SRPR alpha (SRPRA) (NM_003139) Human Tagged ORF Clone

CAT#: RC202241

SRPR (Myc-DDK-tagged)-Human signal recognition particle receptor (docking protein) (SRPR), transcript variant 1

Product Images

Circular map for RC202241

• 

  Western blot validation of overexpression lysate (Cat# LY418871) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202241 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SRPR alpha
• Synonyms: DP; Sralpha; SRPR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202241 representing NM_003139
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCGACTTCTTCACCATTTTCTCCAAGGGCGGGCTTGTGCTCTGGTGCTTCCAGGGCGTTAGCGACT
  CATGCACCGGACCCGTTAACGCGTTGATTCGTTCCGTGCTGCTGCAGGAACGGGGAGGTAACAACTCCTT
  CACCCATGAGGCACTCACACTCAAGTATAAACTGGACAACCAGTTTGAGCTGGTGTTTGTGGTTGGTTTT
  CAGAAGATCCTGACACTGACATATGTAGACAAATTGATAGATGACGTGCATCGGCTGTTTCGGGACAAGT
  ACCGCACAGAGATCCAACAGCAAAGTGCTTTAAGTTTATTAAATGGCACTTTTGATTTCCAAAATGACTT
  CCTGCGGCTCCTTCGTGAAGCAGAGGAGAGCAGTAAGATCCGTGCTCCCACTACCATGAAGAAATTTGAA
  GATTCTGAAAAGGCCAAGAAACCTGTGAGGTCCATGATTGAGACACGGGGGGAAAAGCCCAAGGAAAAAG
  CAAAGAATAGCAAAAAAAAGGGGGCCAAGAAGGAAGGTTCTGATGGTCCTTTGGCTACCAGCAAACCAGT
  CCCTGCAGAAAAGTCAGGTCTTCCAGTGGGTCCTGAGAACGGAGTAGAACTTTCCAAAGAGGAGCTGATC
  CGCAGGAAGCGCGAGGAGTTCATTCAGAAGCATGGGAGGGGTATGGAGAAGTCCAACAAGTCCACGAAGT
  CAGATGCTCCAAAGGAGAAGGGCAAAAAAGCACCCCGGGTGTGGGAACTGGGTGGCTGTGCTAACAAAGA
  AGTGTTGGATTACAGTACTCCCACCACCAATGGAACCCCTGAGGCTGCCTTGTCTGAGGACATCAACCTG
  ATTCGAGGGACTGGGTCTGGGGGGCAGCTTCAGGATCTGGACTGCAGCAGCTCTGATGACGAAGGGGCTG
  CTCAAAACTCTACCAAACCTAGTGCGACCAAGGGAACACTGGGTGGCATGTTTGGTATGCTGAAGGGCCT
  TGTGGGTTCAAAGAGCTTGAGTCGTGAAGACATGGAATCTGTGCTGGACAAGATGCGTGATCATCTCATT
  GCTAAGAACGTGGCTGCAGACATTGCCGTCCAGCTCTGTGAATCTGTTGCCAACAAGTTGGAAGGGAAGG
  TGATGGGGACGTTCAGCACGGTGACTTCCACAGTAAAGCAAGCCCTACAGGAGTCCCTGGTGCAGATTCT
  GCAGCCACAGCGTCGTGTAGACATGCTCCGGGACATCATGGATGCCCAGCGTCGCCAGCGCCCTTATGTC
  GTCACCTTCTGCGGCGTTAATGGAGTGGGGAAATCTACTAATCTTGCCAAGATTTCCTTCTGGTTGTTAG
  AGAATGGCTTCAGTGTCCTCATTGCTGCCTGTGATACATTTCGTGCTGGGGCCGTGGAGCAGCTGCGTAC
  ACACACCCGGCGTTTGAGTGCCCTACACCCTCCAGAGAAGCATGGTGGCCGCACCATGGTGCAGTTGTTT
  GAAAAGGGCTATGGCAAGGATGCTGCTGGCATTGCCATGGAAGCCATTGCTTTTGCACGTAACCAAGGCT
  TTGACGTGGTGCTGGTGGACACGGCAGGCCGCATGCAAGACAATGCCCCTCTGATGACTGCCCTGGCCAA
  ACTCATTACTGTCAATACACCTGATTTGGTGCTGTTTGTAGGAGAAGCCTTAGTAGGCAATGAAGCCGTG
  GACCAGCTGGTCAAGTTCAACAGAGCCTTGGCTGACCATTCTATGGCTCAGACACCTCGGCTCATTGATG
  GCATTGTTCTTACCAAATTTGATACCATTGATGACAAGGTGGGAGCTGCTATTTCTATGACGTACATCAC
  AAGCAAACCCATCGTCTTTGTGGGCACCGGCCAGACCTACTGTGACCTACGCAGCCTCAATGCCAAGGCT
  GTGGTGGCTGCCCTCATGAAGGCT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202241 representing NM_003139
  Red=Cloning site Green=Tags(s)
  
  MLDFFTIFSKGGLVLWCFQGVSDSCTGPVNALIRSVLLQERGGNNSFTHEALTLKYKLDNQFELVFVVGF
  QKILTLTYVDKLIDDVHRLFRDKYRTEIQQQSALSLLNGTFDFQNDFLRLLREAEESSKIRAPTTMKKFE
  DSEKAKKPVRSMIETRGEKPKEKAKNSKKKGAKKEGSDGPLATSKPVPAEKSGLPVGPENGVELSKEELI
  RRKREEFIQKHGRGMEKSNKSTKSDAPKEKGKKAPRVWELGGCANKEVLDYSTPTTNGTPEAALSEDINL
  IRGTGSGGQLQDLDCSSSDDEGAAQNSTKPSATKGTLGGMFGMLKGLVGSKSLSREDMESVLDKMRDHLI
  AKNVAADIAVQLCESVANKLEGKVMGTFSTVTSTVKQALQESLVQILQPQRRVDMLRDIMDAQRRQRPYV
  VTFCGVNGVGKSTNLAKISFWLLENGFSVLIAACDTFRAGAVEQLRTHTRRLSALHPPEKHGGRTMVQLF
  EKGYGKDAAGIAMEAIAFARNQGFDVVLVDTAGRMQDNAPLMTALAKLITVNTPDLVLFVGEALVGNEAV
  DQLVKFNRALADHSMAQTPRLIDGIVLTKFDTIDDKVGAAISMTYITSKPIVFVGTGQTYCDLRSLNAKA
  VVAALMKA
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003139
• ORF Size: 1914 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003139.4
• RefSeq Size: 2958 bp
• RefSeq ORF: 1917 bp
• Locus ID: 6734
• UniProt ID: P08240
• Cytogenetics: 11q24.2
• Domains: SRP54, AAA, SRP-alpha_N
• Protein Families: Druggable Genome
• Protein Pathways: Protein export
• MW: 69.6 kDa
• Gene Summary: The gene encodes a subunit of the endoplasmic reticulum signal recognition particle receptor that, in conjunction with the signal recognition particle, is involved in the targeting and translocation of signal sequence tagged secretory and membrane proteins across the endoplasmic reticulum. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]