ORC1 (NM_004153) Human Tagged ORF Clone

CAT#: RC202058

ORC1 (Myc-DDK-tagged)-Human origin recognition complex, subunit 1 (ORC1), transcript variant 1

Product Images

Circular map for RC202058

• 

  Western blot validation of overexpression lysate (Cat# LY418180) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202058 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ORC1
• Synonyms: HSORC1; ORC1L; PARC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202058 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACACTACCCCACAAGGCTGACTACCAGAAAAACTTATTCATGGGTTGGCAGGCCCTTGTTGGATC
  GAAAACTGCACTACCAAACCTATAGAGAAATGTGTGTGAAAACAGAAGGTTGTTCCACCGAGATTCACAT
  CCAGATTGGACAGTTTGTGTTGATTGAAGGGGATGATGATGAAAACCCGTATGTTGCTAAATTGCTTGAG
  TTGTTCGAAGATGACTCTGATCCTCCTCCTAAGAAACGTGCTCGAGTACAGTGGTTTGTCCGATTCTGTG
  AAGTCCCTGCCTGTAAACGGCATTTGTTGGGCCGGAAGCCTGGTGCACAGGAAATATTCTGGTATGATTA
  CCCGGCCTGTGACAGCAACATTAATGCGGAGACCATCATTGGCCTTGTTCGGGTGATACCTTTAGCCCCA
  AAGGATGTGGTACCGACGAATCTGAAAAATGAGAAGACACTCTTTGTGAAACTATCCTGGAATGAGAAGA
  AATTCAGGCCACTTTCCTCAGAACTATTTGCGGAGTTGAATAAACCACAAGAGAGTGCAGCCAAGTGCCA
  GAAACCCGTGAGAGCCAAGAGTAAGAGTGCAGAGAGCCCTTCTTGGACCCCAGCAGAACATGTGGCCAAA
  AGGATTGAATCAAGGCACTCCGCCTCCAAATCTCGCCAAACTCCTACCCATCCTCTTACCCCAAGAGCCA
  GAAAGAGGCTGGAGCTTGGCAACTTAGGTAACCCTCAGATGTCCCAGCAGACTTCATGTGCCTCCTTGGA
  TTCTCCAGGAAGAATAAAACGGAAAGTGGCCTTCTCGGAGATCACCTCACCTTCTAAGAGATCTCAGCCT
  GATAAACTTCAAACCTTGTCTCCAGCTCTGAAAGCCCCAGAGAAAACCAGAGAGACTGGACTCTCTTATA
  CTGAGGATGACAAGAAGGCTTCACCTGAACATCGCATAATCCTGAGAACCCGAATTGCAGCTTCGAAAAC
  CATAGACATTAGAGAGGAGAGAACACTTACCCCTATCAGTGGGGGACAGAGATCTTCAGTGGTGCCATCC
  GTGATTCTGAAACCAGAAAACATCAAAAAGAGGGATGCAAAAGAAGCAAAAGCCCAGAATGAAGCGACCT
  CTACTCCCCATCGTATCCGCAGAAAGAGTTCTGTCTTGACTATGAATCGGATTAGGCAGCAGCTTCGGTT
  TCTAGGTAATAGTAAAAGTGACCAAGAAGAGAAAGAGATTCTGCCAGCAGCAGAGATTTCAGACTCTAGC
  AGTGACGAAGAAGAGGCTTCCACACCGCCCCTTCCAAGGAGAGCACCCAGAACTGTGTCCAGGAACCTGC
  GATCTTCCTTGAAGTCATCCTTACATACCCTCACGAAGGTGCCAAAGAAGAGTCTCAAGCCTAGAACGCC
  ACGTTGTGCCGCTCCTCAGATCCGTAGTCGAAGCCTGGCTGCCCAGGAGCCAGCCAGTGTGCTGGAGGAA
  GCCCGACTGAGGCTGCATGTTTCTGCTGTACCTGAGTCTCTTCCCTGTCGGGAACAGGAATTCCAAGACA
  TCTACAATTTTGTGGAAAGCAAACTCCTTGACCATACCGGAGGGTGCATGTACATCTCCGGTGTCCCTGG
  GACAGGGAAGACTGCCACTGTTCATGAAGTGATACGCTGCCTGCAGCAGGCAGCCCAAGCCAATGATGTT
  CCTCCCTTTCAATACATTGAGGTCAATGGCATGAAGCTGACGGAGCCCCACCAAGTCTATGTGCAAATCT
  TGCAGAAGCTAACAGGCCAAAAAGCAACAGCCAACCATGCGGCAGAACTGCTGGCAAAGCAATTCTGCAC
  CCGAGGGTCACCTCAGGAAACCACCGTCCTGCTTGTGGATGAGCTCGACCTTCTGTGGACTCACAAACAA
  GACATAATGTACAATCTCTTTGACTGGCCCACTCATAAGGAGGCCCGGCTTGTGGTCCTGGCAATTGCCA
  ACACAATGGACCTGCCAGAGCGAATCATGATGAACCGGGTGTCCAGCCGACTGGGTCTTACCAGGATGTG
  CTTCCAGCCCTATACATATAGCCAGCTGCAGCAGATCCTAAGGTCCCGGCTCAAGCATCTAAAGGCCTTT
  GAAGATGATGCCATCCAGCTGGTAGCCAGGAAGGTAGCAGCACTGTCTGGAGATGCACGACGGTGCCTGG
  ACATCTGCAGGCGTGCCACAGAGATCTGTGAGTTCTCCCAGCAGAAGCCTGACTCCCCTGGCCTGGTCAC
  CATAGCCCACTCAATGGAAGCTGTGGATGAGATGTTTTCATCATCATACATCACGGCCATCAAAAATTCC
  TCTGTTCTGGAACAGAGCTTCCTGAGAGCCATCCTCGCAGAGTTCCGTCGATCAGGACTGGAGGAAGCCA
  CGTTTCAACAGATATATAGTCAACATGTGGCACTGTGCAGAATGGAGGGACTGCCGTACCCCACCATGTC
  AGAGACCATGGCCGTGTGTTCTCACCTGGGCTCCTGTCGCCTCCTGCTTGTGGAGCCCAGCAGGAACGAT
  CTGCTCCTTCGGGTGCGGCTCAACGTCAGCCAGGATGATGTGCTGTATGCGCTGAAAGACGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202058 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAHYPTRLTTRKTYSWVGRPLLDRKLHYQTYREMCVKTEGCSTEIHIQIGQFVLIEGDDDENPYVAKLLE
  LFEDDSDPPPKKRARVQWFVRFCEVPACKRHLLGRKPGAQEIFWYDYPACDSNINAETIIGLVRVIPLAP
  KDVVPTNLKNEKTLFVKLSWNEKKFRPLSSELFAELNKPQESAAKCQKPVRAKSKSAESPSWTPAEHVAK
  RIESRHSASKSRQTPTHPLTPRARKRLELGNLGNPQMSQQTSCASLDSPGRIKRKVAFSEITSPSKRSQP
  DKLQTLSPALKAPEKTRETGLSYTEDDKKASPEHRIILRTRIAASKTIDIREERTLTPISGGQRSSVVPS
  VILKPENIKKRDAKEAKAQNEATSTPHRIRRKSSVLTMNRIRQQLRFLGNSKSDQEEKEILPAAEISDSS
  SDEEEASTPPLPRRAPRTVSRNLRSSLKSSLHTLTKVPKKSLKPRTPRCAAPQIRSRSLAAQEPASVLEE
  ARLRLHVSAVPESLPCREQEFQDIYNFVESKLLDHTGGCMYISGVPGTGKTATVHEVIRCLQQAAQANDV
  PPFQYIEVNGMKLTEPHQVYVQILQKLTGQKATANHAAELLAKQFCTRGSPQETTVLLVDELDLLWTHKQ
  DIMYNLFDWPTHKEARLVVLAIANTMDLPERIMMNRVSSRLGLTRMCFQPYTYSQLQQILRSRLKHLKAF
  EDDAIQLVARKVAALSGDARRCLDICRRATEICEFSQQKPDSPGLVTIAHSMEAVDEMFSSSYITAIKNS
  SVLEQSFLRAILAEFRRSGLEEATFQQIYSQHVALCRMEGLPYPTMSETMAVCSHLGSCRLLLVEPSRND
  LLLRVRLNVSQDDVLYALKDE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004153
• ORF Size: 2583 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004153.4
• RefSeq Size: 3192 bp
• RefSeq ORF: 2586 bp
• Locus ID: 4998
• UniProt ID: Q13415
• Cytogenetics: 1p32.3
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Cell cycle
• MW: 97.3 kDa
• Gene Summary: The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in control of transcription silencing. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]