ALDH3B2 (NM_001031615) Human Tagged ORF Clone

CAT#: RC201125

ALDH3B2 (Myc-DDK-tagged)-Human aldehyde dehydrogenase 3 family, member B2 (ALDH3B2), transcript variant 2

Product Images

Circular map for RC201125

• 

  Western blot validation of overexpression lysate (Cat# LY424565) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221866 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ALDH3B2
• Synonyms: ALDH8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201125 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGAAGGATGAACCACGGTCCACGAACCTGTTCATGAAGCTGGACTCGGTCTTCATCTGGAAGGAACCCT
  TTGGCCTGGTCCTCATCATCGCACCCTGGAACTACCCACTGAACCTGACCCTGGTGCTCCTGGTGGGCGC
  CCTCGCCGCAGGGAGTTGCGTGGTGCTGAAGCCGTCAGAAATCAGCCAGGGCACAGAGAAGGTCCTGGCT
  GAGGTGCTGCCCCAGTACCTGGACCAGAGCTGCTTTGCCGTGGTGCTGGGCGGACCCCAGGAGACAGGGC
  AGCTGCTAGAGCACAAGTTGGACTACATCTTCTTCACAGGGAGCCCTCGTGTGGGCAAGATTGTCATGAC
  TGCTGCCACCAAGCACCTGACGCCTGTCACCCTGGAGCTGGGGGGCAAGAACCCCTGCTACGTGGACGAC
  AACTGCGACCCCCAGACCGTGGCCAACCGCGTGGCCTGGTTCTGCTACTTCAATGCCGGCCAGACCTGCG
  TGGCCCCTGACTACGTCCTGTGCAGCCCCGAGATGCAGGAGAGGCTGCTGCCCGCCCTGCAGAGCACCAT
  CACCCGTTTCTATGGCGACGACCCCCAGAGCTCCCCAAACCTGGGCCGCATCATCAACCAGAAACAGTTC
  CAGCGGCTGCGGGCATTGCTGGGCTGCGGCCGCGTGGCCATTGGGGGCCAGAGCAACGAGAGCGATCGCT
  ACATCGCCCCCACGGTGCTGGTGGACGTGCAGGAGACGGAGCCTGTGATGCAGGAGGAGATCTTCGGGCC
  CATCCTGCCCATCGTGAACGTGCAGAGCGTGGACGAGGCCATCAAGTTCATCAACTGGCAGGAGAAGCCC
  CTGGCCCTGTACGCCTTCTCCAACAGCAGCCAGGTTGTGAACCAGATGCTGGAGCGGACCAGCAGCGGCA
  GCTTTGGAGGCAATGAGGGCTTCACCTACATATCTCTGCTGTCCGTGCCATTCGGGGGAGTCGGCCACAG
  TGGGATGGGCCGGTACCACGGCAAGTTCACCTTCGACACCTTCTCCCACCACCGCACCTGCCTGCTCGCC
  CCCTCCGGCCTGGAGAAATTAAAGGAGATCCACTACCCACCCTATACCGACTGGAACCAGCAGCTGTTAC
  GCTGGGGCATGGGCTCCCAGAGCTGTACCCTCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201125 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKDEPRSTNLFMKLDSVFIWKEPFGLVLIIAPWNYPLNLTLVLLVGALAAGSCVVLKPSEISQGTEKVLA
  EVLPQYLDQSCFAVVLGGPQETGQLLEHKLDYIFFTGSPRVGKIVMTAATKHLTPVTLELGGKNPCYVDD
  NCDPQTVANRVAWFCYFNAGQTCVAPDYVLCSPEMQERLLPALQSTITRFYGDDPQSSPNLGRIINQKQF
  QRLRALLGCGRVAIGGQSNESDRYIAPTVLVDVQETEPVMQEEIFGPILPIVNVQSVDEAIKFINWQEKP
  LALYAFSNSSQVVNQMLERTSSGSFGGNEGFTYISLLSVPFGGVGHSGMGRYHGKFTFDTFSHHRTCLLA
  PSGLEKLKEIHYPPYTDWNQQLLRWGMGSQSCTLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001031615
• ORF Size: 1155 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001031615.1, NP_001026786.1
• RefSeq Size: 2504 bp
• RefSeq ORF: 1158 bp
• Locus ID: 222
• Cytogenetics: 11q13.2
• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - cytochrome P450, Glycolysis / Gluconeogenesis, Histidine metabolism, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Phenylalanine metabolism, Tyrosine metabolism
• MW: 42.7 kDa
• Gene Summary: This gene encodes a member of the aldehyde dehydrogenase family, a group of isozymes that may play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The gene of this particular family member is over 10 kb in length. Altered methylation patterns at this locus have been observed in spermatozoa derived from patients exhibiting reduced fecundity. [provided by RefSeq, Aug 2017]