Glycogenin 2 (GYG2) (NM_001079855) Human Tagged ORF Clone

CAT#: RC202043

GYG2 (Myc-DDK-tagged)-Human glycogenin 2 (GYG2), transcript variant 1

Product Images

Circular map for RC202043

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  Western blot validation of overexpression lysate (Cat# LY421559) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202043 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GYG2 protein (Cat# TP302043). The protein was produced from HEK293T cells transfected with GYG2 cDNA clone (Cat# RC202043) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Glycogenin 2
• Synonyms: GN-2; GN2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202043 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGTGACTGATCAGGCTTTTGTCACACTAGCCACCAATGACATCTACTGCCAGGGCGCCCTGGTCC
  TGGGGCAGTCACTGAGGAGACACAGGCTGACGAGGAAGCTGGTGGTGTTGATCACTCCTCAGGTGTCCAG
  CCTGCTCAGGGTCATCCTCTCGAAGGTGTTCGATGAAGTCATTGAAGTGAATCTAATCGATAGTGCCGAC
  TACATCCACCTGGCCTTTCTGAAGAGACCTGAGCTCGGGCTCACCCTCACCAAGCTTCACTGTTGGACTC
  TCACTCACTACAGCAAGTGTGTCTTCCTGGATGCAGACACTCTGGTGCTGTCCAATGTCGATGAGCTGTT
  TGACAGGGGAGAGTTTTCTGCGGCCCCGGACCCCGGATGGCCGGATTGCTTCAATAGCGGGGTGTTTGTC
  TTCCAGCCTTCTCTCCACACGCATAAACTCCTGCTACAGCACGCCATGGAACACGGCAGCTTTGACGGGG
  CAGACCAAGGCTTACTGAATAGTTTCTTCAGGAACTGGTCGACCACAGACATCCACAAGCACCTGCCGTT
  CATCTATAACTTGAGTAGTAACACGATGTACACTTACAGCCCTGCCTTCAAGCAATTCGGTTCCAGTGCA
  AAGGTCGTCCACTTTTTGGGGTCCATGAAACCTTGGAACTACAAGTACAATCCACAGAGTGGCTCGGTGT
  TGGAGCAAGGCTCAGTGTCCAGCAGCCAGCACCAGGCGGCATTCCTTCATCTCTGGTGGACGGTCTACCA
  GAACAACGTGCTGCCCCTTTATAAAAGCGTCCAAGCGGGGGAAGCACGCGCGTCTCCTGGTCACACACTT
  TGCCGCAGTGATGTGGGGGGGCCGTGTGCGGATTCAGCCTCTGGTGTTGGAGAGCCGTGTGAAAATTCAA
  CACCCAGTGCGGGCGTGCCGTGTGCAAATTCACCACTGGGTTCTAACCAGCCTGCTCAGGGCCTTCCGGA
  GCCGACCCAGATAGTGGATGAGACCCTGTCCCTACCTGAAGGACGCCGTTCAGAAGATATGATAGCTTGT
  CCTGAAACTGAGACTCCTGCCGTGATAACGTGTGACCCACTGTCCCAGCCTTCCCCTCAGCCTGCAGACT
  TCACAGAGACTGAAACCATCTTGCAGCCAGCAAATAAAGTCGAAAGTGTCTCATCCGAGGAAACCTTCGA
  ACCAAGCCAGGAACTCCCTGCTGAGGCTCTCAGGGACCCCAGTCTGCAGGATGCACTGGAGGTCGACCTG
  GCCGTCTCTGTTTCCCAGATCTCCATCGAAGAGAAGGTGAAGGAATTGAGCCCCGAGGAAGAGAGGAGGA
  AGTGGGAGGAAGGCCGTATCGACTACATGGGGAAGGACGCGTTTGCTCGCATCCAGGAGAAGCTGGACCG
  GTTCCTGCAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202043 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSVTDQAFVTLATNDIYCQGALVLGQSLRRHRLTRKLVVLITPQVSSLLRVILSKVFDEVIEVNLIDSAD
  YIHLAFLKRPELGLTLTKLHCWTLTHYSKCVFLDADTLVLSNVDELFDRGEFSAAPDPGWPDCFNSGVFV
  FQPSLHTHKLLLQHAMEHGSFDGADQGLLNSFFRNWSTTDIHKHLPFIYNLSSNTMYTYSPAFKQFGSSA
  KVVHFLGSMKPWNYKYNPQSGSVLEQGSVSSSQHQAAFLHLWWTVYQNNVLPLYKSVQAGEARASPGHTL
  CRSDVGGPCADSASGVGEPCENSTPSAGVPCANSPLGSNQPAQGLPEPTQIVDETLSLPEGRRSEDMIAC
  PETETPAVITCDPLSQPSPQPADFTETETILQPANKVESVSSEETFEPSQELPAEALRDPSLQDALEVDL
  AVSVSQISIEEKVKELSPEEERRKWEEGRIDYMGKDAFARIQEKLDRFLQ
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001079855
• ORF Size: 1410 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001079855.1, NP_001073324.1
• RefSeq Size: 3318 bp
• RefSeq ORF: 1413 bp
• Locus ID: 8908
• UniProt ID: O15488
• Cytogenetics: Xp22.33
• MW: 52 kDa
• Gene Summary: This gene encodes a member of the the glycogenin family. Glycogenin is a self-glucosylating protein involved in the initiation reactions of glycogen biosynthesis. A gene on chromosome 3 encodes the muscle glycogenin and this X-linked gene encodes the glycogenin mainly present in liver; both are involved in blood glucose homeostasis. This gene has a short version on chromosome Y, which is 3' truncated and can not make a functional protein. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, May 2010]