KIAA0408 (NM_014702) Human Tagged ORF Clone

CAT#: RC203723

KIAA0408 (Myc-DDK-tagged)-Human KIAA0408 (KIAA0408)

Product Images

Circular map for RC203723

• 

  Western blot validation of overexpression lysate (Cat# LY415080) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203723 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KIAA0408
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203723 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCTACATAAGCAGTGGGAGAACACAGAGACTAACTGGCATAAGGAAAAGATGGAATTACTGGACC
  AGTTTGACAATGAAAGAAAGGAATGGGAAAGTCAATGGAAGATTATGCAGAAGAAAATAGAAGAGCTTTG
  CCGGGAAGTAAAGCTTTGGAGGAAAATCAATATCAATGAACGTGCTAAGATCATTGATCTTTACCATGAG
  AAGACCATTCCAGAGAAAGTGATAGAATCTTCCCCAAATTACCCCGATTTAGGACAAAGTGAATTTATAA
  GGACGAATCACAAAGATGGTCTGAGAAAAGAAAATAAAAGAGAGCAGAGCTTAGTCAGTGGAGGAAATCA
  AATGTGTAAGGAACAAAAAGCAACAAAAAAATCAAAAGTAGGGTTTTTGGATCCTTTGGCTACAGACAAC
  CAAAAGGAATGTGAGGCCTGGCCTGACCTGAGGACTTCTGAGGAAGACAGCAAGAGCTGTTCTGGCGCCC
  TCAGTACAGCTCTTGAAGAACTTGCGAAGGTGAGTGAAGAATTATGCAGCTTTCAAGAGGAAATTCGAAA
  GCGGTCTAACCATAGAAGGATGAAGTCAGATTCTTTTCTCCAGGAAATGCCAAATGTAACTAATATACCT
  CATGGGGACCCCATGATCAACAATGACCAGTGCATTCTTCCAATCAGTTTAGAAAAAGAAAAACAGAAAA
  ATAGGAAGAATCTGAGCTGTACCAATGTGCTCCAGAGCAATTCTACGAAAAAATGTGGAATTGATACAAT
  CGATTTAAAAAGAAATGAAACTCCACCAGTTCCTCCTCCAAGAAGCACCTCTCGAAATTTTCCCAGCTCG
  GATTCTGAACAAGCCTATGAAAGATGGAAGGAAAGGTTAGACCACAACAGCTGGGTGCCCCATGAGGGTC
  GAAGTAAAAGGAATTACAACCCTCACTTCCCTTTGAGACAACAAGAGATGTCTATGTTGTATCCAAATGA
  AGGGAAAACTTCGAAAGATGGTATCATCTTTTCCTCTTTGGTACCAGAAGTCAAAATAGATAGCAAGCCT
  CCAAGTAATGAAGATGTTGGACTTAGCATGTGGTCATGTGACATTGGGATAGGTGCAAAAAGGAGCCCCT
  CTACTTCGTGGTTTCAGAAAACCTGCTCTACCCCCAGTAATCCAAAATATGAAATGGTGATCCCAGATCA
  CCCTGCTAAATCTCATCCTGATCTTCATGTAAGTAATGACTGTAGCTCCTCAGTAGCAGAGAGCAGTAGC
  CCACTTAGAAATTTCAGTTGTGGCTTTGAAAGGACTACAAGGAATGAGAAGCTGGCAGCAAAGACTGATG
  AATTTAACAGAACTGTATTTAGAACAGATAGAAATTGTCAGGCAATACAGCAAAATCACAGCTGCTCAAA
  ATCATCGGAGGATCTCAAGCCCTGTGATACCTCATCTACTCACACAGGTAGCATATCACAAAGTAACGAT
  GTGTCCGGTATTTGGAAAACCAATGCCCACATGCCTGTGCCCATGGAAAATGTGCCTGATAATCCCACCA
  AGAAATCCACAACAGGCCTAGTAAGACAAATGCAGGGACACCTAAGTCCTCGCAGTTATCGAAATATGCT
  CCACGAGCATGACTGGAGACCGAGTAATTTGTCTGGCCGTCCGAGGTCAGCTGATCCCAGGTCAAATTAT
  GGTGTTGTGGAAAAGCTGCTGAAAACCTATGAGACAGCAACAGAGTCTGCATTGCAAAATTCTAAGTGCT
  TCCAGGATAATTGGACCAAATGTAATTCTGATGTCAGTGGTGGTGCCACATTAAGTCAGCATTTAGAAAT
  GCTCCAAATGGAACAACAGTTTCAGCAAAAGACAGCTGTGTGGGGGGGACAGGAAGTGAAGCAAGGAATA
  GATCCGAAAAAGATAACAGAGGAATCCATGTCAGTGAACGCCTCACATGGAAAAGGATTTTCCCGACCTG
  CTAGACCAGCAAATCGTCGTCTCCCCTCCAGATGGGCATCCAGATCTCCATCTGCACCCCCTGCCTTGCG
  GAGAACTACCCACAACTATACCATTTCTCTGCGATCCGAAGCATTGATGGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203723 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDLHKQWENTETNWHKEKMELLDQFDNERKEWESQWKIMQKKIEELCREVKLWRKININERAKIIDLYHE
  KTIPEKVIESSPNYPDLGQSEFIRTNHKDGLRKENKREQSLVSGGNQMCKEQKATKKSKVGFLDPLATDN
  QKECEAWPDLRTSEEDSKSCSGALSTALEELAKVSEELCSFQEEIRKRSNHRRMKSDSFLQEMPNVTNIP
  HGDPMINNDQCILPISLEKEKQKNRKNLSCTNVLQSNSTKKCGIDTIDLKRNETPPVPPPRSTSRNFPSS
  DSEQAYERWKERLDHNSWVPHEGRSKRNYNPHFPLRQQEMSMLYPNEGKTSKDGIIFSSLVPEVKIDSKP
  PSNEDVGLSMWSCDIGIGAKRSPSTSWFQKTCSTPSNPKYEMVIPDHPAKSHPDLHVSNDCSSSVAESSS
  PLRNFSCGFERTTRNEKLAAKTDEFNRTVFRTDRNCQAIQQNHSCSKSSEDLKPCDTSSTHTGSISQSND
  VSGIWKTNAHMPVPMENVPDNPTKKSTTGLVRQMQGHLSPRSYRNMLHEHDWRPSNLSGRPRSADPRSNY
  GVVEKLLKTYETATESALQNSKCFQDNWTKCNSDVSGGATLSQHLEMLQMEQQFQQKTAVWGGQEVKQGI
  DPKKITEESMSVNASHGKGFSRPARPANRRLPSRWASRSPSAPPALRRTTHNYTISLRSEALMV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014702
• ORF Size: 2082 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014702.5
• RefSeq Size: 8124 bp
• RefSeq ORF: 2085 bp
• Locus ID: 9729
• UniProt ID: Q6ZU52
• Cytogenetics: 6q22.33
• MW: 79.2 kDa