DDX23 (NM_004818) Human Tagged ORF Clone

CAT#: RC201758

DDX23 (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 23 (DDX23)

Product Images

Circular map for RC201758

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  Western blot validation of overexpression lysate (Cat# LY401514) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201758 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified DDX23 protein (Cat# TP301758). The protein was produced from HEK293T cells transfected with DDX23 cDNA clone (Cat# RC201758) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DDX23
• Synonyms: prp28; PRPF28; SNRNP100; U5-100K; U5-100KD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201758 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGAGAGCTGGCTGACAAAAAGGACCGTGATGCATCACCTTCCAAGGAGGAAAGGAAGCGATCAC
  GGACTCCTGACAGAGAGCGGGATAGAGACCGGGACCGGAAGTCTTCCCCATCTAAAGATAGAAAGCGGCA
  TCGTTCAAGGGATAGACGTCGAGGAGGCAGCCGTTCTCGCTCTCGTTCCCGTTCCAAATCTGCAGAAAGA
  GAACGACGGCACAAAGAACGAGAACGAGATAAGGAGCGGGATCGGAATAAGAAGGACCGAGATCGAGACA
  AGGATGGGCACAGACGGGACAAGGACCGTAAACGATCCAGCTTATCTCCTGGTCGAGGAAAAGACTTTAA
  ATCTCGGAAGGACAGAGACTCTAAGAAGGATGAAGAGGATGAACATGGTGATAAGAAGCCTAAGGCCCAG
  CCATTATCCCTGGAGGAGCTTCTGGCCAAGAAAAAGGCTGAGGAAGAAGCTGAGGCTAAGCCCAAGTTCC
  TCTCTAAAGCAGAACGAGAGGCTGAAGCTCTAAAGCGACGGCAGCAGGAGGTGGAAGAGCGGCAGAGGAT
  GCTTGAAGAAGAGAGGAAGAAAAGGAAACAGTTCCAAGACTTGGGCAGGAAGATGTTGGAAGATCCTCAG
  GAACGGGAACGTCGGGAACGCAGGGAGAGGATGGAACGGGAGACCAATGGAAATGAGGATGAGGAAGGGC
  GGCAGAAGATCCGGGAAGAGAAGGATAAGAGCAAGGAACTGCATGCCATTAAGGAGCGTTACCTGGGTGG
  CATCAAAAAGCGGCGCCGAACGAGACATCTCAATGACCGGAAATTTGTTTTTGAGTGGGATGCATCTGAG
  GACACATCCATTGACTACAACCCCCTGTACAAAGAACGGCACCAGGTGCAGTTGTTAGGGCGAGGCTTCA
  TTGCAGGCATTGACCTCAAGCAGCAGAAGCGAGAGCAGTCACGTTTCTATGGAGACCTAATGGAGAAGAG
  GCGAACCCTGGAAGAAAAGGAGCAGGAGGAGGCAAGACTCCGCAAACTTCGTAAGAAGGAAGCCAAGCAG
  CGCTGGGATGATCGTCATTGGTCTCAGAAAAAGTTAGATGAGATGACGGACAGGGACTGGCGGATCTTCC
  GTGAGGACTACAGCATCACCACCAAAGGTGGCAAGATCCCCAATCCCATCCGATCCTGGAAAGACTCTTC
  TCTGCCCCCACACATCTTGGAGGTCATTGATAAGTGTGGCTACAAGGAACCAACACCTATACAGCGTCAG
  GCAATTCCCATTGGGCTACAGAATCGTGACATCATTGGTGTGGCTGAGACTGGCAGTGGCAAGACAGCAG
  CCTTCCTCATCCCTCTGCTGGTCTGGATCACCACACTTCCCAAAATTGACAGGATCGAAGAGTCAGACCA
  AGGCCCTTATGCCATCATCCTGGCTCCCACCCGTGAGTTGGCTCAACAGATTGAGGAAGAGACCATCAAG
  TTTGGGAAACCGCTAGGTATCCGCACTGTGGCTGTCATTGGTGGCATCTCCAGAGAAGACCAGGGCTTCA
  GGCTGCGCATGGGTTGTGAGATTGTGATTGCTACCCCTGGGCGTTTGATTGATGTGCTGGAGAACCGCTA
  CCTGGTGCTGAGCCGCTGTACCTATGTGGTTCTGGATGAGGCAGATAGGATGATTGACATGGGCTTTGAG
  CCAGATGTCCAGAAGATCCTGGAGCACATGCCTGTCAGCAACCAGAAGCCAGACACGGATGAGGCTGAGG
  ACCCTGAGAAGATGCTGGCCAACTTTGAGTCGGGAAAACATAAGTACCGCCAAACAGTCATGTTCACGGC
  CACCATGCCCCCAGCGGTGGAGCGTCTGGCCAGGAGCTATCTTCGGCGACCTGCTGTGGTGTACATTGGC
  TCCGCAGGCAAGCCCCATGAGCGTGTGGAACAGAAGGTCTTCCTCATGTCAGAGTCAGAAAAGAGGAAAA
  AGCTGCTGGCAATCTTGGAGCAAGGCTTTGACCCACCCATCATTATTTTTGTCAACCAGAAGAAGGGCTG
  CGACGTGTTGGCCAAATCCCTGGAGAAGATGGGGTACAATGCTTGCACACTGCACGGTGGAAAAGGCCAG
  GAGCAGCGAGAGTTTGCGTTGTCCAACCTCAAGGCTGGGGCCAAGGATATTTTGGTGGCTACAGATGTGG
  CTGGTCGTGGTATTGACATCCAAGATGTGTCTATGGTTGTCAACTATGATATGGCCAAAAATATTGAAGA
  TTACATCCACCGCATTGGCCGCACGGGACGAGCAGGCAAGAGTGGGGTGGCCATCACCTTCCTCACAAAA
  GAGGACTCTGCTGTGTTCTACGAGCTGAAGCAAGCTATCCTGGAAAGCCCAGTGTCTTCCTGTCCCCCCG
  AACTAGCCAACCACCCAGATGCCCAGCATAAGCCAGGCACCATCCTCACCAAGAAGCGCCGGGAAGAGAC
  CATCTTTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201758 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGELADKKDRDASPSKEERKRSRTPDRERDRDRDRKSSPSKDRKRHRSRDRRRGGSRSRSRSRSKSAER
  ERRHKERERDKERDRNKKDRDRDKDGHRRDKDRKRSSLSPGRGKDFKSRKDRDSKKDEEDEHGDKKPKAQ
  PLSLEELLAKKKAEEEAEAKPKFLSKAEREAEALKRRQQEVEERQRMLEEERKKRKQFQDLGRKMLEDPQ
  ERERRERRERMERETNGNEDEEGRQKIREEKDKSKELHAIKERYLGGIKKRRRTRHLNDRKFVFEWDASE
  DTSIDYNPLYKERHQVQLLGRGFIAGIDLKQQKREQSRFYGDLMEKRRTLEEKEQEEARLRKLRKKEAKQ
  RWDDRHWSQKKLDEMTDRDWRIFREDYSITTKGGKIPNPIRSWKDSSLPPHILEVIDKCGYKEPTPIQRQ
  AIPIGLQNRDIIGVAETGSGKTAAFLIPLLVWITTLPKIDRIEESDQGPYAIILAPTRELAQQIEEETIK
  FGKPLGIRTVAVIGGISREDQGFRLRMGCEIVIATPGRLIDVLENRYLVLSRCTYVVLDEADRMIDMGFE
  PDVQKILEHMPVSNQKPDTDEAEDPEKMLANFESGKHKYRQTVMFTATMPPAVERLARSYLRRPAVVYIG
  SAGKPHERVEQKVFLMSESEKRKKLLAILEQGFDPPIIIFVNQKKGCDVLAKSLEKMGYNACTLHGGKGQ
  EQREFALSNLKAGAKDILVATDVAGRGIDIQDVSMVVNYDMAKNIEDYIHRIGRTGRAGKSGVAITFLTK
  EDSAVFYELKQAILESPVSSCPPELANHPDAQHKPGTILTKKRREETIFA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004818
• ORF Size: 2460 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004818.3
• RefSeq Size: 3288 bp
• RefSeq ORF: 2463 bp
• Locus ID: 9416
• UniProt ID: Q9BUQ8
• Cytogenetics: 12q13.12
• Domains: DEAD, helicase_C
• Protein Pathways: Spliceosome
• MW: 95.6 kDa
• Gene Summary: This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the U5 snRNP complex; it may facilitate conformational changes in the spliceosome during nuclear pre-mRNA splicing. An alternatively spliced transcript variant has been found for this gene, but its biological validity has not been determined. [provided by RefSeq, Jul 2008]