NIPSNAP3A (NM_015469) Human Tagged ORF Clone

CAT#: RC203264

NIPSNAP3A (Myc-DDK-tagged)-Human nipsnap homolog 3A (C. elegans) (NIPSNAP3A)

Product Images

Circular map for RC203264

• 

  Western blot validation of overexpression lysate (Cat# LY402439) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203264 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified NIPSNAP3A protein (Cat# TP303264). The protein was produced from HEK293T cells transfected with NIPSNAP3A cDNA clone (Cat# RC203264) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NIPSNAP3A
• Synonyms: HSPC299; NIPSNAP4; TASSC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203264 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCGTCCTCAGAAGCGCCCTGACTCGGGCGCTGGCCTCACGGACGCTGGCGCCTCAGATGTGCTCAT
  CTTTTGCTACGGGACCCAGACAATACGATGGAATATTCTATGAATTTCGTTCTTATTACCTTAAGCCCTC
  AAAGATGAATGAGTTCCTGGAAAATTTTGAGAAAAACGCTCATCTTCGGACAGCTCACTCTGAATTGGTT
  GGATACTGGAGTGTAGAATTTGGAGGCAGAATGAATACAGTGTTTCATATTTGGAAGTATGATAATTTTG
  CTCATCGAACTGAAGTTCGGAAAGCCTTGGCCAAAGATAAGGAATGGCAAGAACAATTCCTCATTCCAAA
  TTTGGCTCTCATTGATAAACAAGAGAGTGAGATTACTTATCTGGTACCATGGTGCAAATTAGAAAAACCT
  CCAAAAGAAGGAGTCTATGAACTGGCCACTTTTCAGATGAAACCTGGTGGGCCAGCTCTGTGGGGTGATG
  CATTTAAAAGGGCAGTTCATGCTCATGTCAATCTAGGCTACACAAAACTAGTTGGAGTGTTCCACACAGA
  GTACGGAGCACTCAACAGAGTTCATGTTCTTTGGTGGAATGAGAGTGCAGATAGTCGTGCAGCTGGGAGA
  CATAAGTCCCATGAGGATCCCAGAGTTGTGGCAGCTGTTCGGGAAAGTGTCAACTACCTAGTATCTCAGC
  AGAATATGCTTCTGATTCCTACATCGTTTTCACCACTGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203264 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLVLRSALTRALASRTLAPQMCSSFATGPRQYDGIFYEFRSYYLKPSKMNEFLENFEKNAHLRTAHSELV
  GYWSVEFGGRMNTVFHIWKYDNFAHRTEVRKALAKDKEWQEQFLIPNLALIDKQESEITYLVPWCKLEKP
  PKEGVYELATFQMKPGGPALWGDAFKRAVHAHVNLGYTKLVGVFHTEYGALNRVHVLWWNESADSRAAGR
  HKSHEDPRVVAAVRESVNYLVSQQNMLLIPTSFSPLK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015469
• ORF Size: 741 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015469.3
• RefSeq Size: 1664 bp
• RefSeq ORF: 744 bp
• Locus ID: 25934
• UniProt ID: Q9UFN0
• Cytogenetics: 9q31.1
• MW: 28.5 kDa
• Gene Summary: NIPSNAP3A belongs to a family of proteins with putative roles in vesicular transport (Buechler et al., 2004 [PubMed 15177564]).[supplied by OMIM, Mar 2008]