CPSF1 (NM_013291) Human Tagged ORF Clone

CAT#: RC203519

CPSF1 (Myc-DDK-tagged)-Human cleavage and polyadenylation specific factor 1, 160kDa (CPSF1)

Product Images

Western blot validation of overexpression lysate (Cat# LY415687) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203519 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CPSF1
• Synonyms: CPSF160; HSU37012; MYP27; P/cl.18
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203519 representing NM_013291
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACGCCGTGTACAAACAGGCGCATCCGCCCACCGGTCTGGAGTTCTCCATGTACTGCAACTTCTTCA
  ACAACAGCGAGCGCAACCTGGTAGTGGCCGGGACCTCGCAGCTCTACGTGTACCGCCTCAACCGCGACGC
  CGAGGCTCTGACCAAGAATGACAGGAGCACAGAGGGGAAGGCCCACCGGGAGAAGCTCGAGCTTGCTGCC
  TCCTTCTCCTTCTTTGGCAACGTCATGTCCATGGCCAGCGTGCAGCTGGCAGGAGCCAAGCGGGATGCCC
  TGCTCCTAAGCTTCAAGGATGCCAAGCTGTCTGTGGTGGAGTACGACCCGGGCACCCATGACCTGAAGAC
  CCTGTCACTGCACTACTTTGAGGAGCCTGAGCTTCGGGACGGGTTTGTGCAGAATGTACACACGCCGCGA
  GTGCGGGTGGACCCCGACGGGCGCTGTGCAGCCATGCTTGTCTACGGCACGCGGCTGGTGGTCCTGCCCT
  TCCGCAGGGAGAGCCTGGCTGAGGAGCACGAGGGGCTCGTGGGTGAGGGGCAGAGGTCCAGCTTCCTGCC
  CAGCTACATCATCGACGTGCGGGCCCTAGACGAGAAGCTGCTCAACATCATCGACCTGCAGTTCCTGCAT
  GGCTACTACGAGCCTACCCTCCTCATCCTGTTTGAGCCCAACCAGACCTGGCCTGGGCGCGTGGCCGTGC
  GGCAGGACACGTGCTCCATTGTGGCCATCTCACTGAACATCACGCAGAAGGTGCACCCCGTCATCTGGTC
  CCTCACCAGCCTGCCCTTTGACTGCACCCAGGCTCTGGCTGTGCCCAAGCCCATAGGTGGGGTGGTGGTG
  TTTGCCGTCAACTCGCTGTTGTACCTGAACCAGAGCGTCCCCCCGTATGGCGTGGCTCTCAACAGCCTCA
  CCACAGGAACCACGGCTTTCCCGCTTCGCACCCAGGAGGGTGTGCGGATCACCCTGGACTGCGCCCAGGC
  CACCTTCATCTCCTACGACAAGATGGTCATCTCCCTCAAGGGCGGCGAGATCTACGTGCTGACCCTCATC
  ACCGACGGCATGCGCAGTGTCCGAGCGTTCCACTTTGACAAGGCGGCCGCCAGCGTCCTCACCACCAGCA
  TGGTCACCATGGAGCCCGGGTACCTGTTCCTGGGTTCTCGCCTGGGCAATTCCCTCCTCCTCAAGTACAC
  GGAGAAGCTGCAGGAGCCCCCGGCCAGTGCTGTCCGTGAGGCTGCCGACAAGGAAGAGCCTCCCTCAAAG
  AAGAAGCGAGTGGATGCGACGGCCGGCTGGTCAGCTGCGGGTAAGTCGGTGCCGCAGGATGAGGTGGACG
  AGATTGAAGTGTACGGCAGCGAGGCCCAGTCGGGAACACAGCTGGCCACCTACTCCTTTGAGGTGTGTGA
  CAGCATCCTGAACATTGGACCCTGTGCCAATGCCGCCGTGGGCGAGCCTGCCTTCCTCTCTGAAGAGTTT
  CAGAACAGCCCCGAGCCGGACCTGGAGATTGTGGTTTGCTCCGGCCACGGGAAGAACGGGGCTTTGTCGG
  TGCTGCAGAAGAGCATCCGGCCCCAGGTGGTGACAACCTTTGAGCTTCCCGGCTGCTATGACATGTGGAC
  AGTCATCGCCCCGGTGCGTAAGGAGGAGGAGGACAATCCCAAGGGGGAGGGCACAGAGCAGGAACCCAGC
  ACCACCCCTGAAGCAGACGACGACGGCCGCAGACACGGATTCCTGATTCTGAGCCGGGAAGACTCCACCA
  TGATCCTGCAGACGGGGCAGGAGATCATGGAGCTGGACACCAGTGGCTTCGCCACTCAGGGCCCCACGGT
  CTTTGCTGGGAACATCGGGGACAACCGCTACATTGTCCAAGTGTCACCACTGGGCATCCGCCTGCTGGAA
  GGAGTGAATCAGCTGCACTTCATCCCCGTGGACCTGGGCGCCCCCATCGTGCAGTGCGCCGTGGCCGACC
  CCTATGTGGTCATCATGAGTGCCGAGGGCCACGTCACCATGTTCCTGCTGAAGAGTGACTCCTACGGTGG
  CCGCCACCACCGCCTGGCGCTGCACAAGCCCCCGCTGCACCATCAGTCCAAGGTGATTACGCTGTGCCTG
  TACCGAGACCTCAGCGGCATGTTCACCACTGAGAGCCGCCTGGGTGGGGCCCGTGACGAGCTCGGGGGCC
  GCAGTGGCCCGGAGGCCGAGGGCCTGGGCTCAGAGACTAGCCCCACAGTGGATGACGAGGAGGAGATGCT
  GTATGGGGATTCGGGCTCCCTCTTCAGCCCCAGCAAGGAGGAGGCCCGAAGAAGCAGCCAGCCCCCTGCT
  GACCGGGACCCTGCACCCTTCCGGGCAGAGCCTACCCACTGGTGCCTGCTGGTGCGGGAGAATGGCACCA
  TGGAGATCTACCAGCTTCCCGACTGGCGGCTGGTGTTCCTGGTGAAGAACTTCCCTGTGGGGCAGCGGGT
  CCTTGTGGACAGCTCCTTTGGACAGCCCACTACACAGGGCGAGGCCCGCAGGGAGGAGGCCACGCGCCAG
  GGGGAGCTGCCCCTCGTCAAGGAGGTGCTGCTGGTGGCGCTGGGCAGCCGCCAGAGCAGGCCCTACCTGC
  TGGTGCATGTGGACCAAGAGCTGCTTATCTACGAGGCCTTCCCCCACGACTCTCAGCTCGGCCAGGGCAA
  TCTCAAAGTCCGCTTTAAGAAGGTCCCTCACAACATCAACTTCCGTGAGAAGAAGCCAAAGCCATCCAAG
  AAGAAAGCAGAAGGTGGCGGCGCAGAGGAGGGGGCTGGGGCCCGGGGCCGCGTGGCGCGTTTCCGCTACT
  TCGAGGATATTTATGGCTACTCAGGGGTCTTCATCTGCGGCCCCTCCCCTCACTGGCTCTTGGTGACCGG
  CCGAGGGGCTCTGCGGCTACACCCCATGGCCATCGACGGCCCGGTCGACTCTTTCGCTCCATTCCACAAT
  GTCAACTGTCCCCGCGGCTTCCTGTACTTCAACAGACAGGGCGAGCTGAGGATCAGTGTCCTGCCTGCCT
  ACCTGTCCTATGATGCCCCATGGCCTGTCAGGAAGATCCCGCTGCGCTGCACGGCCCACTATGTGGCTTA
  CCACGTGGAGTCTAAGGTGTATGCTGTGGCCACCAGCACCAACACGCCGTGTGCCCGCATCCCACGCATG
  ACTGGCGAGGAGAAGGAGTTTGAGACCATCGAGAGAGATGAGCGGTACATCCACCCCCAGCAGGAGGCCT
  TCTCCATCCAGCTCATCTCCCCGGTCAGCTGGGAGGCTATTCCCAATGCCAGGATCGAGCTGCAGGAGTG
  GGAGCATGTGACCTGCATGAAGACAGTGTCTCTGCGCAGTGAGGAGACCGTGTCGGGCCTCAAAGGCTAC
  GTGGCCGCCGGGACCTGCCTCATGCAGGGGGAGGAGGTCACGTGCCGAGGGCGGATCTTGATCATGGATG
  TGATTGAGGTGGTGCCCGAGCCTGGCCAGCCCTTGACCAAGAACAAGTTCAAAGTCCTTTACGAGAAGGA
  GCAGAAGGGGCCCGTGACCGCCCTGTGCCACTGCAATGGCCACCTGGTGTCGGCCATCGGCCAGAAGATT
  TTCCTGTGGAGCCTGCGGGCCAGCGAGCTGACGGGCATGGCCTTCATCGACACGCAGCTCTACATACACC
  AGATGATCAGCGTCAAGAACTTCATCCTGGCAGCCGACGTCATGAAGAGCATTTCGCTGCTGCGCTACCA
  GGAGGAAAGCAAGACGCTGAGCCTGGTGTCGCGGGATGCCAAGCCCCTGGAGGTGTACAGCGTGGACTTC
  ATGGTGGACAATGCCCAGCTGGGTTTTCTGGTGTCTGACCGCGACCGCAACCTCATGGTGTACATGTACC
  TGCCCGAAGCCAAGGAGAGTTTCGGGGGCATGCGCCTGCTGCGTCGGGCAGACTTCCACGTGGGTGCCCA
  CGTGAACACGTTCTGGAGGACCCCGTGCCGGGGGGCCACTGAAGGGCTCAGCAAAAAGTCGGTCGTGTGG
  GAGAATAAGCACATCACGTGGTTTGCCACCCTGGACGGCGGCATCGGGCTGCTGCTGCCCATGCAGGAGA
  AGACCTACCGGCGGCTGCTGATGCTGCAGAACGCGCTGACCACCATGCTGCCACACCACGCCGGCCTCAA
  CCCCCGCGCCTTCCGGATGCTGCACGTGGACCGCCGCACCCTCCAGAATGCCGTGCGCAACGTGCTGGAT
  GGGGAGCTGCTCAACCGCTACCTGTACCTGAGCACCATGGAGCGCAGCGAGCTAGCCAAGAAGATCGGCA
  CCACACCAGACATAATCCTGGACGACTTGCTGGAGACGGACCGCGTCACCGCCCACTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203519 representing NM_013291
  Red=Cloning site Green=Tags(s)
  
  MYAVYKQAHPPTGLEFSMYCNFFNNSERNLVVAGTSQLYVYRLNRDAEALTKNDRSTEGKAHREKLELAA
  SFSFFGNVMSMASVQLAGAKRDALLLSFKDAKLSVVEYDPGTHDLKTLSLHYFEEPELRDGFVQNVHTPR
  VRVDPDGRCAAMLVYGTRLVVLPFRRESLAEEHEGLVGEGQRSSFLPSYIIDVRALDEKLLNIIDLQFLH
  GYYEPTLLILFEPNQTWPGRVAVRQDTCSIVAISLNITQKVHPVIWSLTSLPFDCTQALAVPKPIGGVVV
  FAVNSLLYLNQSVPPYGVALNSLTTGTTAFPLRTQEGVRITLDCAQATFISYDKMVISLKGGEIYVLTLI
  TDGMRSVRAFHFDKAAASVLTTSMVTMEPGYLFLGSRLGNSLLLKYTEKLQEPPASAVREAADKEEPPSK
  KKRVDATAGWSAAGKSVPQDEVDEIEVYGSEAQSGTQLATYSFEVCDSILNIGPCANAAVGEPAFLSEEF
  QNSPEPDLEIVVCSGHGKNGALSVLQKSIRPQVVTTFELPGCYDMWTVIAPVRKEEEDNPKGEGTEQEPS
  TTPEADDDGRRHGFLILSREDSTMILQTGQEIMELDTSGFATQGPTVFAGNIGDNRYIVQVSPLGIRLLE
  GVNQLHFIPVDLGAPIVQCAVADPYVVIMSAEGHVTMFLLKSDSYGGRHHRLALHKPPLHHQSKVITLCL
  YRDLSGMFTTESRLGGARDELGGRSGPEAEGLGSETSPTVDDEEEMLYGDSGSLFSPSKEEARRSSQPPA
  DRDPAPFRAEPTHWCLLVRENGTMEIYQLPDWRLVFLVKNFPVGQRVLVDSSFGQPTTQGEARREEATRQ
  GELPLVKEVLLVALGSRQSRPYLLVHVDQELLIYEAFPHDSQLGQGNLKVRFKKVPHNINFREKKPKPSK
  KKAEGGGAEEGAGARGRVARFRYFEDIYGYSGVFICGPSPHWLLVTGRGALRLHPMAIDGPVDSFAPFHN
  VNCPRGFLYFNRQGELRISVLPAYLSYDAPWPVRKIPLRCTAHYVAYHVESKVYAVATSTNTPCARIPRM
  TGEEKEFETIERDERYIHPQQEAFSIQLISPVSWEAIPNARIELQEWEHVTCMKTVSLRSEETVSGLKGY
  VAAGTCLMQGEEVTCRGRILIMDVIEVVPEPGQPLTKNKFKVLYEKEQKGPVTALCHCNGHLVSAIGQKI
  FLWSLRASELTGMAFIDTQLYIHQMISVKNFILAADVMKSISLLRYQEESKTLSLVSRDAKPLEVYSVDF
  MVDNAQLGFLVSDRDRNLMVYMYLPEAKESFGGMRLLRRADFHVGAHVNTFWRTPCRGATEGLSKKSVVW
  ENKHITWFATLDGGIGLLLPMQEKTYRRLLMLQNALTTMLPHHAGLNPRAFRMLHVDRRTLQNAVRNVLD
  GELLNRYLYLSTMERSELAKKIGTTPDIILDDLLETDRVTAHF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013291
• ORF Size: 4329 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013291.3
• RefSeq Size: 4494 bp
• RefSeq ORF: 4332 bp
• Locus ID: 29894
• UniProt ID: Q10570
• Cytogenetics: 8q24.3
• Domains: CPSF_A
• MW: 161.3 kDa
• Gene Summary: Cleavage and polyadenylation specificity factor (CPSF) is a multisubunit complex that plays a central role in 3-prime processing of pre-mRNAs. CPSF recognizes the AAUAAA signal in the pre-mRNA and interacts with other proteins to facilitate both RNA cleavage and poly(A) synthesis. CPSF1 is the largest subunit of the CPSF complex (Murthy and Manley, 1995 [PubMed 7590244]).[supplied by OMIM, Mar 2008]