CENTB1 (ACAP1) (NM_014716) Human Tagged ORF Clone

CAT#: RC203724

ACAP1 (Myc-DDK-tagged)-Human ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 (ACAP1)

Product Images

Circular map for RC203724

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  Western blot validation of overexpression lysate (Cat# LY415090) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203724 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ACAP1 protein (Cat# TP303724). The protein was produced from HEK293T cells transfected with ACAP1 cDNA clone (Cat# RC203724) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CENTB1
• Synonyms: CENTB1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203724 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGTCAAGCTGGATTTCGAGGAGTGTCTCAAGGACTCACCCCGTTTCCGAGCCTCTATTGAGCTGG
  TGGAAGCCGAAGTGTCAGAATTGGAGACCCGTCTGGAAAAGCTCCTGAAACTGGGCACTGGTCTCCTGGA
  AAGTGGGCGCCATTACCTTGCTGCCAGCCGCGCCTTCGTTGTCGGCATTTGTGACCTGGCCCGCCTGGGT
  CCACCAGAGCCCATGATGGCGGAGTGTCTGGAAAAATTCACCGTGAGCCTGAACCACAAGCTGGACAGCC
  ATGCGGAGCTTCTAGATGCCACCCAACACACACTGCAGCAGCAGATCCAGACCCTGGTCAAGGAAGGTCT
  GCGGGGTTTCCGAGAGGCTCGCCGGGATTTCTGGCGGGGGGCTGAGAGCCTGGAGGCTGCCCTGACCCAC
  AACGCAGAGGTTCCCAGGCGCCGGGCCCAGGAGGCAGAAGAGGCAGGAGCTGCTTTGAGGACGGCTCGAG
  CTGGGTACCGGGGACGGGCACTGGATTATGCCCTGCAGATCAACGTGATTGAGGACAAGAGGAAGTTTGA
  CATCATGGAGTTTGTGCTGCGTTTGGTGGAGGCCCAGGCTACCCATTTCCAGCAGGGCCATGAGGAGCTG
  AGCCGGCTGTCCCAGTATCGAAAGGAGCTGGGCGCCCAGTTGCACCAGCTGGTCTTGAATTCAGCACGAG
  AGAAGAGGGACATGGAGCAGAGACACGTGCTGCTGAAACAGAAGGAGCTGGGTGGGGAGGAGCCAGAACC
  AAGCTTAAGAGAGGGGCCTGGTGGCCTGGTGATGGAAGGACATCTCTTCAAACGGGCCAGCAACGCATTT
  AAGACCTGGAGCAGACGCTGGTTCACCATTCAGAGCAACCAACTGGTTTACCAGAAGAAGTACAAGGACC
  CTGTGACTGTGGTGGTGGATGACCTTCGTCTCTGCACAGTGAAACTCTGCCCTGACTCAGAAAGGCGGTT
  CTGCTTTGAGGTGGTGTCCACCAGCAAGTCCTGCCTCCTCCAGGCTGACTCAGAGCGCCTCCTGCAGCTG
  TGGGTCAGTGCTGTGCAGAGCAGCATTGCTTCTGCCTTCAGTCAGGCTCGCCTTGATGACAGCCCCCGGG
  GTCCAGGCCAGGGCTCAGGACACCTGGCCATAGGCTCTGCTGCCACCCTGGGCTCTGGTGGAATGGCCAG
  GGGAAGGGAGCCTGGGGGAGTCGGGCACGTGGTGGCCCAGGTCCAGAGTGTGGATGGCAATGCCCAGTGC
  TGCGACTGCCGGGAGCCAGCCCCGGAGTGGGCCAGCATCAACCTTGGTGTCACCCTCTGCATTCAGTGTT
  CCGGCATCCACAGGAGCCTTGGTGTTCACTTCTCCAAAGTCCGGTCTCTGACCCTTGACTCATGGGAGCC
  AGAACTAGTGAAGCTCATGTGTGAGCTGGGAAATGTCATCATCAACCAGATCTATGAGGCCCGCGTGGAG
  GCCATGGCAGTGAAGAAACCAGGGCCCAGCTGCTCCCGGCAGGAGAAGGAGGCCTGGATTCACGCTAAAT
  ACGTGGAGAAGAAGTTCCTGACCAAGCTGCCTGAGATTCGAGGGCGAAGAGGTGGCCGGGGGCGCCCAAG
  GGGGCAGCCTCCTGTGCCCCCAAAGCCTTCCATCAGGCCCCGGCCAGGGAGCTTGAGATCCAAGCCAGAG
  CCCCCCTCTGAGGACCTGGGAAGCCTGCACCCTGGGGCCCTACTGTTTCGAGCGTCTGGGCATCCTCCAT
  CTCTTCCCACCATGGCTGATGCCCTTGCCCATGGAGCTGATGTCAACTGGGTCAATGGGGGCCAAGATAA
  TGCCACACCGCTGATCCAGGCCACAGCTGCTAATTCTCTTCTGGCCTGTGAGTTTCTCCTCCAGAACGGG
  GCGAACGTGAACCAAGCGGACAGTGCGGGCCGGGGCCCGCTGCACCACGCAACCATTCTTGGCCACACGG
  GGCTCGCCTGCCTGTTCCTGAAACGGGGAGCTGATCTGGGGGCTCGAGACTCTGAAGGCAGGGACCCTCT
  GACCATCGCCATGGAAACAGCCAACGCTGACATCGTCACCCTGCTACGACTGGCAAAGATGAGGGAGGCT
  GAAGCGGCCCAGGGGCAGGCAGGAGATGAGACGTATCTTGACATCTTCCGCGACTTCTCCCTCATGGCGT
  CAGACGACCCGGAGAAGCTGAGCCGTCGCAGTCATGACCTCCACACGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203724 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTVKLDFEECLKDSPRFRASIELVEAEVSELETRLEKLLKLGTGLLESGRHYLAASRAFVVGICDLARLG
  PPEPMMAECLEKFTVSLNHKLDSHAELLDATQHTLQQQIQTLVKEGLRGFREARRDFWRGAESLEAALTH
  NAEVPRRRAQEAEEAGAALRTARAGYRGRALDYALQINVIEDKRKFDIMEFVLRLVEAQATHFQQGHEEL
  SRLSQYRKELGAQLHQLVLNSAREKRDMEQRHVLLKQKELGGEEPEPSLREGPGGLVMEGHLFKRASNAF
  KTWSRRWFTIQSNQLVYQKKYKDPVTVVVDDLRLCTVKLCPDSERRFCFEVVSTSKSCLLQADSERLLQL
  WVSAVQSSIASAFSQARLDDSPRGPGQGSGHLAIGSAATLGSGGMARGREPGGVGHVVAQVQSVDGNAQC
  CDCREPAPEWASINLGVTLCIQCSGIHRSLGVHFSKVRSLTLDSWEPELVKLMCELGNVIINQIYEARVE
  AMAVKKPGPSCSRQEKEAWIHAKYVEKKFLTKLPEIRGRRGGRGRPRGQPPVPPKPSIRPRPGSLRSKPE
  PPSEDLGSLHPGALLFRASGHPPSLPTMADALAHGADVNWVNGGQDNATPLIQATAANSLLACEFLLQNG
  ANVNQADSAGRGPLHHATILGHTGLACLFLKRGADLGARDSEGRDPLTIAMETANADIVTLLRLAKMREA
  EAAQGQAGDETYLDIFRDFSLMASDDPEKLSRRSHDLHTL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014716
• ORF Size: 2220 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014716.4
• RefSeq Size: 2523 bp
• RefSeq ORF: 2223 bp
• Locus ID: 9744
• UniProt ID: Q15027
• Cytogenetics: 17p13.1
• Domains: ArfGap, PH, ANK
• Protein Pathways: Endocytosis
• MW: 81.5 kDa
• Gene Summary: GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.[UniProtKB/Swiss-Prot Function]