SCO1 (NM_004589) Human Tagged ORF Clone

CAT#: RC202026

SCO1 (Myc-DDK-tagged)-Human SCO cytochrome oxidase deficient homolog 1 (yeast) (SCO1), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RC202026

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  Western blot validation of overexpression lysate (Cat# LY417885) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202026 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SCO1 protein (Cat# TP302026). The protein was produced from HEK293T cells transfected with SCO1 cDNA clone (Cat# RC202026) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SCO1
• Synonyms: MC4DN4; SCOD1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202026 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGATGCTGGTCCTAGTACCCGGACGAGTTATGCGGCCTCTGGGTGGCCAACTTTGGCGCTTCTTGC
  CTCGCGGACTCGAGTTTTGGGGCCCAGCCGAGGGGACTGCGAGAGTCTTGCTGAGGCAGTTCTGCGCGCG
  GCAAGCGGAGGCGTGGCGTGCCTCGGGGCGCCCTGGCTATTGCCTGGGAACCCGGCCCCTCAGCACTGCG
  AGGCCGCCACCCCCGTGGTCGCAGAAGGGCCCCGGAGACTCCACGCGCCCCTCGAAGCCCGGGCCTGTTT
  CCTGGAAGTCTTTAGCAATCACATTTGCTATTGGAGGAGCTTTACTGGCTGGAATGAAGCACGTCAAGAA
  AGAAAAGGCAGAGAAGTTAGAGAAGGAACGGCAGCGACACATCGGCAAGCCTTTACTTGGGGGACCGTTT
  TCCCTCACAACTCATACTGGGGAGCGTAAAACTGACAAGGACTACTTGGGTCAGTGGTTATTGATTTATT
  TTGGCTTCACTCATTGCCCTGATGTCTGTCCAGAAGAACTAGAAAAGATGATTCAAGTCGTGGATGAAAT
  AGATAGCATTACAACTCTGCCAGATCTAACTCCACTTTTCATCAGCATTGACCCAGAGAGGGACACAAAA
  GAAGCCATCGCAAATTATGTGAAAGAATTTTCTCCCAAACTGGTTGGCTTGACTGGCACGAGAGAAGAGG
  TCGATCAAGTGGCCAGAGCATACAGAGTGTATTACAGCCCTGGCCCCAAGGACGAAGATGAAGACTACAT
  AGTGGATCACACAATAATAATGTACTTGATTGGACCAGATGGTGAGTTTCTAGATTATTTTGGCCAGAAC
  AAGAGGAAGGGAGAAATAGCTGCTTCAATTGCCACACACATGAGGCCATACAGAAAAAAGAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202026 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAMLVLVPGRVMRPLGGQLWRFLPRGLEFWGPAEGTARVLLRQFCARQAEAWRASGRPGYCLGTRPLSTA
  RPPPPWSQKGPGDSTRPSKPGPVSWKSLAITFAIGGALLAGMKHVKKEKAEKLEKERQRHIGKPLLGGPF
  SLTTHTGERKTDKDYLGQWLLIYFGFTHCPDVCPEELEKMIQVVDEIDSITTLPDLTPLFISIDPERDTK
  EAIANYVKEFSPKLVGLTGTREEVDQVARAYRVYYSPGPKDEDEDYIVDHTIIMYLIGPDGEFLDYFGQN
  KRKGEIAASIATHMRPYRKKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004589
• ORF Size: 903 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004589.4
• RefSeq Size: 1768 bp
• RefSeq ORF: 906 bp
• Locus ID: 6341
• UniProt ID: O75880
• Cytogenetics: 17p13.1
• Domains: SCO1-SenC
• MW: 33.8 kDa
• Gene Summary: Mammalian cytochrome c oxidase (COX) catalyzes the transfer of reducing equivalents from cytochrome c to molecular oxygen and pumps protons across the inner mitochondrial membrane. In yeast, 2 related COX assembly genes, SCO1 and SCO2 (synthesis of cytochrome c oxidase), enable subunits 1 and 2 to be incorporated into the holoprotein. This gene is the human homolog to the yeast SCO1 gene. [provided by RefSeq, Jul 2008]