SCRN2 (NM_138355) Human Tagged ORF Clone

CAT#: RC203501

SCRN2 (Myc-DDK-tagged)-Human secernin 2 (SCRN2), transcript variant 1

Product Images

Circular map for RC203501

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  Western blot validation of overexpression lysate (Cat# LY408627) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203501 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SCRN2 protein (Cat# TP303501). The protein was produced from HEK293T cells transfected with SCRN2 cDNA clone (Cat# RC203501) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SCRN2
• Synonyms: Ses2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203501 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCGTCGAGCCCTGACTCCCCATGTTCCTGCGACTGCTTTGTCTCCGTGCCCCCGGCCTCAGCCA
  TCCCGGCTGTGATCTTTGCCAAGAACTCGGACCGACCCCGGGACGAGGTGCAGGAGGTGGTGTTTGTCCC
  CGCAGGCACTCACACTCCTGGGAGCCGGCTCCAGTGCACCTACATTGAAGTGGAACAGGTGTCGAAGACG
  CACGCTGTGATTCTGAGCCGTCCTTCTTGGCTATGGGGGGCTGAGATGGGCGCCAACGAGCATGGTGTCT
  GCATTGGCAACGAGGCTGTGTGGACGAAGGAGCCAGTTGGGGAGGGGGAAGCCCTGCTGGGCATGGACCT
  ACTCAGGCTGGCTTTGGAACGGAGCAGCTCTGCCCAGGAGGCCTTGCATGTGATCACAGGGTTACTGGAG
  CACTATGGGCAGGGGGGCAACTGCCTGGAGGATGCTGCGCCATTCTCCTACCATAGCACCTTCCTGCTGG
  CTGACCGCACTGAGGCGTGGGTGCTGGAGACAGCTGGGAGGCTCTGGGCTGCACAGAGGATCCAGGAGGG
  GGCCCGCAACATCTCCAACCAGCTGAGCATTGGCACGGACATCTCGGCCCAACACCCGGAGCTGCGGACT
  CATGCCCAGGCCAAGGGCTGGTGGGATGGGCAGGGTGCCTTTGACTTTGCTCAGATCTTCTCCCTGACCC
  AGCAGCCTGTGCGCATGGAGGCTGCCAAGGCCCGCTTCCAGGCAGGGCGGGAGCTGCTGCGGCAACGGCA
  AGGGGGCATCACGGCAGAGGTGATGATGGGCATCCTCAGAGACAAGGAGAGTGGTATCTGTATGGACTCG
  GGAGGCTTTCGCACCACGGCCAGCATGGTGTCTGTCCTGCCCCAGGATCCCACGCAGCCCTGCGTGCACT
  TTCTTACCGCCACGCCAGACCCATCCAGGTCTGTGTTCAAACCTTTCATCTTCGGGGTGGGGGTGGCCCA
  GGCCCCCCAGGTGCTGTCCCCCACTTTTGGAGCACAAGACCCTGTTCGGACCCTGCCCCGATTCCAGACT
  CAGGTAGATCGTCGGCATACCCTCTACCGTGGACACCAGGCAGCCCTGGGGCTGATGGAGAGAGATCAGG
  ATCGGGGGCAGCAGCTCCAGCAGAAACAGCAGGATCTGGAGCAGGAAGGCCTCGAGGCCACACAGGGGCT
  GCTGGCCGGCGAGTGGGCCCCACCCCTCTGGGAGCTGGGCAGCCTCTTCCAGGCCTTCGTGAAGAGGGAG
  AGCCAGGCTTATGCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203501 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASSSPDSPCSCDCFVSVPPASAIPAVIFAKNSDRPRDEVQEVVFVPAGTHTPGSRLQCTYIEVEQVSKT
  HAVILSRPSWLWGAEMGANEHGVCIGNEAVWTKEPVGEGEALLGMDLLRLALERSSSAQEALHVITGLLE
  HYGQGGNCLEDAAPFSYHSTFLLADRTEAWVLETAGRLWAAQRIQEGARNISNQLSIGTDISAQHPELRT
  HAQAKGWWDGQGAFDFAQIFSLTQQPVRMEAAKARFQAGRELLRQRQGGITAEVMMGILRDKESGICMDS
  GGFRTTASMVSVLPQDPTQPCVHFLTATPDPSRSVFKPFIFGVGVAQAPQVLSPTFGAQDPVRTLPRFQT
  QVDRRHTLYRGHQAALGLMERDQDRGQQLQQKQQDLEQEGLEATQGLLAGEWAPPLWELGSLFQAFVKRE
  SQAYA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138355
• ORF Size: 1275 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138355.4
• RefSeq Size: 1582 bp
• RefSeq ORF: 1278 bp
• Locus ID: 90507
• UniProt ID: Q96FV2
• Cytogenetics: 17q21.32
• MW: 46.6 kDa