ALG3 (NM_005787) Human Tagged ORF Clone

CAT#: RC201101

ALG3 (Myc-DDK-tagged)-Human asparagine-linked glycosylation 3, alpha-1,3- mannosyltransferase homolog (S. cerevisiae) (ALG3), transcript variant 1

Product Images

Circular map for RC201101

• 

  Western blot validation of overexpression lysate (Cat# LY417074) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201101 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ALG3
• Synonyms: CDG1D; CDGS4; CDGS6; D16Ertd36e; not; Not56; NOT56L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201101 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTGGGCTGCGGAAACGCGGCCGGTCCGGTTCCGCGGCCCAGGCAGAGGGACTCTGCAAGCAAT
  GGCTGCAGCGCGCCTGGCAAGAGCGGCGCCTGCTGCTGCGGGAGCCGCGCTACACGCTGCTGGTGGCCGC
  CTGCCTCTGCCTGGCGGAGGTGGGCATCACCTTCTGGGTCATTCACAGGGTGGCATACACAGAGATTGAC
  TGGAAGGCCTACATGGCCGAGGTAGAAGGCGTCATCAATGGTACCTATGACTATACCCAACTGCAGGGTG
  ACACCGGACCACTTGTGTACCCAGCTGGTTTCGTGTACATCTTTATGGGGTTGTACTATGCCACCAGCCG
  AGGCACTGACATCCGCATGGCCCAGAACATCTTTGCTGTGCTCTACCTGGCTACCTTGCTGCTTGTCTTC
  TTGATCTATCACCAGACCTGCAAGGTACCTCCCTTCGTCTTTTTCTTCATGTGCTGCGCCTCTTACCGTG
  TCCACTCCATCTTTGTGCTGCGGCTCTTCAATGACCCAGTGGCCATGGTGCTGCTCTTCCTCAGTATCAA
  CCTCCTGCTGGCCCAGCGCTGGGGCTGGGGTTGCTGCTTTTTCAGCCTGGCAGTCTCTGTGAAGATGAAT
  GTGCTGCTCTTCGCCCCTGGGTTACTGTTTCTTCTCCTCACACAGTTTGGCTTCCGTGGGGCCCTCCCCA
  AGCTGGGAATCTGTGCTGGCCTTCAGGTGGTGCTGGGGCTGCCCTTCCTGCTGGAGAACCCCAGCGGCTA
  CCTGTCCCGCTCCTTTGACCTTGGCCGCCAGTTTCTGTTCCACTGGACAGTGAACTGGCGCTTCCTCCCA
  GAGGCGCTCTTCCTGCATCGAGCCTTCCACCTGGCCCTGTTGACTGCCCACCTCACCCTGCTCCTGCTGT
  TTGCCCTCTGCAGGTGGCACAGGACAGGGGAAAGTATCTTGTCGCTGCTGAGGGATCCCTCCAAAAGGAA
  GGTTCCACCCCAGCCCCTTACACCCAACCAGATCGTTTCTACCCTCTTCACCTCCAACTTCATTGGCATC
  TGCTTCAGCCGCTCCCTCCACTACCAGTTCTACGTCTGGTATTTCCACACACTGCCCTACCTCCTGTGGG
  CCATGCCTGCACGCTGGCTCACACACCTGCTCAGGTTGTTGGTGCTGGGGCTCATCGAGCTCTCCTGGAA
  CACATACCCTTCCACATCCTGCAGCTCTGCTGCCCTGCACATATGCCATGCCGTCATCCTGCTGCAGCTC
  TGGCTGGGCCCGCAGCCTTTCCCCAAGAGCACCCAACACAGCAAGAAAGCCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201101 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAGLRKRGRSGSAAQAEGLCKQWLQRAWQERRLLLREPRYTLLVAACLCLAEVGITFWVIHRVAYTEID
  WKAYMAEVEGVINGTYDYTQLQGDTGPLVYPAGFVYIFMGLYYATSRGTDIRMAQNIFAVLYLATLLLVF
  LIYHQTCKVPPFVFFFMCCASYRVHSIFVLRLFNDPVAMVLLFLSINLLLAQRWGWGCCFFSLAVSVKMN
  VLLFAPGLLFLLLTQFGFRGALPKLGICAGLQVVLGLPFLLENPSGYLSRSFDLGRQFLFHWTVNWRFLP
  EALFLHRAFHLALLTAHLTLLLLFALCRWHRTGESILSLLRDPSKRKVPPQPLTPNQIVSTLFTSNFIGI
  CFSRSLHYQFYVWYFHTLPYLLWAMPARWLTHLLRLLVLGLIELSWNTYPSTSCSSAALHICHAVILLQL
  WLGPQPFPKSTQHSKKAH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005787
• ORF Size: 1314 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005787.6
• RefSeq Size: 1550 bp
• RefSeq ORF: 1317 bp
• Locus ID: 10195
• UniProt ID: Q92685
• Cytogenetics: 3q27.1
• Domains: ALG3
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• MW: 50.1 kDa
• Gene Summary: This gene encodes a member of the ALG3 family. The encoded protein catalyses the addition of the first dol-P-Man derived mannose in an alpha 1,3 linkage to Man5GlcNAc2-PP-Dol. Defects in this gene have been associated with congenital disorder of glycosylation type Id (CDG-Id) characterized by abnormal N-glycosylation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]