ZBTB5 (NM_014872) Human Tagged ORF Clone

CAT#: RC203443

ZBTB5 (Myc-DDK-tagged)-Human zinc finger and BTB domain containing 5 (ZBTB5)

Product Images

Circular map for RC203443

• 

  Western blot validation of overexpression lysate (Cat# LY414902) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203443 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZBTB5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203443 representing NM_014872
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTTCCTGGTCACTTTGAACAAATCTTCCAGCAGCTGAACTACCAGAGACTTCATGGCCAGCTCT
  GTGATTGTGTCATTGTAGTGGGGAATAGACACTTTAAAGCCCACCGCTCCGTGCTGGCAGCATGCAGCAC
  GCATTTCCGAGCCCTGTTCTCAGTGGCAGAAGGAGATCAGACCATGAACATGATCCAGCTGGATAGCGAG
  GTGGTGACAGCAGAGGCCTTTGCTGCACTGATTGACATGATGTATACCTCCACCCTCATGCTGGGGGAGA
  GCAATGTAATGGATGTCTTATTGGCAGCCTCTCACCTGCATTTGAACTCTGTTGTTAAGGCATGTAAACA
  TTACTTAACGACAAGGACGCTGCCCATGTCTCCCCCCAGTGAGCGCGTTCAGGAGCAGAGCGCCCGCATG
  CAGCGCTCCTTTATGCTACAGCAGCTGGGACTAAGCATCGTGAGCTCAGCCCTCAATTCCAGCCAGAATG
  GCGAGGAGCAGCCAGCCCCCATGAGCTCTTCCATGCGCAGTAACCTGGATCAGCGCACGCCCTTCCCCAT
  GAGACGCCTTCATAAGCGCAAGCAGTCTGCAGAGGAGCGGGCCAGGCAGCGCCTCCGACCCTCCATAGAT
  GAGTCTGCCATTTCAGATGTTACACCGGAGAATGGGCCTTCAGGGGTTCATTCTCGGGAGGAGTTCTTTT
  CACCAGATTCTCTGAAAATTGTGGATAATCCTAAAGCTGACGGAATGACTGATAACCAGGAAGATAGTGC
  GATCATGTTTGATCAGTCTTTTGGCACTCAAGAAGATGCCCAGGTGCCCAGCCAGTCTGATAACAGTGCT
  GGCAACATGGCACAGTTGTCCATGGCCTCTCGTGCAACTCAGGTTGAGACTAGTTTTGATCAGGAAGCTG
  CACCTGAGAAAAGTAGTTTTCAGTGTGAAAACCCTGAGGTTGGCCTTGGTGAGAAGGAGCACATGAGAGT
  GGTGGTTAAATCTGAGCCCCTGAGCTCACCTGAGCCTCAGGATGAAGTGAGCGATGTGACCTCACAAGCA
  GAAGGCAGCGAGTCTGTGGAAGTGGAAGGAGTTGTGGTCAGTGCCGAGAAGATAGACCTCAGCCCTGAAA
  GCAGTGATCGGAGTTTTTCAGATCCCCAGTCTAGCACAGACAGGGTAGGTGATATCCATATTTTGGAAGT
  CACAAATAACCTAGAGCATAAGTCCACTTTTAGTATTTCGAATTTTCTTAACAAGAGCAGAGGAAATAAC
  TTTACTGCAAATCAGAACAATGATGATAATATTCCAAACACCACTAGTGACTGCAGGCTGGAGAGTGAGG
  CCCCCTATTTGTTGAGTCCAGAGGCTGGGCCTGCAGGTGGGCCCTCCTCTGCCCCTGGCTCCCATGTAGA
  GAACCCATTTAGTGAACCTGCAGACTCCCACTTCGTCAGGCCTATGCAGGAGGTGATGGGCCTGCCGTGT
  GTGCAGACTTCAGGCTACCAAGGAGGAGAACAGTTTGGGATGGACTTTTCCAGGTCTGGTTTGGGCCTCC
  ACTCCTCCTTCTCCAGGGTAATGATAGGTTCCCCAAGGGGAGGAGCCAGTAACTTTCCTTACTACCGCCG
  CATAGCTCCCAAAATGCCAGTTGTAACTTCCGTCAGGAGCTCACAGATCCCAGAAAACTCTACCAGTTCT
  CAGCTAATGATGAATGGAGCTACGTCCTCATTTGAAAATGGCCATCCTTCCCAGCCTGGCCCTCCACAGT
  TGACCAGGGCATCTGCAGATGTTCTGTCAAAGTGCAAGAAGGCCTTATCAGAGCACAATGTTTTGGTTGT
  AGAGGGAGCTCGCAAGTATGCCTGCAAAATCTGCTGCAAAACTTTTCTGACTTTGACAGATTGCAAGAAG
  CACATCCGTGTTCACACAGGTGAAAAGCCTTACGCCTGCCTGAAGTGTGGCAAGAGGTTTAGTCAGTCCA
  GCCACCTGTATAAGCACTCAAAGACTACCTGCCTGCGCTGGCAGAGCAGCAATCTTCCCAGCACTTTGCT
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203443 representing NM_014872
  Red=Cloning site Green=Tags(s)
  
  MDFPGHFEQIFQQLNYQRLHGQLCDCVIVVGNRHFKAHRSVLAACSTHFRALFSVAEGDQTMNMIQLDSE
  VVTAEAFAALIDMMYTSTLMLGESNVMDVLLAASHLHLNSVVKACKHYLTTRTLPMSPPSERVQEQSARM
  QRSFMLQQLGLSIVSSALNSSQNGEEQPAPMSSSMRSNLDQRTPFPMRRLHKRKQSAEERARQRLRPSID
  ESAISDVTPENGPSGVHSREEFFSPDSLKIVDNPKADGMTDNQEDSAIMFDQSFGTQEDAQVPSQSDNSA
  GNMAQLSMASRATQVETSFDQEAAPEKSSFQCENPEVGLGEKEHMRVVVKSEPLSSPEPQDEVSDVTSQA
  EGSESVEVEGVVVSAEKIDLSPESSDRSFSDPQSSTDRVGDIHILEVTNNLEHKSTFSISNFLNKSRGNN
  FTANQNNDDNIPNTTSDCRLESEAPYLLSPEAGPAGGPSSAPGSHVENPFSEPADSHFVRPMQEVMGLPC
  VQTSGYQGGEQFGMDFSRSGLGLHSSFSRVMIGSPRGGASNFPYYRRIAPKMPVVTSVRSSQIPENSTSS
  QLMMNGATSSFENGHPSQPGPPQLTRASADVLSKCKKALSEHNVLVVEGARKYACKICCKTFLTLTDCKK
  HIRVHTGEKPYACLKCGKRFSQSSHLYKHSKTTCLRWQSSNLPSTLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014872
• ORF Size: 2031 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014872.3
• RefSeq Size: 4631 bp
• RefSeq ORF: 2034 bp
• Locus ID: 9925
• UniProt ID: O15062
• Cytogenetics: 9p13.2
• Domains: BTB, zf-C2H2
• Protein Families: Transcription Factors
• MW: 74.1 kDa
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]