HOOK1 (NM_015888) Human Tagged ORF Clone

CAT#: RC202517

HOOK1 (Myc-DDK-tagged)-Human hook homolog 1 (Drosophila) (HOOK1)

Product Images

Circular map for RC202517

• 

  Western blot validation of overexpression lysate (Cat# LY402472) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202517 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified HOOK1 protein (Cat# TP302517). The protein was produced from HEK293T cells transfected with HOOK1 cDNA clone (Cat# RC202517) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HOOK1
• Synonyms: HK1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202517 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGACGCAGCCGCCGCCGCAGCCTAAGCTGCCCCTGTGCGACAGCCTCATGATCTGGCTGCAGA
  CATTCAATACTGCCTCACCTTGTCAAGATGTCAAACAGCTGACTAGTGGAGTTGCCATGGCACAAGTTCT
  TCATCAAATTGATGCAGCTTGGTTTAACGAATCTTGGTTAAGCCGAATTAAAGAGGATGTTGGGGACAAC
  TGGAGAATAAAGGCCAGTAATGTAAAGAAGGTCCTTCAAGGAATTATGAGTTATTATCATGAGTTTTTGG
  GGCAGCAGATTTCAGAAGCACTTATCCCTGATTTAAACCAAATAACCGAATGTTCAGATCCAGTGGAGCT
  TGGGAGGTTGCTCCAGCTTATTTTAGGTTGTGCGATCAACTGTGAAAAGAAGCAAGAACATATTCAAAAT
  ATAATGACACTGGAAGAGTCTGTTCAACATGTGGTCATGACTGCTATTCAAGAGTTGATGAGTAAAGAAA
  TATTGAGCTCTCCTCCAAATGATGCTGTTGGAGAATTGGAGCAACAGCTTAAAAGAGCCTTGGAAGAACT
  TCAGGAAGCACTAGCAGAAAAAGAAGAGCTGAGGCAAAGATGTGAAGAATTGGATATGCAGGTGACTACA
  CTTCAAGATGAAAAGAATTCACTGGTTTCTGAAAATGAGATGATGAATGAAAAACTTGACCAGTTGGATG
  GCTCTTTTGATGATCCAAACACAGTGGTTGCAAAAAAGTATTTTCATGCACAATTACAACTAGAACAATT
  ACAGGAAGAAAACTTCAGGCTTGAAGCTGCAAAAGATGATTACCGTGTTCACTGTGAAGAACTTGAAAAG
  CAGCTAATCGAATTCCAGCATAGGAATGATGAATTGACTAGTCTTGCAGAAGAAACAAGAGCCCTGAAAG
  ATGAAATAGATGTTCTTAGGGCTACCTCTGATAAAGCAAATAAACTGGAGTCAACAGTTGAGATATATCG
  TCAGAAGCTACAAGATCTGAATGACCTTCGCAAGCAGGTGAAAACTTTACAGGAAACCAACATGATGTAT
  ATGCATAATACAGTCAGCTTAGAAGAAGAATTAAAAAAAGCAAATGCAGCACGTACACAATTAGAAACAT
  ACAAAAGGCAGGTTCAAGATCTTCATGTTAAACTTTCCTCCGAATCCAAGAGGGCAGACACACTAGCGTT
  TGAAATGAAGCGGCTTGAAGAAAAACATGAAGCTTTACTTAAGGAAAAAGAGAGACTAATTGAGCAGCGT
  GATACTTTGAAAGAAACAAATGAAGAGCTTCGATGTTCACAAGTACAACAGGACCACCTAAACCAAACAG
  ATGCATCTGCTACAAAAAGTTATGAGAATCTTGCTGCTGAGATTATGCCAGTGGAATATAGGGAGGTGTT
  TATTCGACTGCAACATGAAAATAAGATGCTTCGCTTACAGCAAGAAGGCTCTGAGAATGAACGTATTGAG
  GAACTTCAGGAGCAGCTAGAACAGAAACACCGTAAAATGAATGAACTGGAAACTGAGCAGAGGCTGAGCA
  AAGAGCGTATTAGAGAATTGCAGCAGCAGATTGAGGACCTCCAGAAATCTTTACAGGAACAAGGTTCCAA
  GTCTGAAGGCGAAAGTTCCAGCAAATTAAAGCAGAAGTTGGAAGCTCATATGGAAAAACTCACAGAGGTC
  CATGAAGAATTACAGAAGAAACAAGAACTCATTGAAGATCTTCAGCCAGATATAAATCAAAATGTACAAA
  AGATCAATGAACTTGAAGCTGCTCTTCAGAAGAAAGATGAAGATATGAAAGCAATGGAGGAAAGATATAA
  AATGTACTTGGAGAAAGCCAGAAATGTAATAAAAACTTTGGATCCCAAGTTAAATCCAGCATCAGCTGAA
  ATAATGCTACTAAGAAAGCAGTTGGCAGAGAAAGAGAGAAGAATTGAGATTCTGGAGAGTGAATGCAAAG
  TAGCAAAATTCCGTGATTATGAAGAAAAACTCATTGTTTCTGCGTGGTATAATAAGAGTCTAGCATTCCA
  GAAACTGGGGATGGAATCTAGACTTGTGAGCGGCGGTGGTGCCTGCAGTGACACTGGTGCGTGCACTCCT
  GCGCGGTCTTTCTTAGCGCAGCAACGGCACATCACCAACACCAGAAGAAATCTCTCTGTTAAAGTCCCTG
  CTACAACATCTGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202517 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEETQPPPQPKLPLCDSLMIWLQTFNTASPCQDVKQLTSGVAMAQVLHQIDAAWFNESWLSRIKEDVGDN
  WRIKASNVKKVLQGIMSYYHEFLGQQISEALIPDLNQITECSDPVELGRLLQLILGCAINCEKKQEHIQN
  IMTLEESVQHVVMTAIQELMSKEILSSPPNDAVGELEQQLKRALEELQEALAEKEELRQRCEELDMQVTT
  LQDEKNSLVSENEMMNEKLDQLDGSFDDPNTVVAKKYFHAQLQLEQLQEENFRLEAAKDDYRVHCEELEK
  QLIEFQHRNDELTSLAEETRALKDEIDVLRATSDKANKLESTVEIYRQKLQDLNDLRKQVKTLQETNMMY
  MHNTVSLEEELKKANAARTQLETYKRQVQDLHVKLSSESKRADTLAFEMKRLEEKHEALLKEKERLIEQR
  DTLKETNEELRCSQVQQDHLNQTDASATKSYENLAAEIMPVEYREVFIRLQHENKMLRLQQEGSENERIE
  ELQEQLEQKHRKMNELETEQRLSKERIRELQQQIEDLQKSLQEQGSKSEGESSSKLKQKLEAHMEKLTEV
  HEELQKKQELIEDLQPDINQNVQKINELEAALQKKDEDMKAMEERYKMYLEKARNVIKTLDPKLNPASAE
  IMLLRKQLAEKERRIEILESECKVAKFRDYEEKLIVSAWYNKSLAFQKLGMESRLVSGGGACSDTGACTP
  ARSFLAQQRHITNTRRNLSVKVPATTSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015888
• ORF Size: 2184 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015888.6
• RefSeq Size: 5865 bp
• RefSeq ORF: 2187 bp
• Locus ID: 51361
• UniProt ID: Q9UJC3
• Cytogenetics: 1p32.1
• MW: 84.6 kDa
• Gene Summary: This gene encodes a member of the hook family of coiled-coil proteins, which bind to microtubules and organelles through their N- and C-terminal domains, respectively. The encoded protein localizes to discrete punctuate subcellular structures, and interacts with several members of the Rab GTPase family involved in endocytosis. It is thought to link endocytic membrane trafficking to the microtubule cytoskeleton. Several alternatively spliced transcript variants have been identified, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]