Ribophorin I (RPN1) (NM_002950) Human Tagged ORF Clone

CAT#: RC201554

RPN1 (Myc-DDK-tagged)-Human ribophorin I (RPN1)

Product Images

Circular map for RC201554

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY RPN1 (Cat# RC201554, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPN1(Cat# TA503649). Positive lysates LY418997 (100ug) and LC418997 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY418997) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201554 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RPN1 protein (Cat# TP301554). The protein was produced from HEK293T cells transfected with RPN1 cDNA clone (Cat# RC201554) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ribophorin I
• Synonyms: OST1; RBPH1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201554 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCGCCAGCCGCCGGCTTGTTTCTGCTCCTGTTGCTTGGGACTTGGGCCCCGGCGCCGGGCAGCG
  CCTCCTCCGAGGCACCGCCGCTGATCAATGAGGACGTGAAGCGCACAGTGGACCTAAGCAGCCACCTGGC
  TAAGGTGACGGCCGAGGTGGTCCTGGCGCACCTGGGCGGCGGCTCCACGTCCCGAGCTACCTCTTTCCTG
  CTGGCTTTGGAGCCTGAGCTCGAGGCCCGGCTGGCGCACCTGGGCGTGCAGGTAAAGGGAGAAGATGAGG
  AAGAGAACAATTTGGAAGTACGTGAAACCAAAATTAAGGGTAAAAGTGGGAGATTCTTCACAGTCAAGCT
  CCCAGTTGCTCTTGATCCTGGGGCCAAGATTTCAGTCATTGTGGAAACAGTCTACACCCATGTGCTTCAT
  CCATATCCAACCCAGATCACCCAGTCAGAGAAACAGTTTGTGGTGTTTGAGGGGAACCATTATTTCTACT
  CTCCCTATCCAACGAAGACACAAACCATGCGTGTGAAGCTTGCCTCTCGAAATGTGGAGAGCTACACCAA
  GCTGGGGAACCCCACGCGCTCTGAGGACCTACTGGATTATGGGCCTTTCAGAGATGTGCCTGCCTATAGT
  CAGGATACTTTTAAAGTACATTATGAGAACAACAGCCCTTTCCTGACCATCACCAGCATGACCCGAGTCA
  TTGAAGTCTCTCACTGGGGTAATATTGCTGTGGAAGAAAATGTGGACTTAAAGCACACAGGAGCTGTGCT
  TAAGGGGCCTTTCTCACGCTATGATTACCAGAGACAGCCAGATAGTGGAATATCCTCCATCCGTTCTTTT
  AAGACCATCCTTCCTGCTGCTGCCCAGGATGTTTATTACCGGGATGAGATTGGCAATGTTTCTACCAGCC
  ACCTCCTTATTTTGGATGACTCTGTAGAGATGGAAATCCGGCCTCGCTTCCCTCTCTTTGGCGGGTGGAA
  GACCCATTACATCGTTGGCTACAACCTCCCAAGCTATGAGTACCTCTATAATTTGGGTGACCAGTATGCA
  CTGAAGATGAGGTTTGTGGACCATGTGTTTGATGAACAAGTGATAGATTCTCTGACTGTGAAGATCATCC
  TGCCTGAAGGAGCCAAGAACATTGAAATTGATAGTCCCTATGAAATCAGCCGTGCCCCAGATGAGCTGCA
  CTACACCTATCTGGATACATTTGGCCGCCCTGTGATTGTTGCCTACAAGAAAAATCTGGTAGAACAGCAC
  ATTCAGGACATTGTGGTCCACTACACGTTCAACAAGGTGCTCATGCTGCAGGAGCCCCTGCTGGTGGTGG
  CGGCCTTCTACATCCTGTTCTTCACCGTTATCATCTATGTTCGGCTGGACTTCTCCATCACCAAGGATCC
  AGCCGCAGAAGCCAGGATGAAGGTAGCCTGCATCACAGAGCAGGTCTTGACCCTGGTCAACAAGAGAATA
  GGCCTTTACCGTCACTTTGACGAGACCGTCAATAGGTACAAGCAATCCCGGGACATCTCCACCCTCAACA
  GTGGCAAGAAGAGCCTGGAGACTGAACACAAGGCCTTGACCAGTGAGATTGCACTGCTGCAGTCCAGGCT
  GAAGACAGAGGGCTCTGATCTGTGCGACAGAGTGAGCGAAATGCAGAAGCTGGATGCACAGGTCAAGGAG
  CTGGTGCTGAAGTCGGCGGTGGAGGCTGAGCGCCTGGTGGCTGGCAAGCTCAAGAAAGACACGTACATTG
  AGAATGAGAAGCTCATCTCAGGAAAGCGCCAGGAGCTGGTCACCAAGATCGACCACATCCTGGATGCCCT
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201554 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEAPAAGLFLLLLLGTWAPAPGSASSEAPPLINEDVKRTVDLSSHLAKVTAEVVLAHLGGGSTSRATSFL
  LALEPELEARLAHLGVQVKGEDEEENNLEVRETKIKGKSGRFFTVKLPVALDPGAKISVIVETVYTHVLH
  PYPTQITQSEKQFVVFEGNHYFYSPYPTKTQTMRVKLASRNVESYTKLGNPTRSEDLLDYGPFRDVPAYS
  QDTFKVHYENNSPFLTITSMTRVIEVSHWGNIAVEENVDLKHTGAVLKGPFSRYDYQRQPDSGISSIRSF
  KTILPAAAQDVYYRDEIGNVSTSHLLILDDSVEMEIRPRFPLFGGWKTHYIVGYNLPSYEYLYNLGDQYA
  LKMRFVDHVFDEQVIDSLTVKIILPEGAKNIEIDSPYEISRAPDELHYTYLDTFGRPVIVAYKKNLVEQH
  IQDIVVHYTFNKVLMLQEPLLVVAAFYILFFTVIIYVRLDFSITKDPAAEARMKVACITEQVLTLVNKRI
  GLYRHFDETVNRYKQSRDISTLNSGKKSLETEHKALTSEIALLQSRLKTEGSDLCDRVSEMQKLDAQVKE
  LVLKSAVEAERLVAGKLKKDTYIENEKLISGKRQELVTKIDHILDAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002950
• ORF Size: 1821 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002950.2
• RefSeq Size: 2372 bp
• RefSeq ORF: 1824 bp
• Locus ID: 6184
• UniProt ID: P04843
• Cytogenetics: 3q21.3
• Domains: Ribophorin_I
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• MW: 68.6 kDa
• Gene Summary: This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. [provided by RefSeq, Jul 2008]