GGH (NM_003878) Human Tagged ORF Clone

CAT#: RC202265

GGH (Myc-DDK-tagged)-Human gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase) (GGH)

Product Images

Circular map for RC202265

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  Western blot validation of overexpression lysate (Cat# LY418375) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202265 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GGH protein (Cat# TP302265). The protein was produced from HEK293T cells transfected with GGH cDNA clone (Cat# RC202265) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GGH
• Synonyms: GATD10; GH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202265 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGTCCGGGCTGCCTGCTGTGCGTGCTGGGCCTGCTACTCTGCGGGGCGGCGAGCCTCGAGCTGT
  CTAGACCCCACGGCGACACCGCCAAGAAGCCCATCATCGGAATATTAATGCAAAAATGCCGTAATAAAGT
  CATGAAAAACTATGGAAGATACTATATTGCTGCGTCCTATGTAAAGTACTTGGAGTCTGCAGGTGCGAGA
  GTTGTACCAGTAAGGCTGGATCTTACAGAGAAAGACTATGAAATACTTTTCAAATCTATTAATGGAATCC
  TTTTCCCTGGAGGAAGTGTTGACCTCAGACGCTCAGATTATGCTAAAGTGGCCAAAATATTTTATAACTT
  GTCCATACAGAGTTTTGATGATGGAGACTATTTTCCTGTGTGGGGCACATGCCTTGGATTTGAAGAGCTT
  TCACTGCTGATTAGTGGAGAGTGCTTATTAACTGCCACAGATACTGTTGACGTGGCAATGCCGCTGAACT
  TCACTGGAGGTCAATTGCACAGCAGAATGTTCCAGAATTTTCCTACTGAGTTGTTGCTGTCATTAGCAGT
  AGAACCTCTGACTGCCAATTTCCATAAGTGGAGCCTCTCCGTGAAGAATTTTACAATGAATGAAAAGTTA
  AAGAAGTTTTTCAATGTCTTAACTACAAATACAGATGGCAAGATTGAGTTTATTTCAACAATGGAAGGAT
  ATAAGTATCCAGTATATGGTGTCCAGTGGCATCCAGAGAAAGCACCTTATGAGTGGAAGAATTTGGATGG
  CATTTCCCATGCACCTAATGCTGTGAAAACCGCATTTTATTTAGCAGAGTTTTTTGTTAATGAAGCTCGG
  AAAAACAACCATCATTTTAAATCTGAATCTGAAGAGGAGAAAGCATTGATTTATCAGTTCAGTCCAATTT
  ATACTGGAAATATTTCTTCATTTCAGCAATGTTACATATTTGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202265 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASPGCLLCVLGLLLCGAASLELSRPHGDTAKKPIIGILMQKCRNKVMKNYGRYYIAASYVKYLESAGAR
  VVPVRLDLTEKDYEILFKSINGILFPGGSVDLRRSDYAKVAKIFYNLSIQSFDDGDYFPVWGTCLGFEEL
  SLLISGECLLTATDTVDVAMPLNFTGGQLHSRMFQNFPTELLLSLAVEPLTANFHKWSLSVKNFTMNEKL
  KKFFNVLTTNTDGKIEFISTMEGYKYPVYGVQWHPEKAPYEWKNLDGISHAPNAVKTAFYLAEFFVNEAR
  KNNHHFKSESEEEKALIYQFSPIYTGNISSFQQCYIFD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003878
• ORF Size: 954 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003878.3
• RefSeq Size: 1505 bp
• RefSeq ORF: 957 bp
• Locus ID: 8836
• UniProt ID: Q92820
• Cytogenetics: 8q12.3
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Folate biosynthesis
• MW: 36 kDa
• Gene Summary: This gene catalyzes the hydrolysis of folylpoly-gamma-glutamates and antifolylpoly-gamma-glutamates by the removal of gamma-linked polyglutamates and glutamate. [provided by RefSeq, Jul 2008]