PLCD4 (NM_032726) Human Tagged ORF Clone

CAT#: RC202935

PLCD4 (Myc-DDK-tagged)-Human phospholipase C, delta 4 (PLCD4)

Product Images

Circular map for RC202935

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  Western blot validation of overexpression lysate (Cat# LY409963) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202935 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PLCD4 protein (Cat# TP302935). The protein was produced from HEK293T cells transfected with PLCD4 cDNA clone (Cat# RC202935) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PLCD4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202935 representing NM_032726.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGTCCCTGCTGCAAGACCAGCTGACCACTGATCAGGACTTGCTGCTGATGCAGGAAGGCATGTCG
  ATGCGCAAGGTGAGGTCCAAAAGCTGGAAGAAGCTAAGATACTTCAGACTTCAGAATGACGGCATGACA
  GTCTGGCATGCACGGCAGGCCAGGGGCAGTGCCAAGCCCAGCTTCTCAATCTCTGATGTGGAGACAATA
  CGTAATGGCCATGATTCCGAGTTGCTGCGTAGCCTGGCAGAGGAGCTCCCCCTGGAGCAGGGCTTCACC
  ATTGTCTTCCATGGCCGCCGCTCCAACCTGGACCTGATGGCCAACAGTGTTGAGGAGGCCCAGATATGG
  ATGCGAGGGCTCCAGCTGTTGGTGGATCTTGTCACCAGCATGGACCATCAGGAGCGCCTGGACCAATGG
  CTGAGCGATTGGTTTCAACGTGGAGACAAAAATCAGGATGGTAAGATGAGTTTCCAAGAAGTTCAGCGG
  TTATTGCACCTAATGAATGTGGAAATGGACCAAGAATATGCCTTCAGTCTTTTTCAGGCAGCAGACACG
  TCCCAGTCTGGAACCCTGGAAGGAGAAGAATTCGTACAGTTCTATAAGGCATTGACTAAACGTGCTGAG
  GTGCAGGAACTGTTTGAAAGTTTTTCAGCTGATGGGCAGAAGCTGACTCTGCTGGAATTTTTGGATTTC
  CTCCAAGAGGAGCAGAAGGAGAGAGACTGCACCTCTGAGCTTGCTCTGGAACTCATTGACCGCTATGAA
  CCTTCAGACAGTGGCAAACTGCGGCATGTGCTGAGTATGGATGGCTTCCTCAGCTACCTCTGCTCTAAG
  GATGGAGACATCTTCAACCCAGCCTGCCTCCCCATCTATCAGGATATGACTCAACCCCTGAACCACTAC
  TTCATCTGCTCTTCTCATAACACCTACCTAGTGGGGGACCAGCTTTGCGGCCAGAGCAGCGTCGAGGGA
  TATATACGGGCCCTGAAGCGGGGGTGCCGCTGCGTGGAGGTGGATGTATGGGATGGACCTAGCGGGGAA
  CCTGTCGTTTACCACGGACACACCCTGACCTCCCGCATCCTGTTCAAAGATGTCGTGGCCACAGTAGCA
  CAGTATGCCTTCCAGACATCAGACTACCCAGTCATCTTGTCCCTGGAGACCCACTGCAGCTGGGAGCAG
  CAGCAGACCATGGCCCGTCATCTGACTGAGATCCTGGGGGAGCAGCTGCTGAGCACCACCTTGGATGGG
  GTGCTGCCCACTCAGCTGCCCTCGCCTGAGGAGCTTCGGAGGAAGATCCTGGTGAAGGGGAAGAAGTTA
  ACACTTGAGGAAGACCTGGAATATGAGGAAGAGGAAGCAGAACCTGAGTTGGAAGAGTCAGAATTGGCG
  CTGGAGTCCCAGTTTGAGACTGAGCCTGAGCCCCAGGAGCAGAACCTTCAGAATAAGGACAAAAAGAAG
  AAATCCAAGCCCATCTTGTGTCCAGCCCTCTCTTCCCTGGTTATCTACTTGAAGTCTGTCTCATTCCGC
  AGCTTCACACATTCAAAGGAGCACTACCACTTCTACGAGATATCATCTTTCTCTGAAACCAAGGCCAAG
  CGCCTCATCAAGGAGGCTGGCAATGAGTTTGTGCAGCACAATACTTGGCAGTTAAGCCGTGTGTATCCC
  AGCGGCCTGAGGACAGACTCTTCCAACTACAACCCCCAGGAACTCTGGAATGCAGGCTGCCAGATGGTG
  GCCATGAATATGCAGACTGCAGGGCTTGAAATGGACATCTGTGATGGGCATTTCCGCCAGAATGGCGGC
  TGTGGCTATGTGCTGAAGCCAGACTTCCTGCGTGATATCCAGAGTTCTTTCCACCCTGAGAAGCCCATC
  AGCCCTTTCAAAGCCCAGACTCTCTTAATCCAGGTGATCAGCGGTCAGCAACTCCCCAAAGTGGACAAG
  ACCAAAGAGGGGTCCATTGTGGATCCACTGGTGAAAGTGCAGATCTTTGGCGTTCGTCTAGACACAGCA
  CGGCAGGAGACCAACTATGTGGAGAACAATGGTTTTAATCCATACTGGGGGCAGACACTATGTTTCCGG
  GTGCTGGTGCCTGAACTTGCCATGCTGCGTTTTGTGGTAATGGATTATGACTGGAAATCCCGAAATGAC
  TTTATTGGTCAGTACACCCTGCCTTGGACCTGCATGCAACAAGGTTACCGCCACATTCACCTGCTGTCC
  AAAGATGGCATCAGCCTCCGCCCAGCTTCCATCTTTGTGTATATCTGCATCCAGGAAGGCCTGGAGGGG
  GATGAGTCC
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  >Peptide sequence encoded by RC202935
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MASLLQDQLTTDQDLLLMQEGMSMRKVRSKSWKKLRYFRLQNDGMTVWHARQARGSAKPSFSISDVETI
  RNGHDSELLRSLAEELPLEQGFTIVFHGRRSNLDLMANSVEEAQIWMRGLQLLVDLVTSMDHQERLDQW
  LSDWFQRGDKNQDGKMSFQEVQRLLHLMNVEMDQEYAFSLFQAADTSQSGTLEGEEFVQFYKALTKRAE
  VQELFESFSADGQKLTLLEFLDFLQEEQKERDCTSELALELIDRYEPSDSGKLRHVLSMDGFLSYLCSK
  DGDIFNPACLPIYQDMTQPLNHYFICSSHNTYLVGDQLCGQSSVEGYIRALKRGCRCVEVDVWDGPSGE
  PVVYHGHTLTSRILFKDVVATVAQYAFQTSDYPVILSLETHCSWEQQQTMARHLTEILGEQLLSTTLDG
  VLPTQLPSPEELRRKILVKGKKLTLEEDLEYEEEEAEPELEESELALESQFETEPEPQEQNLQNKDKKK
  KSKPILCPALSSLVIYLKSVSFRSFTHSKEHYHFYEISSFSETKAKRLIKEAGNEFVQHNTWQLSRVYP
  SGLRTDSSNYNPQELWNAGCQMVAMNMQTAGLEMDICDGHFRQNGGCGYVLKPDFLRDIQSSFHPEKPI
  SPFKAQTLLIQVISGQQLPKVDKTKEGSIVDPLVKVQIFGVRLDTARQETNYVENNGFNPYWGQTLCFR
  VLVPELAMLRFVVMDYDWKSRNDFIGQYTLPWTCMQQGYRHIHLLSKDGISLRPASIFVYICIQEGLEG
  DES
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
  Recombinant protein using RC202935 also available, TP302935
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032726
• ORF Size: 2286 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 3237 bp
• RefSeq ORF: 2289 bp
• Locus ID: 84812
• UniProt ID: Q9BRC7
• Cytogenetics: 2q35
• Domains: C2, PI-PLC-X, PI-PLC-Y, PH, EFh
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• MW: 87.6 kDa
• Gene Summary: This gene encodes a member of the delta class of phospholipase C enzymes. Phospholipase C enzymes play a critical role in many cellular processes by hydrolyzing phosphatidylinositol 4,5-bisphosphate into two intracellular second messengers, inositol 1,4,5-trisphosphate and diacylglycerol. Expression of this gene may be a marker for cancer. [provided by RefSeq, Jan 2011]