RHOD (NM_014578) Human Tagged ORF Clone

CAT#: RC201722

RHOD (Myc-DDK-tagged)-Human ras homolog gene family, member D (RHOD)

Product Images

Circular map for RC201722

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY RHOD (Cat# RC201722, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RHOD(Cat# TA503891). Positive lysates LY415197 (100ug) and LC415197 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY415197) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201722 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RHOD protein (Cat# TP301722). The protein was produced from HEK293T cells transfected with RHOD cDNA clone (Cat# RC201722) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RHOD
• Synonyms: ARHD; Rho; RHOHP1; RHOM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201722 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGCGGCCCAGGCCGCGGGTGAGGAGGCGCCACCAGGCGTGCGGTCCGTCAAGGTGGTCCTGGTGG
  GCGACGGCGGCTGCGGGAAGACGTCGCTGCTGATGGTCTTCGCCGATGGGGCCTTCCCCGAGAGCTACAC
  CCCCACGGTGTTTGAGCGGTACATGGTCAACCTGCAAGTGAAAGGCAAACCTGTGCACCTCCACATCTGG
  GACACAGCAGGGCAAGATGACTATGACCGCCTGCGGCCCCTGTTCTACCCTGACGCCAGCGTCCTGCTGC
  TTTGCTTCGATGTCACCAGCCCGAACAGCTTTGACAACATCTTTAACCGGTGGTACCCAGAAGTGAATCA
  TTTCTGCAAGAAGGTACCCATCATCGTCGTGGGCTGCAAGACTGACCTGCGCAAGGACAAATCACTGGTG
  AACAAGCTCCGAAGAAACGGATTGGAGCCTGTGACCTACCACAGGGGCCAGGAGATGGCGAGGTCCGTGG
  GCGCGGTGGCCTACCTCGAGTGCTCGGCTCGGCTCCATGACAACGTCCACGCCGTCTTCCAGGAGGCCGC
  CGAGGTGGCCCTCAGCAGCCGCGGTCGCAACTTCTGGCGGCGGATTACCCAGGGCTTTTGCGTGGTGACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201722 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTAAQAAGEEAPPGVRSVKVVLVGDGGCGKTSLLMVFADGAFPESYTPTVFERYMVNLQVKGKPVHLHIW
  DTAGQDDYDRLRPLFYPDASVLLLCFDVTSPNSFDNIFNRWYPEVNHFCKKVPIIVVGCKTDLRKDKSLV
  NKLRRNGLEPVTYHRGQEMARSVGAVAYLECSARLHDNVHAVFQEAAEVALSSRGRNFWRRITQGFCVVT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014578
• ORF Size: 630 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014578.4
• RefSeq Size: 1150 bp
• RefSeq ORF: 633 bp
• Locus ID: 29984
• UniProt ID: O00212
• Cytogenetics: 11q13.2
• Protein Pathways: Axon guidance
• MW: 23.5 kDa
• Gene Summary: Ras homolog, or Rho, proteins interact with protein kinases and may serve as targets for activated GTPase. They play a critical role in muscle differentiation. The protein encoded by this gene binds GTP and is a member of the small GTPase superfamily. It is involved in endosome dynamics and reorganization of the actin cytoskeleton, and it may coordinate membrane transport with the function of the cytoskeleton. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]