Metallothionein (MT2A) (NM_005953) Human Tagged ORF Clone
MT2A (Myc-DDK-tagged)-Human metallothionein 2A (MT2A)
|Type||Human Tagged ORF Clone|
|Synonyms||MT-2; MT-II; MT2|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC202748 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC202748 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||183 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||466 bp|
|RefSeq ORF||186 bp|
|Gene Summary||This gene is a member of the metallothionein family of genes. Proteins encoded by this gene family are low in molecular weight, are cysteine-rich, lack aromatic residues, and bind divalent heavy metal ions, altering the intracellular concentration of heavy metals in the cell. These proteins act as anti-oxidants, protect against hydroxyl free radicals, are important in homeostatic control of metal in the cell, and play a role in detoxification of heavy metals. The encoded protein interacts with the protein encoded by the homeobox containing 1 gene in some cell types, controlling intracellular zinc levels, affecting apoptotic and autophagy pathways. Some polymorphisms in this gene are associated with an increased risk of cancer. [provided by RefSeq, Sep 2017]|
|cDNA Clone Resources|
|RC202748L1||Lenti ORF clone of Human metallothionein 2A (MT2A), Myc-DDK-tagged||
|RC202748L2||Lenti ORF clone of Human metallothionein 2A (MT2A), mGFP tagged||
|RC202748L3||Lenti ORF clone of Human metallothionein 2A (MT2A), Myc-DDK-tagged||
|RC202748L4||Lenti ORF clone of Human metallothionein 2A (MT2A), mGFP tagged||
|RG202748||MT2A (GFP-tagged) - Human metallothionein 2A (MT2A)||
|SC125000||MT2A (untagged)-Human metallothionein 2A (MT2A)||