TMEM44 (NM_001011655) Human Tagged ORF Clone

CAT#: RC202358

TMEM44 (Myc-DDK-tagged)-Human transmembrane protein 44 (TMEM44), transcript variant 2

Product Images

Circular map for RC202358

• 

  Western blot validation of overexpression lysate (Cat# LY423283) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202358 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TMEM44
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202358 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGAGGCGCCCAGCCCCGCGCCCGCGCTCTGGGACTGGGACTACCTGGACCGCTGCTTCGCCCGCA
  ACCGCGTCTGCATCTCCTTCGGCCTGTGGATCTGCGCCTCCTCCTGCTGGATCGCCGCCCACGCGCTGCT
  TCTCTATCTGAGATGTGCACAGAAACCCAGACAGGACCAGTCGGCACTGTGTGCTGCATGCTGCCTCCTG
  ACCAGTCTGTGTGACACCGTCGGGGCTCTTCTGGCCAGACAGCTCACAATCCAGGTTTTCACTGGTGCCT
  ACCTAGCAGCTATTGACTTAGTGAACTTTATGTTCATTCTCTTCCCAGTCTGTGGATCCAAATTCAAGTC
  TAATTCAGATCGGGAAGCCCGAGAGAGGAAGAGGAGGCGGCAGCTCAGGGCCAGTGTGTTTGCCCTGGCC
  CTGCCGCTGAGCCTGGGCCCGTGCTGGGCTCTGTGGGTTGCTGTCCCGAAGGCTTCAGCCACCATCCGGG
  GGCCACAGCGGAGGCTGCTAGCGAGCCTGCTGCAGGAAAATACTGAGATCCTCGGCTACCTGCTGGGTAG
  CGTTGCTGCCTTTGGCTCCTGGGCTTCTCGGATCCCCCCTCTCTCCAGAATTTGCCGGGGGAAGACATTT
  CCCTCCATCCACCTGTGGACCCGGCTCCTGTCGGCCCTGGCTGGCCTCCTCTATGCCTCGGCCATTGTGG
  CCCACGACCGGCACCCTGAGTACCTGCTGCGGGCCACACCCTGGTTCCTGACCTCCCTCGGCCGTGCGGC
  ACTGGACCTCGCTATTATTTTCCTTTCGTGTGTGATGAAGAGCAAGATGAGACAGGCCTTAGGATTTGCC
  AAGGAAGCCAGAGAGAGCCCTGACACCCAAGCCCTTTTGACCTGTGCAGAGAAAGAGGAAGAAAACCAGG
  AGAATTTGGATTGGGTGCCTCTCACCACACTGTCACACTGCAAGTCACTGAGGACAATGACAGCAATCAG
  TCGCTACATGGAGCTGACCATCGAGCCTGTGCAGCAGGCTGCAGTGCCACCAGGCTGCCAGGTGACGGGC
  AGACGAGCGCCGGAGATGCGTCCCTGCAGGACCCCCCGTCGTACCCTCCCGTTCAGGTCATCCGGGCCCG
  GGTGTCTTCCGGCAGCTCCTCTGAGGTCTCCTCCATCAACTCCGACCTGGAGTGGGACCCTGAAGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202358 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGEAPSPAPALWDWDYLDRCFARNRVCISFGLWICASSCWIAAHALLLYLRCAQKPRQDQSALCAACCLL
  TSLCDTVGALLARQLTIQVFTGAYLAAIDLVNFMFILFPVCGSKFKSNSDREARERKRRRQLRASVFALA
  LPLSLGPCWALWVAVPKASATIRGPQRRLLASLLQENTEILGYLLGSVAAFGSWASRIPPLSRICRGKTF
  PSIHLWTRLLSALAGLLYASAIVAHDRHPEYLLRATPWFLTSLGRAALDLAIIFLSCVMKSKMRQALGFA
  KEARESPDTQALLTCAEKEEENQENLDWVPLTTLSHCKSLRTMTAISRYMELTIEPVQQAAVPPGCQVTG
  RRAPEMRPCRTPRRTLPFRSSGPGCLPAAPLRSPPSTPTWSGTLKM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001011655
• ORF Size: 477 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 2362 bp
• RefSeq ORF: 1287 bp
• Locus ID: 93109
• UniProt ID: Q2T9K0
• Cytogenetics: 3q29
• Protein Families: Transmembrane
• MW: 43.8 kDa