NDUFAF7 (NM_144736) Human Tagged ORF Clone

CAT#: RC202529

NDUFAF7 (Myc-DDK-tagged)-Human chromosome 2 open reading frame 56 (C2orf56), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Images

Circular map for RC202529

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY C2orf56 (Cat# RC202529, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C2orf56(Cat# TA502995). Positive lysates LY403408 (100ug) and LC403408 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY403408) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202529 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified NDUFAF7 protein (Cat# TP302529). The protein was produced from HEK293T cells transfected with NDUFAF7 cDNA clone (Cat# RC202529) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NDUFAF7
• Synonyms: C2orf56; MidA; PRO1853
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202529 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGTACTGCTGAGGTCAGGTTTGGGGCCGTTGTGTGCCGTGGCGCGCGCAGCCATTCCTTTTATTT
  GGAGAGGGAAATACTTCAGCTCCGGGAATGAGCCTGCAGAAAACCCGGTGACGCCGATGCTGCGGCATCT
  TATGTACAAAATAAAGTCTACTGGTCCCATCACTGTGGCCGAGTACATGAAGGAGGTGTTGACTAATCCA
  GCCAAGGGTTATTATGTGTACCGTGACATGCTAGGCGAAAAAGGAGATTTCATTACTTCACCTGAAATAA
  GTCAAATCTTTGGGGAGCTACTAGGTATATGGTTCATTAGTGAATGGATGGCCACTGGAAAAAGCACAGC
  TTTCCAGCTGGTGGAACTGGGCCCAGGTAGGGGAACCCTCGTGGGAGATATTTTGAGGGTGTTCACTCAA
  CTTGGATCTGTGCTGAAAAATTGTGACATTTCAGTACATCTGGTAGAGGTAAGCCAAAAATTAAGTGAGA
  TTCAAGCATTGACACTGACTAAAGAGAAGGTCCCGTTAGAGCGAAATGCTGGATCCCCAGTGTATATGAA
  AGGTGTCACTAAGTCTGGGATTCCAATTTCCTGGTACCGAGATCTGCACGATGTTCCAAAAGGGTACAGC
  TTTTATCTTGCACATGAATTTTTTGATGTTCTTCCTGTGCATAAATTTCAGAAAACACCACAGGGATGGC
  GAGAAGTATTTGTTGACATTGATCCACAGGTTTCTGATAAACTGAGGTTTGTTTTGGCACCTTCTGCCAC
  CCCAGCAGAAGCCTTCATACAACATGACGAAACAAGGGATCATGTTGAAGTGTGTCCTGATGCTGGTGTT
  ATCATCGAGGAACTTTCTCAACGCATTGCATTAACTGGAGGTGCTGCACTGGTTGCTGATTATGGTCATG
  ATGGAACAAAGACAGATACCTTCAGAGGGTTTTGCGACCACAAGCTTCATGATGTCTTAATTGCCCCAGG
  AACAGCAGATCTAACAGCTGATGTGGACTTCAGTTATTTGCGAAGAATGGCACAGGGAAAAGTAGCCTCT
  CTGGGCCCAATAAAACAACACACATTTTTAAAAAATATGGGTATTGATGTCCGGCTGAAGGTTCTTTTAG
  ATAAATCAAATGAGCCATCAGTGAGGCAGCAGTTACTTCAAGGATATGATATGTTAATGAATCCAAAGAA
  GATGGGAGAGAGATTTAACTTTTTTGCCTTGCTACCTCATCAGAGACTTCAAGGTGGAAGATATCAGAGG
  AATGCACGTCAGTCAAAACCCTTTGCATCCGTTGTAGCTGGGTTTAGTGAACTTGCTTGGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202529 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSVLLRSGLGPLCAVARAAIPFIWRGKYFSSGNEPAENPVTPMLRHLMYKIKSTGPITVAEYMKEVLTNP
  AKGYYVYRDMLGEKGDFITSPEISQIFGELLGIWFISEWMATGKSTAFQLVELGPGRGTLVGDILRVFTQ
  LGSVLKNCDISVHLVEVSQKLSEIQALTLTKEKVPLERNAGSPVYMKGVTKSGIPISWYRDLHDVPKGYS
  FYLAHEFFDVLPVHKFQKTPQGWREVFVDIDPQVSDKLRFVLAPSATPAEAFIQHDETRDHVEVCPDAGV
  IIEELSQRIALTGGAALVADYGHDGTKTDTFRGFCDHKLHDVLIAPGTADLTADVDFSYLRRMAQGKVAS
  LGPIKQHTFLKNMGIDVRLKVLLDKSNEPSVRQQLLQGYDMLMNPKKMGERFNFFALLPHQRLQGGRYQR
  NARQSKPFASVVAGFSELAWQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144736
• ORF Size: 1323 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144736.5
• RefSeq Size: 2221 bp
• RefSeq ORF: 1326 bp
• Locus ID: 55471
• UniProt ID: Q7L592
• Cytogenetics: 2p22.2
• Domains: DUF185
• MW: 49.2 kDa
• Gene Summary: This gene encodes an assembly factor protein which helps in the assembly and stabilization of Complex I, a large multi-subunit enzyme in the mitochondrial respiratory chain. Complex I is involved in several physiological activities in the cell, including metabolite transport and ATP synthesis. The encoded protein is a methyltransferase which methylates Arg85 of a subunit of Complex I in the early stages of its assembly. A pseudogene related to this gene is located on chromosome 8. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]