CPSF1 (NM_013291) Human Tagged ORF Clone

CAT#: RC203519

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CPSF1 (Myc-DDK-tagged)-Human cleavage and polyadenylation specific factor 1, 160kDa (CPSF1)



  "NM_013291" in other vectors (6)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 118.00

USD 880.00


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Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol CPSF1
Synonyms CPSF160; HSU37012; MYP27; P/cl.18
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203519 representing NM_013291
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTACGCCGTGTACAAACAGGCGCATCCGCCCACCGGTCTGGAGTTCTCCATGTACTGCAACTTCTTCA
ACAACAGCGAGCGCAACCTGGTAGTGGCCGGGACCTCGCAGCTCTACGTGTACCGCCTCAACCGCGACGC
CGAGGCTCTGACCAAGAATGACAGGAGCACAGAGGGGAAGGCCCACCGGGAGAAGCTCGAGCTTGCTGCC
TCCTTCTCCTTCTTTGGCAACGTCATGTCCATGGCCAGCGTGCAGCTGGCAGGAGCCAAGCGGGATGCCC
TGCTCCTAAGCTTCAAGGATGCCAAGCTGTCTGTGGTGGAGTACGACCCGGGCACCCATGACCTGAAGAC
CCTGTCACTGCACTACTTTGAGGAGCCTGAGCTTCGGGACGGGTTTGTGCAGAATGTACACACGCCGCGA
GTGCGGGTGGACCCCGACGGGCGCTGTGCAGCCATGCTTGTCTACGGCACGCGGCTGGTGGTCCTGCCCT
TCCGCAGGGAGAGCCTGGCTGAGGAGCACGAGGGGCTCGTGGGTGAGGGGCAGAGGTCCAGCTTCCTGCC
CAGCTACATCATCGACGTGCGGGCCCTAGACGAGAAGCTGCTCAACATCATCGACCTGCAGTTCCTGCAT
GGCTACTACGAGCCTACCCTCCTCATCCTGTTTGAGCCCAACCAGACCTGGCCTGGGCGCGTGGCCGTGC
GGCAGGACACGTGCTCCATTGTGGCCATCTCACTGAACATCACGCAGAAGGTGCACCCCGTCATCTGGTC
CCTCACCAGCCTGCCCTTTGACTGCACCCAGGCTCTGGCTGTGCCCAAGCCCATAGGTGGGGTGGTGGTG
TTTGCCGTCAACTCGCTGTTGTACCTGAACCAGAGCGTCCCCCCGTATGGCGTGGCTCTCAACAGCCTCA
CCACAGGAACCACGGCTTTCCCGCTTCGCACCCAGGAGGGTGTGCGGATCACCCTGGACTGCGCCCAGGC
CACCTTCATCTCCTACGACAAGATGGTCATCTCCCTCAAGGGCGGCGAGATCTACGTGCTGACCCTCATC
ACCGACGGCATGCGCAGTGTCCGAGCGTTCCACTTTGACAAGGCGGCCGCCAGCGTCCTCACCACCAGCA
TGGTCACCATGGAGCCCGGGTACCTGTTCCTGGGTTCTCGCCTGGGCAATTCCCTCCTCCTCAAGTACAC
GGAGAAGCTGCAGGAGCCCCCGGCCAGTGCTGTCCGTGAGGCTGCCGACAAGGAAGAGCCTCCCTCAAAG
AAGAAGCGAGTGGATGCGACGGCCGGCTGGTCAGCTGCGGGTAAGTCGGTGCCGCAGGATGAGGTGGACG
AGATTGAAGTGTACGGCAGCGAGGCCCAGTCGGGAACACAGCTGGCCACCTACTCCTTTGAGGTGTGTGA
CAGCATCCTGAACATTGGACCCTGTGCCAATGCCGCCGTGGGCGAGCCTGCCTTCCTCTCTGAAGAGTTT
CAGAACAGCCCCGAGCCGGACCTGGAGATTGTGGTTTGCTCCGGCCACGGGAAGAACGGGGCTTTGTCGG
TGCTGCAGAAGAGCATCCGGCCCCAGGTGGTGACAACCTTTGAGCTTCCCGGCTGCTATGACATGTGGAC
AGTCATCGCCCCGGTGCGTAAGGAGGAGGAGGACAATCCCAAGGGGGAGGGCACAGAGCAGGAACCCAGC
ACCACCCCTGAAGCAGACGACGACGGCCGCAGACACGGATTCCTGATTCTGAGCCGGGAAGACTCCACCA
TGATCCTGCAGACGGGGCAGGAGATCATGGAGCTGGACACCAGTGGCTTCGCCACTCAGGGCCCCACGGT
CTTTGCTGGGAACATCGGGGACAACCGCTACATTGTCCAAGTGTCACCACTGGGCATCCGCCTGCTGGAA
GGAGTGAATCAGCTGCACTTCATCCCCGTGGACCTGGGCGCCCCCATCGTGCAGTGCGCCGTGGCCGACC
CCTATGTGGTCATCATGAGTGCCGAGGGCCACGTCACCATGTTCCTGCTGAAGAGTGACTCCTACGGTGG
CCGCCACCACCGCCTGGCGCTGCACAAGCCCCCGCTGCACCATCAGTCCAAGGTGATTACGCTGTGCCTG
TACCGAGACCTCAGCGGCATGTTCACCACTGAGAGCCGCCTGGGTGGGGCCCGTGACGAGCTCGGGGGCC
GCAGTGGCCCGGAGGCCGAGGGCCTGGGCTCAGAGACTAGCCCCACAGTGGATGACGAGGAGGAGATGCT
GTATGGGGATTCGGGCTCCCTCTTCAGCCCCAGCAAGGAGGAGGCCCGAAGAAGCAGCCAGCCCCCTGCT
GACCGGGACCCTGCACCCTTCCGGGCAGAGCCTACCCACTGGTGCCTGCTGGTGCGGGAGAATGGCACCA
TGGAGATCTACCAGCTTCCCGACTGGCGGCTGGTGTTCCTGGTGAAGAACTTCCCTGTGGGGCAGCGGGT
CCTTGTGGACAGCTCCTTTGGACAGCCCACTACACAGGGCGAGGCCCGCAGGGAGGAGGCCACGCGCCAG
GGGGAGCTGCCCCTCGTCAAGGAGGTGCTGCTGGTGGCGCTGGGCAGCCGCCAGAGCAGGCCCTACCTGC
TGGTGCATGTGGACCAAGAGCTGCTTATCTACGAGGCCTTCCCCCACGACTCTCAGCTCGGCCAGGGCAA
TCTCAAAGTCCGCTTTAAGAAGGTCCCTCACAACATCAACTTCCGTGAGAAGAAGCCAAAGCCATCCAAG
AAGAAAGCAGAAGGTGGCGGCGCAGAGGAGGGGGCTGGGGCCCGGGGCCGCGTGGCGCGTTTCCGCTACT
TCGAGGATATTTATGGCTACTCAGGGGTCTTCATCTGCGGCCCCTCCCCTCACTGGCTCTTGGTGACCGG
CCGAGGGGCTCTGCGGCTACACCCCATGGCCATCGACGGCCCGGTCGACTCTTTCGCTCCATTCCACAAT
GTCAACTGTCCCCGCGGCTTCCTGTACTTCAACAGACAGGGCGAGCTGAGGATCAGTGTCCTGCCTGCCT
ACCTGTCCTATGATGCCCCATGGCCTGTCAGGAAGATCCCGCTGCGCTGCACGGCCCACTATGTGGCTTA
CCACGTGGAGTCTAAGGTGTATGCTGTGGCCACCAGCACCAACACGCCGTGTGCCCGCATCCCACGCATG
ACTGGCGAGGAGAAGGAGTTTGAGACCATCGAGAGAGATGAGCGGTACATCCACCCCCAGCAGGAGGCCT
TCTCCATCCAGCTCATCTCCCCGGTCAGCTGGGAGGCTATTCCCAATGCCAGGATCGAGCTGCAGGAGTG
GGAGCATGTGACCTGCATGAAGACAGTGTCTCTGCGCAGTGAGGAGACCGTGTCGGGCCTCAAAGGCTAC
GTGGCCGCCGGGACCTGCCTCATGCAGGGGGAGGAGGTCACGTGCCGAGGGCGGATCTTGATCATGGATG
TGATTGAGGTGGTGCCCGAGCCTGGCCAGCCCTTGACCAAGAACAAGTTCAAAGTCCTTTACGAGAAGGA
GCAGAAGGGGCCCGTGACCGCCCTGTGCCACTGCAATGGCCACCTGGTGTCGGCCATCGGCCAGAAGATT
TTCCTGTGGAGCCTGCGGGCCAGCGAGCTGACGGGCATGGCCTTCATCGACACGCAGCTCTACATACACC
AGATGATCAGCGTCAAGAACTTCATCCTGGCAGCCGACGTCATGAAGAGCATTTCGCTGCTGCGCTACCA
GGAGGAAAGCAAGACGCTGAGCCTGGTGTCGCGGGATGCCAAGCCCCTGGAGGTGTACAGCGTGGACTTC
ATGGTGGACAATGCCCAGCTGGGTTTTCTGGTGTCTGACCGCGACCGCAACCTCATGGTGTACATGTACC
TGCCCGAAGCCAAGGAGAGTTTCGGGGGCATGCGCCTGCTGCGTCGGGCAGACTTCCACGTGGGTGCCCA
CGTGAACACGTTCTGGAGGACCCCGTGCCGGGGGGCCACTGAAGGGCTCAGCAAAAAGTCGGTCGTGTGG
GAGAATAAGCACATCACGTGGTTTGCCACCCTGGACGGCGGCATCGGGCTGCTGCTGCCCATGCAGGAGA
AGACCTACCGGCGGCTGCTGATGCTGCAGAACGCGCTGACCACCATGCTGCCACACCACGCCGGCCTCAA
CCCCCGCGCCTTCCGGATGCTGCACGTGGACCGCCGCACCCTCCAGAATGCCGTGCGCAACGTGCTGGAT
GGGGAGCTGCTCAACCGCTACCTGTACCTGAGCACCATGGAGCGCAGCGAGCTAGCCAAGAAGATCGGCA
CCACACCAGACATAATCCTGGACGACTTGCTGGAGACGGACCGCGTCACCGCCCACTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203519 representing NM_013291
Red=Cloning site Green=Tags(s)

MYAVYKQAHPPTGLEFSMYCNFFNNSERNLVVAGTSQLYVYRLNRDAEALTKNDRSTEGKAHREKLELAA
SFSFFGNVMSMASVQLAGAKRDALLLSFKDAKLSVVEYDPGTHDLKTLSLHYFEEPELRDGFVQNVHTPR
VRVDPDGRCAAMLVYGTRLVVLPFRRESLAEEHEGLVGEGQRSSFLPSYIIDVRALDEKLLNIIDLQFLH
GYYEPTLLILFEPNQTWPGRVAVRQDTCSIVAISLNITQKVHPVIWSLTSLPFDCTQALAVPKPIGGVVV
FAVNSLLYLNQSVPPYGVALNSLTTGTTAFPLRTQEGVRITLDCAQATFISYDKMVISLKGGEIYVLTLI
TDGMRSVRAFHFDKAAASVLTTSMVTMEPGYLFLGSRLGNSLLLKYTEKLQEPPASAVREAADKEEPPSK
KKRVDATAGWSAAGKSVPQDEVDEIEVYGSEAQSGTQLATYSFEVCDSILNIGPCANAAVGEPAFLSEEF
QNSPEPDLEIVVCSGHGKNGALSVLQKSIRPQVVTTFELPGCYDMWTVIAPVRKEEEDNPKGEGTEQEPS
TTPEADDDGRRHGFLILSREDSTMILQTGQEIMELDTSGFATQGPTVFAGNIGDNRYIVQVSPLGIRLLE
GVNQLHFIPVDLGAPIVQCAVADPYVVIMSAEGHVTMFLLKSDSYGGRHHRLALHKPPLHHQSKVITLCL
YRDLSGMFTTESRLGGARDELGGRSGPEAEGLGSETSPTVDDEEEMLYGDSGSLFSPSKEEARRSSQPPA
DRDPAPFRAEPTHWCLLVRENGTMEIYQLPDWRLVFLVKNFPVGQRVLVDSSFGQPTTQGEARREEATRQ
GELPLVKEVLLVALGSRQSRPYLLVHVDQELLIYEAFPHDSQLGQGNLKVRFKKVPHNINFREKKPKPSK
KKAEGGGAEEGAGARGRVARFRYFEDIYGYSGVFICGPSPHWLLVTGRGALRLHPMAIDGPVDSFAPFHN
VNCPRGFLYFNRQGELRISVLPAYLSYDAPWPVRKIPLRCTAHYVAYHVESKVYAVATSTNTPCARIPRM
TGEEKEFETIERDERYIHPQQEAFSIQLISPVSWEAIPNARIELQEWEHVTCMKTVSLRSEETVSGLKGY
VAAGTCLMQGEEVTCRGRILIMDVIEVVPEPGQPLTKNKFKVLYEKEQKGPVTALCHCNGHLVSAIGQKI
FLWSLRASELTGMAFIDTQLYIHQMISVKNFILAADVMKSISLLRYQEESKTLSLVSRDAKPLEVYSVDF
MVDNAQLGFLVSDRDRNLMVYMYLPEAKESFGGMRLLRRADFHVGAHVNTFWRTPCRGATEGLSKKSVVW
ENKHITWFATLDGGIGLLLPMQEKTYRRLLMLQNALTTMLPHHAGLNPRAFRMLHVDRRTLQNAVRNVLD
GELLNRYLYLSTMERSELAKKIGTTPDIILDDLLETDRVTAHF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_013291
ORF Size 4329 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_013291.3
RefSeq Size 4494 bp
RefSeq ORF 4332 bp
Locus ID 29894
UniProt ID Q10570
Cytogenetics 8q24.3
Domains CPSF_A
MW 161.3 kDa
Gene Summary Cleavage and polyadenylation specificity factor (CPSF) is a multisubunit complex that plays a central role in 3-prime processing of pre-mRNAs. CPSF recognizes the AAUAAA signal in the pre-mRNA and interacts with other proteins to facilitate both RNA cleavage and poly(A) synthesis. CPSF1 is the largest subunit of the CPSF complex (Murthy and Manley, 1995 [PubMed 7590244]).[supplied by OMIM, Mar 2008]

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