Cyclin (CCNI) (NM_006835) Human Tagged ORF Clone

CAT#: RC201186

CCNI (Myc-DDK-tagged)-Human cyclin I (CCNI)

Product Images

Circular map for RC201186

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CCNI (Cat# RC201186, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCNI (Cat# TA811170)(1:500). Positive lysates LY416390 (100ug) and LC416390 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY416390) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201186 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CCNI protein (Cat# TP301186). The protein was produced from HEK293T cells transfected with CCNI cDNA clone (Cat# RC201186) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cyclin
• Synonyms: CCNI1; CYC1; CYI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201186 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTTTCCAGGGCCTTTGGAAAACCAGAGATTGTCTTTCCTGTTGGAAAAGGCAATCACTAGGGAAG
  CACAGATGTGGAAAGTGAATGTGCGGAAAATGCCTTCAAATCAGAATGTTTCTCCATCCCAGAGAGATGA
  AGTAATTCAATGGCTGGCCAAACTCAAGTACCAATTCAACCTTTACCCAGAAACATTTGCTCTGGCTAGC
  AGTCTTTTGGATAGGTTTTTAGCTACCGTAAAGGCTCATCCAAAATACTTGAGTTGTATTGCAATCAGCT
  GTTTTTTCCTAGCTGCCAAGACTGTTGAGGAAGATGAGAGAATTCCAGTACTAAAGGTATTGGCAAGAGA
  CAGTTTCTGTGGATGTTCCTCATCTGAAATTTTGAGAATGGAGAGAATTATTCTGGATAAGTTGAATTGG
  GATCTTCACACAGCCACACCATTGGATTTTCTTCATATTTTCCATGCCATTGCAGTGTCAACTAGGCCTC
  AGTTACTTTTCAGTTTGCCCAAATTGAGCCCATCTCAACATTTGGCAGTCCTTACCAAGCAACTACTTCA
  CTGTATGGCCTGCAACCAACTTCTGCAATTCAGAGGATCCATGCTTGCTCTGGCCATGGTTAGTCTGGAA
  ATGGAGAAACTCATTCCTGATTGGCTTTCTCTTACAATTGAACTGCTTCAGAAAGCACAGATGGATAGCT
  CCCAGTTGATCCATTGTCGGGAGCTTGTGGCACATCACCTTTCTACTCTGCAGTCTTCCCTGCCTCTGAA
  TTCCGTTTATGTCTACCGTCCCCTCAAGCACACCCTGGTGACCTGTGACAAAGGAGTGTTCAGATTACAT
  CCCTCCTCTGTCCCAGGCCCAGACTTCTCCAAGGACAACAGCAAGCCAGAAGTGCCAGTCAGAGGTACAG
  CAGCCTTTTACCATCATCTCCCAGCTGCCAGTGGGTGCAAGCAGACCTCTACTAAACGCAAAGTAGAGGA
  AATGGAAGTGGATGACTTCTATGATGGAATCAAACGGCTCTATAATGAAGATAATGTCTCAGAAAATGTG
  GGTTCTGTGTGTGGCACTGATTTATCAAGACAAGAGGGACATGCTTCCCCTTGTCCACCTTTGCAGCCTG
  TTTCTGTCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201186 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKFPGPLENQRLSFLLEKAITREAQMWKVNVRKMPSNQNVSPSQRDEVIQWLAKLKYQFNLYPETFALAS
  SLLDRFLATVKAHPKYLSCIAISCFFLAAKTVEEDERIPVLKVLARDSFCGCSSSEILRMERIILDKLNW
  DLHTATPLDFLHIFHAIAVSTRPQLLFSLPKLSPSQHLAVLTKQLLHCMACNQLLQFRGSMLALAMVSLE
  MEKLIPDWLSLTIELLQKAQMDSSQLIHCRELVAHHLSTLQSSLPLNSVYVYRPLKHTLVTCDKGVFRLH
  PSSVPGPDFSKDNSKPEVPVRGTAAFYHHLPAASGCKQTSTKRKVEEMEVDDFYDGIKRLYNEDNVSENV
  GSVCGTDLSRQEGHASPCPPLQPVSVM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006835
• ORF Size: 1131 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006835.3
• RefSeq Size: 1890 bp
• RefSeq ORF: 1134 bp
• Locus ID: 10983
• UniProt ID: Q14094
• Cytogenetics: 4q21.1
• Domains: CYCLIN, cyclin
• MW: 42.6 kDa
• Gene Summary: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin shows the highest similarity with cyclin G. The transcript of this gene was found to be expressed constantly during cell cycle progression. [provided by RefSeq, Jan 2017]