DPH2 (NM_001384) Human Tagged ORF Clone

CAT#: RC201382

DPH2 (Myc-DDK-tagged)-Human DPH2 homolog (S. cerevisiae) (DPH2), transcript variant 1

Product Images

Circular map for RC201382

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DPH2 (Cat# RC201382, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DPH2(Cat# TA504855). Positive lysates LY419990 (100ug) and LC419990 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY419990) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201382 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified DPH2 protein (Cat# TP301382). The protein was produced from HEK293T cells transfected with DPH2 cDNA clone (Cat# RC201382) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DPH2
• Synonyms: DPH2L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201382 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCGATGTTTAGCAGCCCTGCCGAGGCGGCGCTGCAGCGAGAGACCGGGGTGCCAGGACTGCTTA
  CTCCTCTTCCGGACCTGGACGGAGTGTACGAGCTGGAGCGAGTCGCTGGATTTGTCCGCGACCTGGGGTG
  TGAACGAGTTGCCTTGCAGTTCCCTGACCAGCTATTGGGAGATGCTGTGGCTGTGGCTGCACGACTGGAG
  GAGACGACAGGGTCAAAGATGTTCATTCTGGGTGACACAGCCTACGGCAGCTGCTGCGTGGATGTGCTGG
  GTGCTGAGCAAGCTGGAGCTCAGGCTCTCATACATTTTGGCCCTGCCTGCTTAAGCCCTCCAGCCCGCCC
  ACTGCCCGTTGCCTTCGTGCTTCGTCAACGTTCTGTGGCCTTGGAGCTCTGTGTCAAGGCCTTTGAGGCC
  CAGAACCCAGACCCCAAAGCGCCTGTGGTGCTGCTGAGTGAGCCGGCCTGTGCCCATGCCCTGGAGGCTT
  TGGCTACTCTCCTGCGCCCACGGTACCTGGACCTGCTAGTCTCCAGCCCAGCTTTTCCCCAACCAGTGGG
  TTCCCTGAGTCCAGAGCCTATGCCCCTAGAGCGTTTTGGGCGCCGCTTCCCCCTTGCCCCAGGGAGGCGT
  CTAGAAGAGTATGGTGCCTTCTATGTAGGGGGCTCTAAGGCCAGCCCTGACCCAGACCTTGACCCAGACC
  TGAGTCGGCTGCTCTTGGGGTGGGCACCAGGTCAACCCTTCTCCTCCTGCTGTCCAGATACAGGGAAGAC
  TCAGGATGAGGGTGCCCGGGCTGGACGGCTAAGGGCACGAAGACGATATCTGGTAGAGAGGGCCAGAGAT
  GCCCGCGTGGTAGGGCTGCTGGCAGGCACACTGGGTGTAGCCCAACACCGTGAGGCACTGGCCCACTTGC
  GGAACCTGACTCAGGCTGCTGGCAAGCGTAGCTATGTGTTGGCCCTGGGGCGGCCCACCCCTGCCAAGCT
  TGCCAACTTCCCTGAGGTGGATGTCTTTGTGCTATTAGCCTGTCCTCTGGGTGCTCTAGCCCCCCAGCTT
  TCTGGTAGCTTCTTCCAGCCTATACTGGCACCATGTGAGCTGGAAGCTGCCTGCAACCCTGCCTGGCCAC
  CTCCAGGCCTGGCTCCCCACCTCACACATTATGCGGACTTATTGCCTGGCTCTCCCTTCCACGTGGCTCT
  CCCACCACCTGAGTCAGAGCTGTGGGAAACCCCAGACGTGTCACTCATTACTGGAGATCTCCGACCCCCA
  CCTGCCTGGAAGTCATCAAATGATCATGGAAGCTTGGCTCTGACCCCACGGCCCCAGCTGGAGCTGGCTG
  AGAGCAGTCCTGCAGCCTCATTCCTTAGTTCCCGGAGCTGGCAAGGGCTGGAGCCCCGCCTGGGTCAGAC
  GCCAGTGACAGAAGCTGTGAGTGGAAGACGAGGGATTGCCATCGCCTATGAGGATGAGGGAAGCGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201382 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MESMFSSPAEAALQRETGVPGLLTPLPDLDGVYELERVAGFVRDLGCERVALQFPDQLLGDAVAVAARLE
  ETTGSKMFILGDTAYGSCCVDVLGAEQAGAQALIHFGPACLSPPARPLPVAFVLRQRSVALELCVKAFEA
  QNPDPKAPVVLLSEPACAHALEALATLLRPRYLDLLVSSPAFPQPVGSLSPEPMPLERFGRRFPLAPGRR
  LEEYGAFYVGGSKASPDPDLDPDLSRLLLGWAPGQPFSSCCPDTGKTQDEGARAGRLRARRRYLVERARD
  ARVVGLLAGTLGVAQHREALAHLRNLTQAAGKRSYVLALGRPTPAKLANFPEVDVFVLLACPLGALAPQL
  SGSFFQPILAPCELEAACNPAWPPPGLAPHLTHYADLLPGSPFHVALPPPESELWETPDVSLITGDLRPP
  PAWKSSNDHGSLALTPRPQLELAESSPAASFLSSRSWQGLEPRLGQTPVTEAVSGRRGIAIAYEDEGSG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001384
• ORF Size: 1467 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001384.5
• RefSeq Size: 2513 bp
• RefSeq ORF: 1470 bp
• Locus ID: 1802
• UniProt ID: Q9BQC3
• Cytogenetics: 1p34.1
• Domains: Diphthamide_syn
• MW: 52.1 kDa
• Gene Summary: This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]