PCTP L (STARD10) (NM_006645) Human Tagged ORF Clone

CAT#: RC203228

STARD10 (Myc-DDK-tagged)-Human StAR-related lipid transfer (START) domain containing 10 (STARD10)

Product Images

Circular map for RC203228

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  Western blot validation of overexpression lysate (Cat# LY416482) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203228 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified STARD10 protein (Cat# TP303228). The protein was produced from HEK293T cells transfected with STARD10 cDNA clone (Cat# RC203228) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PCTP L
• Synonyms: CGI-52; NY-CO-28; PCTP2; SDCCAG28
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203228 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAGCTGGCGGCCTCTACAGAGCCCCAAGGGCCTCGGCCGGTCCTGGGCCGTGAGAGTGTCCAGG
  TGCCCGATGACCAAGACTTTCGCAGCTTCCGGTCAGAGTGTGAGGCTGAGGTGGGCTGGAACCTGACCTA
  TAGCAGGGCTGGGGTGTCTGTCTGGGTGCAGGCTGTGGAGATGGATCGGACGCTGCACAAGATCAAGTGC
  CGGATGGAGTGCTGTGATGTGCCAGCCGAGACACTCTACGACGTCCTACACGACATTGAGTACCGCAAGA
  AATGGGACAGCAACGTCATTGAGACTTTTGACATCGCCCGCTTGACAGTCAACGCTGACGTGGGCTATTA
  CTCCTGGAGGTGTCCCAAGCCCCTGAAGAACCGTGATGTCATCACCCTCCGCTCCTGGCTCCCCATGGGC
  GCTGATTACATCATTATGAACTACTCAGTCAAACATCCCAAATACCCACCTCGGAAAGACTTGGTCCGAG
  CTGTGTCCATCCAGACGGGCTACCTCATCCAGAGCACAGGGCCCAAGAGCTGCGTCATCACCTACCTGGC
  CCAGGTGGACCCCAAAGGCTCCTTACCCAAGTGGGTGGTGAATAAATCTTCTCAGTTCCTGGCTCCCAAG
  GCCATGAAGAAGATGTACAAGGCGTGCCTCAAGTACCCCGAGTGGAAACAGAAGCACCTGCCTCACTTCA
  AGCCGTGGCTGCACCCGGAGCAGAGCCCGTTGCCGAGCCTGGCGCTGTCGGAGCTGTCGGTGCAGCATGC
  GGACTCACTGGAGAACATCGACGAGAGCGCGGTGGCCGAGAGCAGAGAGGAGCGGATGGGCGGCGCGGGC
  GGCGAGGGCAGCGACGACGACACCTCGCTCACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203228 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEKLAASTEPQGPRPVLGRESVQVPDDQDFRSFRSECEAEVGWNLTYSRAGVSVWVQAVEMDRTLHKIKC
  RMECCDVPAETLYDVLHDIEYRKKWDSNVIETFDIARLTVNADVGYYSWRCPKPLKNRDVITLRSWLPMG
  ADYIIMNYSVKHPKYPPRKDLVRAVSIQTGYLIQSTGPKSCVITYLAQVDPKGSLPKWVVNKSSQFLAPK
  AMKKMYKACLKYPEWKQKHLPHFKPWLHPEQSPLPSLALSELSVQHADSLENIDESAVAESREERMGGAG
  GEGSDDDTSLT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006645
• ORF Size: 873 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006645.2, NP_006636.2
• RefSeq Size: 1988 bp
• RefSeq ORF: 876 bp
• Locus ID: 10809
• UniProt ID: Q9Y365
• Cytogenetics: 11q13.4
• Domains: START
• MW: 33 kDa
• Gene Summary: May play metabolic roles in sperm maturation or fertilization (By similarity). Phospholipid transfer protein that preferentially selects lipid species containing a palmitoyl or stearoyl chain on the sn-1 and an unsaturated fatty acyl chain (18:1 or 18:2) on the sn-2 position. Able to transfer phosphatidylcholine (PC) and phosphatidyetanolamline (PE) between membranes.[UniProtKB/Swiss-Prot Function]