Cullin 2 (CUL2) (NM_003591) Human Tagged ORF Clone

CAT#: RC203304

CUL2 (Myc-DDK-tagged)-Human cullin 2 (CUL2), transcript variant 3

Product Images

Circular map for RC203304

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  Western blot validation of overexpression lysate (Cat# LY418560) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203304 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CUL2 protein (Cat# TP303304). The protein was produced from HEK293T cells transfected with CUL2 cDNA clone (Cat# RC203304) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cullin 2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203304 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTTGAAACCAAGAGTAGTAGATTTTGATGAAACATGGAACAAACTTTTGACGACAATAAAAGCCG
  TGGTCATGTTGGAATACGTCGAAAGAGCAACATGGAATGACCGTTTCTCAGATATCTATGCTTTATGTGT
  GGCCTATCCTGAACCCCTTGGAGAAAGACTTTATACAGAAACTAAGATTTTTTTGGAAAATCATGTTCGG
  CATTTGCATAAGAGAGTTTTGGAGTCAGAAGAACAAGTACTTGTTATGTATCATAGGTACTGGGAAGAAT
  ACAGCAAGGGTGCAGACTATATGGACTGCTTATATAGGTATCTCAACACCCAGTTTATTAAAAAGAATAA
  ATTAACAGAAGCGGACCTTCAGTATGGCTATGGTGGTGTAGATATGAATGAACCACTTATGGAAATAGGA
  GAGCTAGCATTGGATATGTGGAGGAAATTGATGGTTGAACCACTTCAGGCCATCCTTATCCGAATGCTGC
  TCCGAGAAATCAAAAATGATCGTGGTGGAGAAGACCCAAACCAGAAAGTAATCCATGGGGTTATTAACTC
  CTTTGTTCATGTTGAACAGTATAAGAAAAAATTCCCCTTAAAGTTTTATCAGGAAATTTTTGAGTCTCCC
  TTTCTGACTGAAACAGGAGAGTATTACAAACAAGAAGCTTCAAATTTATTACAAGAATCAAACTGCTCAC
  AGTATATGGAAAAGGTTCTAGGTAGATTAAAAGATGAAGAAATTCGATGTCGAAAATACCTACATCCAAG
  TTCATATACTAAGGTGATTCATGAATGTCAACAACGAATGGTAGCAGACCACTTACAGTTTTTACATGCA
  GAATGTCATAATATAATTCGACAAGAGAAAAAAAATGACATGGCAAATATGTACGTCTTACTCCGTGCTG
  TGTCCACTGGTTTACCTCATATGATTCAGGAGCTGCAAAACCACATCCATGATGAGGGCCTTCGAGCAAC
  CAGCAACCTTACTCAGGAAAACATGCCAACACTATTTGTGGAGTCAGTTTTGGAAGTGCATGGTAAATTT
  GTTCAGCTTATCAACACTGTTTTGAATGGTGATCAGCATTTTATGAGTGCGTTGGATAAGGCCCTTACGT
  CAGTTGTAAATTACAGAGAACCTAAGTCTGTTTGCAAAGCACCTGAACTGCTTGCTAAGTACTGTGACAA
  CTTACTGAAGAAGTCAGCGAAAGGGATGACAGAGAATGAAGTGGAAGACAGGCTCACGAGCTTCATCACA
  GTGTTCAAATACATTGATGACAAGGACGTCTTTCAAAAGTTCTACGCAAGAATGCTGGCAAAACGTTTAA
  TTCATGGGTTATCCATGTCTATGGACTCTGAAGAAGCCATGATCAACAAATTAAAGCAAGCCTGTGGTTA
  TGAGTTTACCAGCAAGCTACATCGGATGTATACAGATATGAGTGTCAGCGCTGATCTCAACAATAAGTTC
  AACAATTTTATCAAAAACCAAGACACAGTAATAGATTTGGGAATTAGTTTTCAAATATATGTTCTACAGG
  CTGGTGCGTGGCCTCTTACTCAGGCTCCTTCATCTACGTTTGCAATTCCCCAGGAATTAGAAAAAAGTGT
  ACAGATGTTTGAATTATTTTATAGCCAACATTTCAGTGGAAGGAAACTTACATGGTTACATTATCTGTGT
  ACAGGTGAAGTTAAAATGAACTATTTGGGCAAACCATATGTAGCCATGGTTACAACATACCAAATGGCAG
  TTCTTCTTGCCTTTAACAACAGTGAAACTGTCAGTTATAAAGAGCTTCAGGACAGCACTCAGATGAATGA
  AAAGGAACTGACAAAAACAATCAAATCATTACTTGATGTGAAAATGATTAACCATGATTCAGAAAAGGAA
  GATATTGATGCAGAATCTTCGTTTTCATTAAATATGAACTTTAGCAGTAAAAGAACAAAATTTAAAATTA
  CTACATCAATGCAGAAAGACACACCACAAGAAATGGAGCAGACTAGAAGTGCAGTTGATGAGGACCGGAA
  AATGTATCTCCAAGCTGCTATAGTTCGTATCATGAAAGCACGAAAAGTGCTTCGGCACAATGCCCTTATT
  CAAGAGGTGATTAGCCAGTCAAGAGCTAGGTTTAATCCCAGTATCAGCATGATTAAGAAGTGTATTGAAG
  TTCTGATAGACAAACAATACATAGAACGCAGCCAGGCGTCGGCAGATGAATACAGCTACGTCGCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203304 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSLKPRVVDFDETWNKLLTTIKAVVMLEYVERATWNDRFSDIYALCVAYPEPLGERLYTETKIFLENHVR
  HLHKRVLESEEQVLVMYHRYWEEYSKGADYMDCLYRYLNTQFIKKNKLTEADLQYGYGGVDMNEPLMEIG
  ELALDMWRKLMVEPLQAILIRMLLREIKNDRGGEDPNQKVIHGVINSFVHVEQYKKKFPLKFYQEIFESP
  FLTETGEYYKQEASNLLQESNCSQYMEKVLGRLKDEEIRCRKYLHPSSYTKVIHECQQRMVADHLQFLHA
  ECHNIIRQEKKNDMANMYVLLRAVSTGLPHMIQELQNHIHDEGLRATSNLTQENMPTLFVESVLEVHGKF
  VQLINTVLNGDQHFMSALDKALTSVVNYREPKSVCKAPELLAKYCDNLLKKSAKGMTENEVEDRLTSFIT
  VFKYIDDKDVFQKFYARMLAKRLIHGLSMSMDSEEAMINKLKQACGYEFTSKLHRMYTDMSVSADLNNKF
  NNFIKNQDTVIDLGISFQIYVLQAGAWPLTQAPSSTFAIPQELEKSVQMFELFYSQHFSGRKLTWLHYLC
  TGEVKMNYLGKPYVAMVTTYQMAVLLAFNNSETVSYKELQDSTQMNEKELTKTIKSLLDVKMINHDSEKE
  DIDAESSFSLNMNFSSKRTKFKITTSMQKDTPQEMEQTRSAVDEDRKMYLQAAIVRIMKARKVLRHNALI
  QEVISQSRARFNPSISMIKKCIEVLIDKQYIERSQASADEYSYVA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003591
• ORF Size: 2235 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003591.4
• RefSeq Size: 4238 bp
• RefSeq ORF: 2238 bp
• Locus ID: 8453
• UniProt ID: Q13617
• Cytogenetics: 10p11.21
• Domains: CULLIN
• Protein Families: Druggable Genome
• Protein Pathways: Pathways in cancer, Renal cell carcinoma, Ubiquitin mediated proteolysis
• MW: 87 kDa
• Gene Summary: Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346). May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).[UniProtKB/Swiss-Prot Function]