HLX1 (HLX) (NM_021958) Human Tagged ORF Clone

CAT#: RC203551

HLX (Myc-DDK-tagged)-Human H2.0-like homeobox (HLX)

Product Images

Circular map for RC203551

• 

  Western blot validation of overexpression lysate (Cat# LY402891) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203551 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HLX1
• Synonyms: HB24; HLX1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203551 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCGCAGCCGGGCTGGCTCCCTTCTACGCCTCCAACTTCAGCCTCTGGTCGGCCGCTTACTGCTCCT
  CGGCCGGCCCAGGCGGCTGCTCCTTCCCCTTGGACCCCGCCGCCGTCAAAAAGCCCTCCTTCTGCATCGC
  AGACATTCTGCACGCCGGCGTGGGGGATCTGGGGGCGGCCCCGGAGGGCCTGGCAGGGGCCTCGGCCGCC
  GCCCTCACCGCGCACTTGGGCTCGGTTCACCCGCACGCCTCTTTCCAAGCGGCGGCCAGATCCCCGCTTC
  GACCCACCCCAGTGGTGGCGCCCTCCGAAGTCCCGGCTGGCTTCCCGCAGCGGCTGTCTCCGCTCTCAGC
  CGCCTACCACCACCATCACCCGCAACAACAACAGCAGCAGCAACAGCCGCAGCAGCAACAGCCTCCGCCT
  CCGCCCCGGGCTGGCGCCCTGCAGCCCCCGGCCTCGGGGACGCGAGTGGTTCCGAACCCCCACCACAGTG
  GCTCTGCCCCGGCCCCCTCCAGCAAAGACCTCAAATTTGGAATTGACCGCATTTTATCTGCAGAATTTGA
  CCCAAAAGTCAAAGAAGGCAACACGCTGAGAGATCTCACTTCCCTGCTAACCGGTGGGCGGCCCGCCGGG
  GTGCACCTCTCAGGCCTGCAGCCCTCGGCCGGCCAGTTCTTCGCATCTCTAGATCCCATTAACGAGGCTT
  CTGCAATCCTGAGTCCCTTAAACTCGAACCCAAGAAATTCAGTTCAGCATCAGTTCCAAGACACGTTTCC
  AGGTCCCTATGCTGTGCTCACGAAGGACACCATGCCGCAGACGTACAAAAGGAAGCGTTCATGGTCGCGC
  GCTGTGTTCTCCAACCTGCAGAGGAAAGGCCTGGAGAAAAGGTTTGAGATTCAGAAGTACGTGACCAAGC
  CGGACCGAAAGCAGCTGGCGGCGATGCTGGGCCTCACGGACGCACAGGTGAAGGTGTGGTTCCAGAACCG
  GCGGATGAAGTGGCGGCACTCCAAGGAGGCCCAGGCCCAAAAGGACAAGGACAAGGAGGCTGGCGAGAAG
  CCATCAGGTGGAGCCCCGGCTGCGGATGGCGAGCAGGACGAGAGGAGCCCCAGCCGTTCTGAAGGCGAGG
  CTGAGAGCGAGAGCAGCGACTCCGAGTCCCTGGACATGGCCCCCAGCGACACGGAGCGGACTGAGGGGAG
  TGAGCGTTCTCTGCACCAAACAACAGTTATTAAGGCCCCGGTCACTGGCGCCCTCATTACCGCCAGCAGT
  GCTGGGAGTGGTGGGAGCAGCGGCGGCGGCGGCAATAGTTTCAGCTTCAGCAGCGCCAGCAGTCTTAGTA
  GCAGCAGCACCAGTGCGGGTTGCGCCAGCAGCCTTGGCGGCGGCGGCGCCTCGGAGCTTCTCCCTGCAAC
  ACAGCCCACAGCCAGCAGCGCTCCCAAAAGCCCCGAGCCAGCCCAAGGCGCGCTTGGCTGCTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203551 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFAAGLAPFYASNFSLWSAAYCSSAGPGGCSFPLDPAAVKKPSFCIADILHAGVGDLGAAPEGLAGASAA
  ALTAHLGSVHPHASFQAAARSPLRPTPVVAPSEVPAGFPQRLSPLSAAYHHHHPQQQQQQQQPQQQQPPP
  PPRAGALQPPASGTRVVPNPHHSGSAPAPSSKDLKFGIDRILSAEFDPKVKEGNTLRDLTSLLTGGRPAG
  VHLSGLQPSAGQFFASLDPINEASAILSPLNSNPRNSVQHQFQDTFPGPYAVLTKDTMPQTYKRKRSWSR
  AVFSNLQRKGLEKRFEIQKYVTKPDRKQLAAMLGLTDAQVKVWFQNRRMKWRHSKEAQAQKDKDKEAGEK
  PSGGAPAADGEQDERSPSRSEGEAESESSDSESLDMAPSDTERTEGSERSLHQTTVIKAPVTGALITASS
  AGSGGSSGGGGNSFSFSSASSLSSSSTSAGCASSLGGGGASELLPATQPTASSAPKSPEPAQGALGCL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021958
• ORF Size: 1464 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021958.4
• RefSeq Size: 2308 bp
• RefSeq ORF: 1467 bp
• Locus ID: 3142
• UniProt ID: Q14774
• Cytogenetics: 1q41
• Domains: homeobox
• Protein Families: Transcription Factors
• MW: 50.8 kDa
• Gene Summary: Transcription factor required for TBX21/T-bet-dependent maturation of Th1 cells as well as maintenance of Th1-specific gene expression. Involved in embryogenesis and hematopoiesis (By similarity).[UniProtKB/Swiss-Prot Function]