GPSN2 (TECR) (NM_138501) Human Tagged ORF Clone

CAT#: RC202503

TECR (Myc-DDK-tagged)-Human trans-2,3-enoyl-CoA reductase (TECR), transcript variant 1

Product Images

Circular map for RC202503

• 

  Western blot validation of overexpression lysate (Cat# LY403358) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202503 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GPSN2
• Synonyms: GPSN2; MRT14; SC2; TER
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202503 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCATTACGAGGTGGAGATTCTGGACGCAAAGACAAGGGAGAAGCTGTGTTTCTTGGACAAGGTGG
  AGCCCCACGCCACCATTGCGGAGATCAAGAACCTCTTCACTAAGACCCATCCGCAGTGGTACCCCGCCCG
  CCAGTCCCTCCGCCTGGACCCCAAGGGCAAGTCCCTGAAGGATGAGGATGTTCTGCAGAAGCTGCCCGTG
  GGCACCACGGCCACACTGTACTTCCGGGACCTGGGGGCCCAGATCAGCTGGGTGACGGTCTTCCTAACAG
  AGTACGCGGGGCCCCTTTTCATCTACCTGCTCTTCTACTTCCGAGTGCCCTTCATCTATGGCCACAAATA
  TGACTTTACGTCCAGTCGGCATACAGTGGTGCACCTCGCCTGCATCTGTCACTCATTCCACTACATCAAG
  CGCCTGCTGGAGACGCTCTTCGTGCACCGCTTCTCCCATGGCACTATGCCTTTGCGCAACATCTTCAAGA
  ACTGCACCTACTACTGGGGCTTCGCCGCGTGGATGGCCTATTACATCAATCACCCTCTCTACACTCCCCC
  TACCTACGGAGCTCAGCAGGTGAAACTGGCGCTCGCCATCTTTGTGATCTGCCAGCTCGGCAACTTCTCC
  ATCCACATGGCCCTGCGGGACCTGCGGCCCGCTGGGTCCAAGACGCGGAAGATCCCATACCCCACCAAGA
  ACCCCTTCACGTGGCTCTTCCTGCTGGTGTCCTGCCCCAACTACACCTACGAGGTGGGGTCCTGGATCGG
  TTTCGCCATCATGACGCAGTGTCTCCCAGTGGCCCTGTTCTCCCTGGTGGGCTTCACCCAGATGACCATC
  TGGGCCAAGGGCAAGCACCGCAGCTACCTGAAGGAGTTCCGGGACTACCCGCCCCTGCGCATGCCCATCA
  TCCCCTTCCTGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202503 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKHYEVEILDAKTREKLCFLDKVEPHATIAEIKNLFTKTHPQWYPARQSLRLDPKGKSLKDEDVLQKLPV
  GTTATLYFRDLGAQISWVTVFLTEYAGPLFIYLLFYFRVPFIYGHKYDFTSSRHTVVHLACICHSFHYIK
  RLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHPLYTPPTYGAQQVKLALAIFVICQLGNFS
  IHMALRDLRPAGSKTRKIPYPTKNPFTWLFLLVSCPNYTYEVGSWIGFAIMTQCLPVALFSLVGFTQMTI
  WAKGKHRSYLKEFRDYPPLRMPIIPFLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138501
• ORF Size: 924 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138501.6
• RefSeq Size: 1180 bp
• RefSeq ORF: 927 bp
• Locus ID: 9524
• UniProt ID: Q9NZ01
• Cytogenetics: 19p13.12
• Domains: Steroid_dh
• Protein Families: Transmembrane
• Protein Pathways: Biosynthesis of unsaturated fatty acids
• MW: 36 kDa
• Gene Summary: This gene encodes a multi-pass membrane protein that resides in the endoplasmic reticulum, and belongs to the steroid 5-alpha reductase family. The elongation of microsomal long and very long chain fatty acid consists of 4 sequential reactions. This protein catalyzes the final step, reducing trans-2,3-enoyl-CoA to saturated acyl-CoA. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Apr 2011]