Lipoprotein lipase (LPL) (NM_000237) Human Tagged ORF Clone

CAT#: RC203766

LPL (Myc-DDK-tagged)-Human lipoprotein lipase (LPL)

Product Images

Circular map for RC203766

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY LPL (Cat# RC203766, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LPL (Cat# TA503793). Positive lysates LY400089 (100ug) and LC400089 (20ug) can be purchased separately from OriGene.

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  Coomassie blue staining of purified LPL protein (Cat# TP303766). The protein was produced from HEK293T cells transfected with LPL cDNA clone (Cat# RC203766) using MegaTran 2.0 (Cat# TT210002).

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  HEK293T cells transfected with either RC203766 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LPL antibody (TA503793), and then analyzed by flow cytometry.

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lipoprotein lipase
• Synonyms: HDLCQ11; LIPD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203766 representing NM_000237
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGCAAAGCCCTGCTCGTGCTGACTCTGGCCGTGTGGCTCCAGAGTCTGACCGCCTCCCGCGGAG
  GGGTGGCCGCCGCCGACCAAAGAAGAGATTTTATCGACATCGAAAGTAAATTTGCCCTAAGGACCCCTGA
  AGACACAGCTGAGGACACTTGCCACCTCATTCCCGGAGTAGCAGAGTCCGTGGCTACCTGTCATTTCAAT
  CACAGCAGCAAAACCTTCATGGTGATCCATGGCTGGACGGTAACAGGAATGTATGAGAGTTGGGTGCCAA
  AACTTGTGGCCGCCCTGTACAAGAGAGAACCAGACTCCAATGTCATTGTGGTGGACTGGCTGTCACGGGC
  TCAGGAGCATTACCCAGTGTCCGCGGGCTACACCAAACTGGTGGGACAGGATGTGGCCCGGTTTATCAAC
  TGGATGGAGGAGGAGTTTAACTACCCTCTGGACAATGTCCATCTCTTGGGATACAGCCTTGGAGCCCATG
  CTGCTGGCATTGCAGGAAGTCTGACCAATAAGAAAGTCAACAGAATTACTGGCCTCGATCCAGCTGGACC
  TAACTTTGAGTATGCAGAAGCCCCGAGTCGTCTTTCTCCTGATGATGCAGATTTTGTAGACGTCTTACAC
  ACATTCACCAGAGGGTCCCCTGGTCGAAGCATTGGAATCCAGAAACCAGTTGGGCATGTTGACATTTACC
  CGAATGGAGGTACTTTTCAGCCAGGATGTAACATTGGAGAAGCTATCCGCGTGATTGCAGAGAGAGGACT
  TGGAGATGTGGACCAGCTAGTGAAGTGCTCCCACGAGCGCTCCATTCATCTCTTCATCGACTCTCTGTTG
  AATGAAGAAAATCCAAGTAAGGCCTACAGGTGCAGTTCCAAGGAAGCCTTTGAGAAAGGGCTCTGCTTGA
  GTTGTAGAAAGAACCGCTGCAACAATCTGGGCTATGAGATCAATAAAGTCAGAGCCAAAAGAAGCAGCAA
  AATGTACCTGAAGACTCGTTCTCAGATGCCCTACAAAGTCTTCCATTACCAAGTAAAGATTCATTTTTCT
  GGGACTGAGAGTGAAACCCATACCAATCAGGCCTTTGAGATTTCTCTGTATGGCACCGTGGCCGAGAGTG
  AGAACATCCCATTCACTCTGCCTGAAGTTTCCACAAATAAGACCTACTCCTTCCTAATTTACACAGAGGT
  AGATATTGGAGAACTACTCATGTTGAAGCTCAAATGGAAGAGTGATTCATACTTTAGCTGGTCAGACTGG
  TGGAGCAGTCCCGGCTTCGCCATTCAGAAGATCAGAGTAAAAGCAGGAGAGACTCAGAAAAAGGTGATCT
  TCTGTTCTAGGGAGAAAGTGTCTCATTTGCAGAAAGGAAAGGCACCTGCGGTATTTGTGAAATGCCATGA
  CAAGTCTCTGAATAAGAAGTCAGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203766 representing NM_000237
  Red=Cloning site Green=Tags(s)
  
  MESKALLVLTLAVWLQSLTASRGGVAAADQRRDFIDIESKFALRTPEDTAEDTCHLIPGVAESVATCHFN
  HSSKTFMVIHGWTVTGMYESWVPKLVAALYKREPDSNVIVVDWLSRAQEHYPVSAGYTKLVGQDVARFIN
  WMEEEFNYPLDNVHLLGYSLGAHAAGIAGSLTNKKVNRITGLDPAGPNFEYAEAPSRLSPDDADFVDVLH
  TFTRGSPGRSIGIQKPVGHVDIYPNGGTFQPGCNIGEAIRVIAERGLGDVDQLVKCSHERSIHLFIDSLL
  NEENPSKAYRCSSKEAFEKGLCLSCRKNRCNNLGYEINKVRAKRSSKMYLKTRSQMPYKVFHYQVKIHFS
  GTESETHTNQAFEISLYGTVAESENIPFTLPEVSTNKTYSFLIYTEVDIGELLMLKLKWKSDSYFSWSDW
  WSSPGFAIQKIRVKAGETQKKVIFCSREKVSHLQKGKAPAVFVKCHDKSLNKKSG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000237
• ORF Size: 1425 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000237.3
• RefSeq Size: 3747 bp
• RefSeq ORF: 1428 bp
• Locus ID: 4023
• UniProt ID: P06858
• Cytogenetics: 8p21.3
• Domains: lipase, PLAT
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Glycerolipid metabolism, PPAR signaling pathway
• MW: 53.2 kDa
• Gene Summary: LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism. [provided by RefSeq, Jul 2008]