Gemin 4 (GEMIN4) (NM_015721) Human Tagged ORF Clone

CAT#: RC203733

GEMIN4 (Myc-DDK-tagged)-Human gem (nuclear organelle) associated protein 4 (GEMIN4)

Product Images

Circular map for RC203733

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gemin 4
• Synonyms: HC56; HCAP1; HHRF-1; NEDMCR; p97
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203733 representing NM_015721
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCTAGGACCCTTGAACATCTGTGAAGAAATGACTATTCTGCATGGAGGCTTCTTGCTGGCCGAGC
  AGCTGTTCCACCCTAAGGCACTGGCAGAATTAACAAAGTCTGACTGGGAACGTGTTGGACGGCCCATCGT
  GGAGGCCTTAAGGGAGATCTCCTCGGCTGCAGCACACTCCCAGCCCTTTGCCTGGAAGAAGAAAGCCCTG
  ATCATCATCTGGGCCAAGGTTCTGCAGCCGCACCCCGTGACCCCGTCCGACACAGAGACACGGTGGCAGG
  AAGACCTGTTCTTCTCGGTGGGCAACATGATCCCCACCATCAACCACACCATCCTCTTCGAGCTGCTCAA
  ATCCCTGGAAGCTTCTGGACTCTTTATCCAGCTCCTGATGGCCCTGCCCACCACCATCTGCCATGCAGAA
  CTAGAGCGCTTTCTGGAACATGTGACCGTTGACACTTCTGCCGAAGACGTGGCCTTCTTCCTGGACGTCT
  GGTGGGAGGTGATGAAGCACAAGGGTCACCCGCAGGACCCCCTGCTCTCCCAGTTTAGTGCAATGGCCCA
  TAAGTACCTGCCTGCCTTAGATGAGTTCCCCCATCCTCCAAAGAGGCTTAGGTCAGACCCAGACGCGTGC
  CCCACCATGCCCCTGTTGGCCATGCTGCTCCGCGGGCTGACACAGATCCAGAGTCGGATCCTGGGCCCGG
  GGAGGAAGTGCTGTGCGCTGGCCAACCTGGCTGACATGCTGACTGTGTTTGCGCTGACAGAGGACGACCC
  CCAGGAGGTGTCTGCAACCGTGTATCTGGACAAACTGGCCACGGTGATCTCTGTGTGGAACTCGGACACC
  CAGAATCCCTACCACCAGCAGGCGCTGGCAGAGAAGGTGAAGGAGGCAGAACGGGATGTCAGCCTGACCT
  CGCTGGCCAAACTCCCCAGTGAGACCATTTTCGTGGGCTGCGAGTTCCTGCACCACCTGCTGCGGGAGTG
  GGGGGAGGAGTTGCAGGCCGTGCTCCGCAGCAGCCAGGGGACAAGTTACGACAGCTACCGGCTGTGCGAC
  AGTCTGACTTCCTTCAGCCAGAACGCGACGCTCTACCTGAACCGCACCAGCCTGTCCAAGGAGGACAGGC
  AGGTGGTCTCTGAGCTGGCGGAGTGTGTCAGGGACTTCCTGAGGAAAACGAGCACGGTGCTGAAGAACAG
  GGCCTTGGAGGATATCACAGCTTCCATTGCCATGGCCGTCATCCAGCAGAAGATGGACCGCCATATGGAA
  GTGTGCTACATTTTTGCCTCTGAGAAGAAGTGGGCCTTCTCGGACGAGTGGGTAGCCTGCCTGGGGAGTA
  ACAGGGCCCTCTTCCGACAGCCAGACTTGGTGTTGAGGCTGCTGGAAACAGTGATAGACGTCAGCACAGC
  TGACAGAGCCATCCCTGAGTCTCAGATCCGGCAGGTGATCCACCTGATCCTGGAATGTTACGCAGACCTC
  TCCCTGCCAGGTAAAAATAAAGTCCTTGCAGGTATCCTGCGTTCCTGGGGGCGAAAGGGCCTCTCTGAAA
  AGTTGCTGGCTTATGTGGAGGGTTTTCAGGAAGACCTCAATACAACTTTTAACCAGCTCACTCAGAGTGC
  CTCCGAACAGGGCTTGGCAAAAGCTGTGGCCTCCGTGGCCCGCCTGGTCATAGTGCACCCGGAAGTCACG
  GTGAAGAAAATGTGCAGCCTGGCTGTGGTCAATCTCGGCACCCACAAGTTCCTGGCCCAGATTCTCACTG
  CCTTCCCTGCCCTTAGGTTTGTGGAAGAGCAGGGTCCCAATTCATCTGCCACTTTCATGGTGTCATGCCT
  CAAAGAAACCGTCTGGATGAAGTTCTCTACACCCAAGGAAGAAAAGCAATTTTTAGAGCTCCTGAACTGC
  CTGATGAGTCCCGTGAAACCCCAAGGGATTCCAGTGGCTGCTCTTCTTGAGCCAGACGAGGTGCTGAAGG
  AATTTGTCCTGCCTTTCTTGAGGTTAGATGTTGAAGAGGTAGACCTCAGTCTGAGGATCTTCATCCAGAC
  TCTAGAGGCAAACGCGTGCCGAGAGGAATACTGGCTCCAGACCTGCTCCCCGTTTCCACTCCTCTTCAGC
  TTGTGCCAGCTCTTGGACCGCTTCAGCAAATACTGGCAGCTTCCCAAGGAGAAGCGGTGCCTCTCTTTGG
  ATAGGAAGGATCTAGCGATCCATATCCTGGAGCTCCTGTGTGAGATTGTATCAGCCAATGCTGAGACCTT
  CTCCCCGGATGTCTGGATCAAGTCCCTGTCCTGGCTCCACCGCAAGTTAGAACAGCTAGACTGGACTGTG
  GGCCTGAGGCTGAAGAGCTTCTTCGAGGGGCACTTCAAGTGTGAAGTGCCAGCCACACTTTTTGAGATCT
  GTAAGCTTTCAGAAGACGAGTGGACCTCCCAGGCCCACCCAGGGTACGGGGCTGGCACGGGGCTCCTGGC
  CTGGATGGAGTGCTGCTGCGTCTCCAGCGGCATCTCGGAGAGGATGCTGTCTCTCTTGGTGGTGGACGTG
  GGCAATCCTGAGGAGGTCAGACTGTTCAGCAAAGGCTTTCTGGTGGCCCTGGTGCAAGTCATGCCTTGGT
  GCAGCCCTCAGGAGTGGCAGCGCCTTCACCAGCTGACCAGGAGACTGCTGGAGAAGCAGCTCCTCCATGT
  CCCTTATAGCCTGGAATATATTCAGTTTGTTCCCCTGCTCAACCTGAAGCCCTTTGCCCAGGAGTTGCAA
  CTCTCCGTCCTCTTCCTGAGGACTTTCCAGTTTCTCTGCAGCCATAGCTGTCGTGATTGGCTTCCTCTGG
  AAGGCTGGAACCACGTGGTCAAACTCCTCTGTGGCAGTCTGACCCGCCTCCTGGACTCAGTCAGGGCGAT
  ACAGGCAGCTGGCCCTTGGGTTCAAGGACCAGAGCAGGACCTGACCCAGGAAGCCCTGTTTGTTTACACC
  CAGGTGTTCTGCCATGCTCTGCACATCATGGCCATGCTCCACCCGGAGGTCTGTGAGCCACTCTACGTTT
  TAGCCTTGGAAACCCTCACCTGCTATGAGACTTTGAGCAAGACCAACCCTTCTGTCAGCTCCTTGCTCCA
  GAGGGCACACGAGCAGCGCTTCTTAAAGTCCATTGCTGAGGGCATCGGCCCTGAAGAACGGCGCCAAACC
  CTGTTGCAGAAGATGAGCAGCTTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203733 representing NM_015721
  Red=Cloning site Green=Tags(s)
  
  MDLGPLNICEEMTILHGGFLLAEQLFHPKALAELTKSDWERVGRPIVEALREISSAAAHSQPFAWKKKAL
  IIIWAKVLQPHPVTPSDTETRWQEDLFFSVGNMIPTINHTILFELLKSLEASGLFIQLLMALPTTICHAE
  LERFLEHVTVDTSAEDVAFFLDVWWEVMKHKGHPQDPLLSQFSAMAHKYLPALDEFPHPPKRLRSDPDAC
  PTMPLLAMLLRGLTQIQSRILGPGRKCCALANLADMLTVFALTEDDPQEVSATVYLDKLATVISVWNSDT
  QNPYHQQALAEKVKEAERDVSLTSLAKLPSETIFVGCEFLHHLLREWGEELQAVLRSSQGTSYDSYRLCD
  SLTSFSQNATLYLNRTSLSKEDRQVVSELAECVRDFLRKTSTVLKNRALEDITASIAMAVIQQKMDRHME
  VCYIFASEKKWAFSDEWVACLGSNRALFRQPDLVLRLLETVIDVSTADRAIPESQIRQVIHLILECYADL
  SLPGKNKVLAGILRSWGRKGLSEKLLAYVEGFQEDLNTTFNQLTQSASEQGLAKAVASVARLVIVHPEVT
  VKKMCSLAVVNLGTHKFLAQILTAFPALRFVEEQGPNSSATFMVSCLKETVWMKFSTPKEEKQFLELLNC
  LMSPVKPQGIPVAALLEPDEVLKEFVLPFLRLDVEEVDLSLRIFIQTLEANACREEYWLQTCSPFPLLFS
  LCQLLDRFSKYWQLPKEKRCLSLDRKDLAIHILELLCEIVSANAETFSPDVWIKSLSWLHRKLEQLDWTV
  GLRLKSFFEGHFKCEVPATLFEICKLSEDEWTSQAHPGYGAGTGLLAWMECCCVSSGISERMLSLLVVDV
  GNPEEVRLFSKGFLVALVQVMPWCSPQEWQRLHQLTRRLLEKQLLHVPYSLEYIQFVPLLNLKPFAQELQ
  LSVLFLRTFQFLCSHSCRDWLPLEGWNHVVKLLCGSLTRLLDSVRAIQAAGPWVQGPEQDLTQEALFVYT
  QVFCHALHIMAMLHPEVCEPLYVLALETLTCYETLSKTNPSVSSLLQRAHEQRFLKSIAEGIGPEERRQT
  LLQKMSSF
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015721
• ORF Size: 3174 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015721.3
• RefSeq Size: 3757 bp
• RefSeq ORF: 3177 bp
• Locus ID: 50628
• UniProt ID: P57678
• Cytogenetics: 17p13.3
• Protein Families: Druggable Genome
• MW: 120.5 kDa
• Gene Summary: The product of this gene is part of a large complex localized to the cytoplasm, nucleoli, and to discrete nuclear bodies called Gemini bodies (gems). The complex functions in spliceosomal snRNP assembly in the cytoplasm, and regenerates spliceosomes required for pre-mRNA splicing in the nucleus. The encoded protein directly interacts with a DEAD box protein and several spliceosome core proteins. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]