Apc5 (ANAPC5) (NM_016237) Human Tagged ORF Clone

CAT#: RC203030

ANAPC5 (Myc-DDK-tagged)-Human anaphase promoting complex subunit 5 (ANAPC5), transcript variant 1

Product Images

Circular map for RC203030

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  Western blot validation of overexpression lysate (Cat# LY402524) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203030 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ANAPC5 protein (Cat# TP303030). The protein was produced from HEK293T cells transfected with ANAPC5 cDNA clone (Cat# RC203030) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Apc5
• Synonyms: APC5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203030 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGCGTCCACGAGAGCCTCTACTTCAATCCCATGATGACCAATGGGGTTGTGCACGCCAATGTGT
  TCGGCATCAAGGACTGGGTGACGCCGTACAAGATCGCGGTGCTGGTGCTGCTGAACGAGATGAGCCGCAC
  AGGCGAGGGCGCCGTCAGCCTCATGGAGCGGCGGAGGCTCAACCAGCTGCTCCTGCCCCTGCTGCAGGGC
  CCAGATATTACACTGTCAAAACTTTACAAGTTAATTGAAGAGTCTTGTCCACAGCTGGCAAATTCAGTGC
  AGATCAGAATCAAACTGATGGCTGAAGGCGAGTTGAAGGATATGGAACAGTTTTTTGATGACCTTTCAGA
  TTCTTTCTCTGGAACTGAACCAGAGGTTCACAAAACAAGTGTAGTAGGTTTGTTTCTGCGTCACATGATC
  TTGGCCTACAGTAAGCTTTCTTTCAGCCAAGTGTTTAAACTGTACACTGCCCTTCAGCAGTACTTCCAGA
  ATGGTGAGAAAAAGACAGTGGAGGATGCTGATATGGAACTGACCAGTAGAGATGAGGGTGAAAGAAAAAT
  GGAAAAAGAAGAACTTGATGTATCTGTAAGAGAAGAGGAGGTATCTTGCAGTGGGCCTCTGTCCCAAAAA
  CAAGCAGAATTTTTTCTTTCTCAACAGGCTTCTTTGCTAAAGAATGATGAGACTAAGGCCCTCACTCCAG
  CTTCCTTGCAGAAGGAATTAAACAATTTGTTGAAATTTAATCCTGATTTTGCTGAAGCGCATTATCTCAG
  CTACTTAAACAACCTCCGTGTCCAAGATGTTTTCAGTTCAACACACAGTCTCCTCCATTATTTTGATCGT
  CTGATTCTTACCGGAGCCGAAAGCAAAAGTAATGGGGAAGAGGGCTATGGCCGGAGCTTGAGATACGCCG
  CTCTGAATCTTGCCGCCCTGCACTGCCGCTTCGGTCACTATCAACAGGCAGAGCTCGCCCTGCAGGAGGC
  AATTAGGATTGCCCAGGAGTCCAACGATCACGTGTGTCTCCAGCACTGTTTGAGCTGGCTTTATGTGCTG
  GGGCAGAAGAGATCCGATAGCTATGTTCTGCTGGAGCATTCTGTGAAGAAGGCAGTACATTTTGGGTTAC
  CGTACCTCGCCTCCCTGGGAATACAGTCCCTTGTTCAACAGAGAGCTTTTGCTGGGAAGACGGCAAACAA
  GCTGATGGATGCCCTAAAGGACTCCGACCTCCTGCACTGGAAACACAGCCTGTCAGAGCTCATCGATATC
  AGCATCGCACAGAAAACGGCCATCTGGAGGCTGTATGGCCGCAGCACCATGGCACTGCAACAGGCCCAGA
  TGTTGCTGAGCATGAACAGCCTGGAGGCGGTGAATGCGGGCGTGCAGCAGAACAACACAGAGTCCTTTGC
  TGTCGCACTCTGCCACCTCGCAGAGCTACACGCGGAGCAGGGCTGTTTTGCTGCAGCTTCTGAAGTGTTA
  AAGCACTTGAAGGAACGATTTCCGCCTAATAGTCAGCACGCCCAGTTATGGATGTTATGTGATCAAAAAA
  TACAGTTTGACAGAGCAATGAATGATGGCAAATATCATTTGGCTGATTCACTTGTTACAGGAATCACAGC
  TCTCAATAGCATAGAGGGTGTTTATAGGAAAGCGGTTGTATTACAAGCTCAGAACCAAATGTCAGAGGCA
  CATAAGCTTTTACAAAAATTGTTGGTTCATTGTCAGAAACTGAAGAACACAGAAATGGTGATCAGTGTCC
  TACTGTCCGTGGCAGAGCTGTACTGGCGATCTTCCTCCCCTACCATCGCGCTGCCCATGCTCCTGCAGGC
  TCTGGCCCTCTCCAAGGAGTACCGGTTACAGTACTTGGCCTCTGAAACAGTGCTGAACTTGGCTTTTGCG
  CAGCTCATTCTTGGAATCCCAGAACAGGCCTTAAGTCTTCTCCACATGGCCATCGAGCCCATCTTGGCTG
  ACGGGGCTATCCTGGACAAAGGTCGTGCCATGTTCTTAGTGGCCAAGTGCCAGGTGGCTTCAGCAGCTTC
  CTACGATCAGCCGAAGAAAGCAGAAGCTCTGGAGGCTGCCATCGAGAACCTCAATGAAGCCAAGAACTAT
  TTTGCAAAGGTTGACTGCAAAGAGCGCATCAGGGACGTCGTTTACTTCCAGGCCAGACTCTACCATACCC
  TGGGGAAGACCCAGGAGAGGAACCGGTGTGCGATGCTCTTCCGGCAGCTGCATCAGGAGCTGCCCTCTCA
  TGGGGTACCCTTGATAAACCATCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203030 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASVHESLYFNPMMTNGVVHANVFGIKDWVTPYKIAVLVLLNEMSRTGEGAVSLMERRRLNQLLLPLLQG
  PDITLSKLYKLIEESCPQLANSVQIRIKLMAEGELKDMEQFFDDLSDSFSGTEPEVHKTSVVGLFLRHMI
  LAYSKLSFSQVFKLYTALQQYFQNGEKKTVEDADMELTSRDEGERKMEKEELDVSVREEEVSCSGPLSQK
  QAEFFLSQQASLLKNDETKALTPASLQKELNNLLKFNPDFAEAHYLSYLNNLRVQDVFSSTHSLLHYFDR
  LILTGAESKSNGEEGYGRSLRYAALNLAALHCRFGHYQQAELALQEAIRIAQESNDHVCLQHCLSWLYVL
  GQKRSDSYVLLEHSVKKAVHFGLPYLASLGIQSLVQQRAFAGKTANKLMDALKDSDLLHWKHSLSELIDI
  SIAQKTAIWRLYGRSTMALQQAQMLLSMNSLEAVNAGVQQNNTESFAVALCHLAELHAEQGCFAAASEVL
  KHLKERFPPNSQHAQLWMLCDQKIQFDRAMNDGKYHLADSLVTGITALNSIEGVYRKAVVLQAQNQMSEA
  HKLLQKLLVHCQKLKNTEMVISVLLSVAELYWRSSSPTIALPMLLQALALSKEYRLQYLASETVLNLAFA
  QLILGIPEQALSLLHMAIEPILADGAILDKGRAMFLVAKCQVASAASYDQPKKAEALEAAIENLNEAKNY
  FAKVDCKERIRDVVYFQARLYHTLGKTQERNRCAMLFRQLHQELPSHGVPLINHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016237
• ORF Size: 2265 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016237.3, NP_057321.2
• RefSeq Size: 2625 bp
• RefSeq ORF: 2268 bp
• Locus ID: 51433
• UniProt ID: Q9UJX4
• Cytogenetics: 12q24.31
• Domains: TPR
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis
• MW: 85.1 kDa
• Gene Summary: This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a. [provided by RefSeq, Nov 2008]