGTATACCAAGCTTGGCTACGCAGGCA
  ACACTGAGCCCCAGTTCATTATTCCTTCATGTATTGCCATCAGAGAGTCAGCAAAGGTAGTTGACCAAGC
  TCAAAGGAGAGTGTTGAGGGGAGTTGATGACCTTGACTTTTTCATAGGAGATGAAGCCATCGATAAACCT
  ACATATGCTACAAAGTGGCCGATACGACATGGAATCATTGAAGACTGGGATCTTATGGAAAGGTTCATGG
  AGCAAGTGGTTTTTAAATATCTTCGAGCTGAACCTGAGGACCATTATTTTTTAATGACAGAACCTCCACT
  CAATACACCAGAAAACAGAGAGTATCTTGCAGAAATTATGTTTGAATCATTTAACGTACCAGGACTCTAC
  ATTGCAGTTCAGGCAGTGCTGGCCTTGGCGGCATCTTGGACATCTCGACAAGTGGGTGAACGTACGTTAA
  CGGGGATAGTCATTGACAGCGGAGATGGAGTCACCCATGTTATCCCAGTGGCAGAAGGTTATGTAATTGG
  AAGCTGCATCAAACACATCCCGATTGCAGGTAGAGATATTACGTATTTCATTCAACAGCTGCTAAGGGAG
  AGGGAGGTGGGAATCCCTCCTGAGCAGTCACTGGAGACCGCAAAAGCCATTAAGGAGAAATACTGTTACA
  TTTGCCCCGATATAGTCAAGGAATTTGCCAAGTATGATGTGGATCCCCGGAAGTGGATCAAACAGTACAC
  GGGTATCAATGCGATCAACCAGAAGAAGTTTGTTATAGACGTTGGTTACGAAAGATTCCTGGGACCTGAA
  ATATTCTTTCACCCGGAGTTTGCCAACCCAGACTTTATGGAGTCCATCTCAGATGTTGTTGATGAAGTAA
  TACAGAACTGCCCCATCGATGTGCGGCGCCCGCTGTATAAGCCCGAGTTCTTTCAGGTCTGCCACACCAA
  GAAGGACTATGAAGAGTACGGGCCCAGCATCTGCCGCCACAACCCCGTCTTTGGAGTCATGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203790 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGSLPPCVVDCGTGYTKLGYAGNTEPQFIIPSCIAIRESAKVVDQAQRRVLRGVDDLDFFIGDEAIDKP
  TYATKWPIRHGIIEDWDLMERFMEQVVFKYLRAEPEDHYFLMTEPPLNTPENREYLAEIMFESFNVPGLY
  IAVQAVLALAASWTSRQVGERTLTGIVIDSGDGVTHVIPVAEGYVIGSCIKHIPIAGRDITYFIQQLLRE
  REVGIPPEQSLETAKAIKEKYCYICPDIVKEFAKYDVDPRKWIKQYTGINAINQKKFVIDVGYERFLGPE
  IFFHPEFANPDFMESISDVVDEVIQNCPIDVRRPLYKPEFFQVCHTKKDYEEYGPSICRHNPVFGVMS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001040135
• ORF Size: 1044 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001040135.2
• RefSeq Size: 2013 bp
• RefSeq ORF: 1047 bp
• Locus ID: 57180
• UniProt ID: Q9P1U1
• Cytogenetics: 7q36.1-q36.2
• MW: 39.7 kDa
• Gene Summary: This gene encodes a member of the actin-related proteins (ARP), which form multiprotein complexes and share 35-55% amino acid identity with conventional actin. The protein encoded by this gene may have a regulatory role in the actin cytoskeleton and induce cell-shape change and motility. Pseudogenes of this gene are located on chromosomes 2, 4, 10, 16, 22 and Y. Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Jul 2012]