Calpastatin (CAST) (NM_001042444) Human Tagged ORF Clone

CAT#: RC202168

CAST (Myc-DDK-tagged)-Human calpastatin (CAST), transcript variant 10

Product Images

Circular map for RC202168

• 

  Western blot validation of overexpression lysate (Cat# LY420908) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202168 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CAST protein (Cat# TP302168). The protein was produced from HEK293T cells transfected with CAST cDNA clone (Cat# RC202168) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Calpastatin
• Synonyms: BS-17; PLACK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202168 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCCCACAGAAACCAAGGCTGTAAAAACAGAACCTGAGAAGAAGTCACAGTCAACCAAGCCAAAAA
  GCCTACCCAAGCAGGCATCAGATACAGGAAGTAACGATGCTCACAATAAAAAAGCAGTTTCCAGATCAGC
  TGAACAGCAGCCATCAGAGAAATCAACAGAACCAAAGACTAAACCACAAGACATGATTTCTGCTGGTGGA
  GAGAGTGTTGCTGGTATCACTGCAATATCTGGCAAGCCGGGTGACAAGAAAAAAGAAAAGAAATCATTAA
  CCCCAGCTGTGCCAGTTGAATCTAAACCGGATAAACCATCGGGAAAGTCAGGCATGGATGCTGCTTTGGA
  TGACTTAATAGATACTTTAGGAGGACCTGAAGAAACTGAAGAAGAAAATACAACGTATACTGGACCAGAA
  GTTTCAGATCCAATGAGTTCCACCTACATAGAGGAATTGGGTAAAAGAGAAGTCACAATTCCTCCAAAAT
  ATAGGGAACTATTGGCTAAAAAGGAAGGGATCACAGGGCCTCCTGCAGACTCTTCGAAACCCATAGGGCC
  AGATGATGCTATAGACGCCTTGTCATCTGACTTCACCTGTGGGTCGCCTACAGCTGCTGGAAAGAAAACT
  GAAAAAGAGGAATCTACAGAAGTTTTAAAAGCTCAGTCAGCAGGGACAGTCAGAAGTGCTGCTCCACCCC
  AAGAGAAGAAAAGAAAGGTGGAGAAGGATACAATGAGTGATCAAGCACTCGAGGCTCTGTCGGCTTCACT
  GGGCACCCGGCAAGCAGAACCTGAGCTCGACCTCCGCTCAATTAAGGAAGTCGATGAGGCAAAAGCTAAA
  GAAGAAAAACTAGAGAAGTGTGGTGAGGATGATGAAACAATCCCATCTGAGTACAGATTAAAACCAGCCA
  CGGATAAAGATGGAAAACCACTATTGCCAGAGCCTGAAGAAAAACCCAAGCCTCGGAGTGAATCAGAACT
  CATTGATGAACTTTCAGAAGATTTTGACCGGTCTGAATGTAAAGAGAAACCATCTAAGCCAACTGAAAAG
  ACAGAAGAATCTAAGGCCGCTGCTCCAGCTCCTGTGTCGGAGGCTGTGTGTCGGACCTCCATGTGTAGTA
  TACAGTCAGCACCCCCTGAGCCGGCTACCTTGAAGGGCACAGTGCCAGATGATGCTGTAGAAGCCTTGGC
  TGATAGCCTGGGGAAAAAGGAAGCAGATCCAGAAGATGGAAAACCTGTGATGGATAAAGTCAAGGAGAAG
  GCCAAAGAAGAAGACCGTGAAAAGCTTGGTGAAAAAGAAGAAACAATTCCTCCTGATTATAGATTAGAAG
  AGGTCAAGGATAAAGATGGAAAGCCACTCCTGCCAAAAGAGTCTAAGGAACAGCTTCCACCCATGAGTGA
  AGACTTCCTTCTGGATGCTTTGTCTGAGGACTTCTCTGGTCCACAAAATGCTTCATCTCTTAAATTTGAA
  GATGCTAAACTTGCTGCTGCCATCTCTGAAGTGGTTTCCCAAACCCCAGCTTCAACGACCCAAGCTGGAG
  CCCCACCCCGTGATACCTCGCAGAGTGACAAAGACCTCGATGATGCCTTGGATAAACTCTCTGACAGTCT
  AGGACAAAGGCAGCCTGACCCAGATGAGAACAAACCAATGGAAGATAAAGTAAAGGAAAAAGCTAAAGCT
  GAACATAGAGACAAGCTTGGAGAAAGAGATGACACTATCCCACCTGAATACAGACATCTCCTGGATGATA
  ATGGACAGGACAAACCAGTGAAGCCACCTACAAAGAAATCAGAGGATTCAAAGAAACCTGCAGATGACCA
  AGACCCCATTGATGCTCTCTCAGGAGATCTGGACAGCTGTCCCTCCACTACAGAAACCTCACAGAACACA
  GCAAAGGATAAGTGCAAGAAGGCTGCTTCCAGCTCCAAAGCACCTAAGAATGGAGGTAAAGCGAAGGATT
  CAGCAAAGACAACAGAGGAAACTTCCAAGCCAAAAGATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202168 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNPTETKAVKTEPEKKSQSTKPKSLPKQASDTGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGG
  ESVAGITAISGKPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEEENTTYTGPE
  VSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADSSKPIGPDDAIDALSSDFTCGSPTAAGKKT
  EKEESTEVLKAQSAGTVRSAAPPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAK
  EEKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDELSEDFDRSECKEKPSKPTEK
  TEESKAAAPAPVSEAVCRTSMCSIQSAPPEPATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEK
  AKEEDREKLGEKEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGPQNASSLKFE
  DAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALDKLSDSLGQRQPDPDENKPMEDKVKEKAKA
  EHRDKLGERDDTIPPEYRHLLDDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNT
  AKDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001042444
• ORF Size: 2001 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042444.2
• RefSeq Size: 4366 bp
• RefSeq ORF: 2004 bp
• Locus ID: 831
• UniProt ID: P20810
• Cytogenetics: 5q15
• MW: 71.9 kDa
• Gene Summary: The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]