Ribophorin I (RPN1) (NM_002950) Human Tagged ORF Clone

CAT#: RC201554

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RPN1 (Myc-DDK-tagged)-Human ribophorin I (RPN1)



  "NM_002950" in other vectors (5)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol RPN1
Synonyms OST1; RBPH1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC201554 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCGCCAGCCGCCGGCTTGTTTCTGCTCCTGTTGCTTGGGACTTGGGCCCCGGCGCCGGGCAGCG
CCTCCTCCGAGGCACCGCCGCTGATCAATGAGGACGTGAAGCGCACAGTGGACCTAAGCAGCCACCTGGC
TAAGGTGACGGCCGAGGTGGTCCTGGCGCACCTGGGCGGCGGCTCCACGTCCCGAGCTACCTCTTTCCTG
CTGGCTTTGGAGCCTGAGCTCGAGGCCCGGCTGGCGCACCTGGGCGTGCAGGTAAAGGGAGAAGATGAGG
AAGAGAACAATTTGGAAGTACGTGAAACCAAAATTAAGGGTAAAAGTGGGAGATTCTTCACAGTCAAGCT
CCCAGTTGCTCTTGATCCTGGGGCCAAGATTTCAGTCATTGTGGAAACAGTCTACACCCATGTGCTTCAT
CCATATCCAACCCAGATCACCCAGTCAGAGAAACAGTTTGTGGTGTTTGAGGGGAACCATTATTTCTACT
CTCCCTATCCAACGAAGACACAAACCATGCGTGTGAAGCTTGCCTCTCGAAATGTGGAGAGCTACACCAA
GCTGGGGAACCCCACGCGCTCTGAGGACCTACTGGATTATGGGCCTTTCAGAGATGTGCCTGCCTATAGT
CAGGATACTTTTAAAGTACATTATGAGAACAACAGCCCTTTCCTGACCATCACCAGCATGACCCGAGTCA
TTGAAGTCTCTCACTGGGGTAATATTGCTGTGGAAGAAAATGTGGACTTAAAGCACACAGGAGCTGTGCT
TAAGGGGCCTTTCTCACGCTATGATTACCAGAGACAGCCAGATAGTGGAATATCCTCCATCCGTTCTTTT
AAGACCATCCTTCCTGCTGCTGCCCAGGATGTTTATTACCGGGATGAGATTGGCAATGTTTCTACCAGCC
ACCTCCTTATTTTGGATGACTCTGTAGAGATGGAAATCCGGCCTCGCTTCCCTCTCTTTGGCGGGTGGAA
GACCCATTACATCGTTGGCTACAACCTCCCAAGCTATGAGTACCTCTATAATTTGGGTGACCAGTATGCA
CTGAAGATGAGGTTTGTGGACCATGTGTTTGATGAACAAGTGATAGATTCTCTGACTGTGAAGATCATCC
TGCCTGAAGGAGCCAAGAACATTGAAATTGATAGTCCCTATGAAATCAGCCGTGCCCCAGATGAGCTGCA
CTACACCTATCTGGATACATTTGGCCGCCCTGTGATTGTTGCCTACAAGAAAAATCTGGTAGAACAGCAC
ATTCAGGACATTGTGGTCCACTACACGTTCAACAAGGTGCTCATGCTGCAGGAGCCCCTGCTGGTGGTGG
CGGCCTTCTACATCCTGTTCTTCACCGTTATCATCTATGTTCGGCTGGACTTCTCCATCACCAAGGATCC
AGCCGCAGAAGCCAGGATGAAGGTAGCCTGCATCACAGAGCAGGTCTTGACCCTGGTCAACAAGAGAATA
GGCCTTTACCGTCACTTTGACGAGACCGTCAATAGGTACAAGCAATCCCGGGACATCTCCACCCTCAACA
GTGGCAAGAAGAGCCTGGAGACTGAACACAAGGCCTTGACCAGTGAGATTGCACTGCTGCAGTCCAGGCT
GAAGACAGAGGGCTCTGATCTGTGCGACAGAGTGAGCGAAATGCAGAAGCTGGATGCACAGGTCAAGGAG
CTGGTGCTGAAGTCGGCGGTGGAGGCTGAGCGCCTGGTGGCTGGCAAGCTCAAGAAAGACACGTACATTG
AGAATGAGAAGCTCATCTCAGGAAAGCGCCAGGAGCTGGTCACCAAGATCGACCACATCCTGGATGCCCT
G


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC201554 protein sequence
Red=Cloning site Green=Tags(s)

MEAPAAGLFLLLLLGTWAPAPGSASSEAPPLINEDVKRTVDLSSHLAKVTAEVVLAHLGGGSTSRATSFL
LALEPELEARLAHLGVQVKGEDEEENNLEVRETKIKGKSGRFFTVKLPVALDPGAKISVIVETVYTHVLH
PYPTQITQSEKQFVVFEGNHYFYSPYPTKTQTMRVKLASRNVESYTKLGNPTRSEDLLDYGPFRDVPAYS
QDTFKVHYENNSPFLTITSMTRVIEVSHWGNIAVEENVDLKHTGAVLKGPFSRYDYQRQPDSGISSIRSF
KTILPAAAQDVYYRDEIGNVSTSHLLILDDSVEMEIRPRFPLFGGWKTHYIVGYNLPSYEYLYNLGDQYA
LKMRFVDHVFDEQVIDSLTVKIILPEGAKNIEIDSPYEISRAPDELHYTYLDTFGRPVIVAYKKNLVEQH
IQDIVVHYTFNKVLMLQEPLLVVAAFYILFFTVIIYVRLDFSITKDPAAEARMKVACITEQVLTLVNKRI
GLYRHFDETVNRYKQSRDISTLNSGKKSLETEHKALTSEIALLQSRLKTEGSDLCDRVSEMQKLDAQVKE
LVLKSAVEAERLVAGKLKKDTYIENEKLISGKRQELVTKIDHILDAL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002950
ORF Size 1821 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002950.2
RefSeq Size 2372 bp
RefSeq ORF 1824 bp
Locus ID 6184
UniProt ID P04843
Cytogenetics 3q21.3
Domains Ribophorin_I
Protein Families Transmembrane
Protein Pathways Metabolic pathways, N-Glycan biosynthesis
MW 68.6 kDa
Gene Summary This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. [provided by RefSeq, Jul 2008]

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