ECM1 (NM_004425) Human Tagged ORF Clone

CAT#: RC201118

ECM1 (Myc-DDK-tagged)-Human extracellular matrix protein 1 (ECM1), transcript variant 1

Product Images

Circular map for RC201118

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  Western blot validation of overexpression lysate (Cat# LY417996) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201118 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ECM1 protein (Cat# TP301118). The protein was produced from HEK293T cells transfected with ECM1 cDNA clone (Cat# RC201118) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ECM1
• Synonyms: URBWD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201118 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGACCACAGCCAGAGCAGCCTTGGTCTTGACCTATTTGGCTGTTGCTTCTGCTGCCTCTGAGGGAG
  GCTTCACGGCTACAGGACAGAGGCAGCTGAGGCCAGAGCACTTTCAAGAAGTTGGCTACGCAGCTCCCCC
  CTCCCCACCCCTATCCCGAAGCCTCCCCATGGATCACCCTGACTCCTCTCAGCATGGCCCTCCCTTTGAG
  GGACAGAGTCAAGTGCAGCCCCCTCCCTCTCAGGAGGCCACCCCTCTCCAACAGGAAAAGCTGCTACCTG
  CCCAACTCCCTGCTGAAAAGGAAGTGGGTCCCCCTCTCCCTCAGGAAGCTGTCCCCCTCCAAAAAGAGCT
  GCCCTCTCTCCAGCACCCCAATGAACAGAAGGAAGGAATGCCAGCTCCATTTGGGGACCAGAGCCATCCA
  GAACCTGAGTCCTGGAATGCAGCCCAGCACTGCCAACAGGACCGGTCCCAAGGGGGCTGGGGCCACCGGC
  TGGATGGCTTCCCCCCTGGGCGGCCTTCTCCAGACAATCTGAACCAAATCTGCCTTCCTAACCGTCAGCA
  TGTGGTATATGGTCCCTGGAACCTACCACAGTCCAGCTACTCCCACCTCACTCGCCAGGGTGAGACCCTC
  AATTTCCTGGAGATTGGATATTCCCGCTGCTGCCACTGCCGCAGCCACACAAACCGCCTAGAGTGTGCCA
  AACTTGTGTGGGAGGAAGCAATGAGCCGATTCTGTGAGGCCGAGTTCTCGGTCAAGACCCGACCCCACTG
  GTGCTGCACGCGGCAGGGGGAGGCTCGGTTCTCCTGCTTCCAGGAGGAAGCTCCCCAGCCACACTACCAG
  CTCCGGGCCTGCCCCAGCCATCAGCCTGATATTTCCTCGGGTCTTGAGCTGCCTTTCCCTCCTGGGGTGC
  CCACATTGGACAATATCAAGAACATCTGCCACCTGAGGCGCTTCCGCTCTGTGCCACGCAACCTGCCAGC
  TACTGACCCCCTACAAAGGGAGCTGCTGGCACTGATCCAGCTGGAGAGGGAGTTCCAGCGCTGCTGCCGC
  CAGGGGAACAATCACACCTGTACATGGAAGGCCTGGGAGGATACCCTTGACAAATACTGTGACCGGGAGT
  ATGCTGTGAAGACCCACCACCACTTGTGTTGCCGCCACCCTCCCAGCCCTACTCGGGATGAGTGCTTTGC
  CCGTCGGGCTCCTTACCCCAACTATGACCGGGACATCTTGACCATTGACATCAGTCGAGTCACCCCCAAC
  CTCATGGGCCACCTCTGTGGAAACCAAAGAGTTCTCACCAAGCATAAACATATTCCTGGGCTGATCCACA
  ACATGACTGCCCGCTGCTGTGACCTGCCATTTCCAGAACAGGCCTGCTGTGCAGAGGAGGAGAAATTAAC
  CTTCATCAATGATCTGTGTGGTCCCCGACGTAACATCTGGCGAGACCCTGCCCTCTGCTGTTACCTGAGT
  CCTGGGGATGAACAGGTCAACTGCTTCAACATCAATTATCTGAGGAACGTGGCTCTAGTGTCTGGAGACA
  CTGAGAACGCCAAGGGCCAGGGGGAGCAGGGCTCAACTGGAGGAACAAATATCAGCTCCACCTCTGAGCC
  CAAGGAAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201118 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGTTARAALVLTYLAVASAASEGGFTATGQRQLRPEHFQEVGYAAPPSPPLSRSLPMDHPDSSQHGPPFE
  GQSQVQPPPSQEATPLQQEKLLPAQLPAEKEVGPPLPQEAVPLQKELPSLQHPNEQKEGMPAPFGDQSHP
  EPESWNAAQHCQQDRSQGGWGHRLDGFPPGRPSPDNLNQICLPNRQHVVYGPWNLPQSSYSHLTRQGETL
  NFLEIGYSRCCHCRSHTNRLECAKLVWEEAMSRFCEAEFSVKTRPHWCCTRQGEARFSCFQEEAPQPHYQ
  LRACPSHQPDISSGLELPFPPGVPTLDNIKNICHLRRFRSVPRNLPATDPLQRELLALIQLEREFQRCCR
  QGNNHTCTWKAWEDTLDKYCDREYAVKTHHHLCCRHPPSPTRDECFARRAPYPNYDRDILTIDISRVTPN
  LMGHLCGNQRVLTKHKHIPGLIHNMTARCCDLPFPEQACCAEEEKLTFINDLCGPRRNIWRDPALCCYLS
  PGDEQVNCFNINYLRNVALVSGDTENAKGQGEQGSTGGTNISSTSEPKEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004425
• ORF Size: 1620 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004425.4
• RefSeq Size: 2161 bp
• RefSeq ORF: 1623 bp
• Locus ID: 1893
• UniProt ID: Q16610
• Cytogenetics: 1q21.2
• Protein Families: Secreted Protein, Transmembrane
• MW: 60.7 kDa
• Gene Summary: This gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. Alternatively spliced transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Feb 2011]