CBX8 (NM_020649) Human Tagged ORF Clone

CAT#: RC203417

CBX8 (Myc-DDK-tagged)-Human chromobox homolog 8 (CBX8)

Product Images

Circular map for RC203417

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CBX8 (Cat# RC203417, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CBX8(Cat# TA800617). Positive lysates LY412158 (100ug) and LC412158 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY412158) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203417 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CBX8
• Synonyms: PC3; RC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203417 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTTTCAGCGGTGGGGGAGCGGGTGTTCGCGGCCGAAGCCCTCCTGAAGCGGCGCATACGGAAAG
  GACGCATGGAATACCTCGTGAAATGGAAGGGATGGTCGCAGAAGTACAGCACATGGGAACCGGAGGAAAA
  CATCCTGGATGCTCGCTTGCTCGCAGCCTTTGAGGAAAGGGAAAGAGAGATGGAGCTCTATGGCCCCAAA
  AAGCGTGGACCCAAGCCCAAAACCTTCCTCCTCAAAGCGCAGGCCAAGGCAAAGGCCAAAACTTACGAGT
  TTCGAAGTGACTCAGCCAGGGGCATCCGGATCCCCTACCCTGGCCGCTCGCCCCAGGACCTGGCCTCCAC
  TTCCCGGGCCCGGGAGGGCCTTCGAAACATGGGTTTGTCCCCGCCAGCGAGCAGCACCAGCACCAGCAGC
  ACCTGCCGCGCAGAGGCCCCTCGGGACCGGGACCGAGACCGGGATAGGGACCGGGAGCGGGATCGAGAAA
  GGGAGAGGGAGCGAGAGAGGGAGCGGGAACGTGAGAGGGAACGAGAGCGGGGTACCAGCAGAGTGGATGA
  CAAGCCCAGCTCACCGGGGGACAGCTCGAAGAAGCGAGGCCCCAAGCCCCGGAAGGAGCTCCCGGACCCC
  TCACAGAGGCCCTTAGGCGAACCCAGCGCCGGCCTCGGAGAGTACCTCAAGGGCAGGAAGCTGGACGACA
  CCCCTTCCGGGGCAGGAAAGTTTCCAGCCGGCCACAGTGTGATCCAGCTGGCCCGAAGACAGGACTCGGA
  CCTGGTGCAGTGTGGTGTGACCAGCCCTAGCTCAGCTGAGGCCACGGGCAAACTGGCTGTGGACACCTTC
  CCGGCCAGGGTGATAAAGCACAGGGCTGCCTTCCTGGAGGCCAAAGGCCAGGGTGCCCTAGATCCCAATG
  GCACCCGGGTCCGACATGGCTCAGGCCCCCCCAGCTCTGGGGGGGGCCTGTACCGGGACATGGGGGCCCA
  GGGGGGAAGGCCCTCCCTCATCGCCAGGATCCCTGTGGCCAGAATCCTGGGGGACCCGGAGGAAGAGTCC
  TGGAGCCCCTCCCTGACTAACCTGGAGAAGGTGGTGGTCACGGACGTGACCTCAAACTTTTTGACCGTCA
  CCATTAAGGAAAGTAACACGGACCAAGGCTTTTTTAAAGAGAAAAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203417 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MELSAVGERVFAAEALLKRRIRKGRMEYLVKWKGWSQKYSTWEPEENILDARLLAAFEEREREMELYGPK
  KRGPKPKTFLLKAQAKAKAKTYEFRSDSARGIRIPYPGRSPQDLASTSRAREGLRNMGLSPPASSTSTSS
  TCRAEAPRDRDRDRDRDRERDRERERERERERERERERERGTSRVDDKPSSPGDSSKKRGPKPRKELPDP
  SQRPLGEPSAGLGEYLKGRKLDDTPSGAGKFPAGHSVIQLARRQDSDLVQCGVTSPSSAEATGKLAVDTF
  PARVIKHRAAFLEAKGQGALDPNGTRVRHGSGPPSSGGGLYRDMGAQGGRPSLIARIPVARILGDPEEES
  WSPSLTNLEKVVVTDVTSNFLTVTIKESNTDQGFFKEKR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020649
• ORF Size: 1167 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020649.3
• RefSeq Size: 1561 bp
• RefSeq ORF: 1170 bp
• Locus ID: 57332
• UniProt ID: Q9HC52
• Cytogenetics: 17q25.3
• Domains: CHROMO
• Protein Families: Transcription Factors
• MW: 43.4 kDa
• Gene Summary: Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.[UniProtKB/Swiss-Prot Function]