KIF20A (NM_005733) Human Tagged ORF Clone

CAT#: RC201100

KIF20A (Myc-DDK-tagged)-Human kinesin family member 20A (KIF20A)

Product Images

Circular map for RC201100

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  Western blot validation of overexpression lysate (Cat# LY401748) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201100 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified KIF20A protein (Cat# TP301100). The protein was produced from HEK293T cells transfected with KIF20A cDNA clone (Cat# RC201100) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KIF20A
• Synonyms: MKLP2; RAB6KIFL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201100 representing NM_005733
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCAAGGGATCCTTTCTCCGCCAGCGGGCTTGCTGTCCGATGACGATGTCGTAGTTTCTCCCATGT
  TTGAGTCCACAGCTGCAGATTTGGGGTCTGTGGTACGCAAGAACCTGCTATCAGACTGCTCTGTCGTCTC
  TACCTCCCTAGAGGACAAGCAGCAGGTTCCATCTGAGGACAGTATGGAGAAGGTGAAAGTATACTTGAGG
  GTTAGGCCCTTGTTACCTTCAGAGTTGGAACGACAGGAAGATCAGGGTTGTGTCCGTATTGAGAATGTGG
  AGACCCTTGTTCTACAAGCACCCAAGGACTCTTTTGCCCTGAAGAGCAATGAACGGGGAATTGGCCAAGC
  CACACACAGGTTCACCTTTTCCCAGATCTTTGGGCCAGAAGTGGGACAGGCATCCTTCTTCAACCTAACT
  GTGAAGGAGATGGTAAAGGATGTACTCAAAGGGCAGAACTGGCTCATCTATACATATGGAGTCACTAACT
  CAGGGAAAACCCACACGATTCAAGGTACCATCAAGGATGGAGGGATTCTCCCCCGGTCCCTGGCGCTGAT
  CTTCAATAGCCTCCAAGGCCAACTTCATCCAACACCTGATCTGAAGCCCTTGCTCTCCAATGAGGTAATC
  TGGCTAGACAGCAAGCAGATCCGACAGGAGGAAATGAAGAAGCTGTCCCTGCTAAATGGAGGCCTCCAAG
  AGGAGGAGCTGTCCACTTCCTTGAAGAGGAGTGTCTACATCGAAAGTCGGATAGGTACCAGCACCAGCTT
  CGACAGTGGCATTGCTGGGCTCTCTTCTATCAGTCAGTGTACCAGCAGTAGCCAGCTGGATGAAACAAGT
  CATCGATGGGCACAGCCAGACACTGCCCCACTACCTGTCCCGGCAAACATTCGCTTCTCCATCTGGATCT
  CATTCTTTGAGATCTACAACGAACTGCTTTATGACCTATTAGAACCGCCTAGCCAACAGCGCAAGAGGCA
  GACTTTGCGGCTATGCGAGGATCAAAATGGCAATCCCTATGTGAAAGATCTCAACTGGATTCATGTGCAA
  GATGCTGAGGAGGCCTGGAAGCTCCTAAAAGTGGGTCGTAAGAACCAGAGCTTTGCCAGCACCCACCTCA
  ACCAGAACTCCAGCCGCAGTCACAGCATCTTCTCAATCAGGATCCTACACCTTCAGGGGGAAGGAGATAT
  AGTCCCCAAGATCAGCGAGCTGTCACTCTGTGATCTGGCTGGCTCAGAGCGCTGCAAAGATCAGAAGAGT
  GGTGAACGGTTGAAGGAAGCAGGAAACATTAACACCTCTCTACACACCCTGGGCCGCTGTATTGCTGCCC
  TTCGTCAAAACCAGCAGAACCGGTCAAAGCAGAACCTGGTTCCCTTCCGTGACAGCAAGTTGACTCGAGT
  GTTCCAAGGTTTCTTCACAGGCCGAGGCCGTTCCTGCATGATTGTCAATGTGAATCCCTGTGCATCTACC
  TATGATGAAACTCTTCATGTGGCCAAGTTCTCAGCCATTGCTAGCCAGCTTGTGCATGCCCCACCTATGC
  AACTGGGATTCCCATCCCTGCACTCGTTCATCAAGGAACATAGTCTTCAGGTATCCCCCAGCTTAGAGAA
  AGGGGCTAAGGCAGACACAGGCCTTGATGATGATATTGAAAATGAAGCTGACATCTCCATGTATGGCAAA
  GAGGAGCTCCTACAAGTTGTGGAAGCCATGAAGACACTGCTTTTGAAGGAACGACAGGAAAAGCTACAGC
  TGGAGATGCATCTCCGAGATGAAATTTGCAATGAGATGGTAGAACAGATGCAACAGCGGGAACAGTGGTG
  CAGTGAACATTTGGACACCCAAAAGGAACTATTGGAGGAAATGTATGAAGAAAAACTAAATATCCTCAAG
  GAGTCACTGACAAGTTTTTACCAAGAAGAGATTCAGGAGCGGGATGAAAAGATTGAAGAGCTAGAAGCTC
  TCTTGCAGGAAGCCAGACAACAGTCAGTGGCCCATCAGCAATCAGGGTCTGAATTGGCCCTACGGCGGTC
  ACAAAGGTTGGCAGCTTCTGCCTCCACCCAGCAGCTTCAGGAGGTTAAAGCTAAATTACAGCAGTGCAAA
  GCAGAGCTAAACTCTACCACTGAAGAGTTGCATAAGTATCAGAAAATGTTAGAACCACCACCCTCAGCCA
  AGCCCTTCACCATTGATGTGGACAAGAAGTTAGAAGAGGGCCAGAAGAATATAAGGCTGTTGCGGACAGA
  GCTTCAGAAACTTGGTGAGTCTCTCCAATCAGCAGAGAGAGCTTGTTGCCACAGCACTGGGGCAGGAAAA
  CTTCGTCAAGCCTTGACCACTTGTGATGACATCTTAATCAAACAGGACCAGACTCTGGCTGAACTGCAGA
  ACAACATGGTGCTAGTGAAACTGGACCTTCGGAAGAAGGCAGCATGTATTGCTGAGCAGTATCATACTGT
  GTTGAAACTCCAAGGCCAGGTTTCTGCCAAAAAGCGCCTTGGTACCAACCAGGAAAATCAGCAACCAAAC
  CAACAACCACCAGGGAAGAAACCATTCCTTCGAAATTTACTTCCCCGAACACCAACCTGCCAAAGCTCAA
  CAGACTGCAGCCCTTATGCCCGGATCCTACGCTCACGGCGTTCCCCTTTACTCAAATCTGGGCCTTTTGG
  CAAAAAGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201100 representing NM_005733
  Red=Cloning site Green=Tags(s)
  
  MSQGILSPPAGLLSDDDVVVSPMFESTAADLGSVVRKNLLSDCSVVSTSLEDKQQVPSEDSMEKVKVYLR
  VRPLLPSELERQEDQGCVRIENVETLVLQAPKDSFALKSNERGIGQATHRFTFSQIFGPEVGQASFFNLT
  VKEMVKDVLKGQNWLIYTYGVTNSGKTHTIQGTIKDGGILPRSLALIFNSLQGQLHPTPDLKPLLSNEVI
  WLDSKQIRQEEMKKLSLLNGGLQEEELSTSLKRSVYIESRIGTSTSFDSGIAGLSSISQCTSSSQLDETS
  HRWAQPDTAPLPVPANIRFSIWISFFEIYNELLYDLLEPPSQQRKRQTLRLCEDQNGNPYVKDLNWIHVQ
  DAEEAWKLLKVGRKNQSFASTHLNQNSSRSHSIFSIRILHLQGEGDIVPKISELSLCDLAGSERCKDQKS
  GERLKEAGNINTSLHTLGRCIAALRQNQQNRSKQNLVPFRDSKLTRVFQGFFTGRGRSCMIVNVNPCAST
  YDETLHVAKFSAIASQLVHAPPMQLGFPSLHSFIKEHSLQVSPSLEKGAKADTGLDDDIENEADISMYGK
  EELLQVVEAMKTLLLKERQEKLQLEMHLRDEICNEMVEQMQQREQWCSEHLDTQKELLEEMYEEKLNILK
  ESLTSFYQEEIQERDEKIEELEALLQEARQQSVAHQQSGSELALRRSQRLAASASTQQLQEVKAKLQQCK
  AELNSTTEELHKYQKMLEPPPSAKPFTIDVDKKLEEGQKNIRLLRTELQKLGESLQSAERACCHSTGAGK
  LRQALTTCDDILIKQDQTLAELQNNMVLVKLDLRKKAACIAEQYHTVLKLQGQVSAKKRLGTNQENQQPN
  QQPPGKKPFLRNLLPRTPTCQSSTDCSPYARILRSRRSPLLKSGPFGKKY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005733
• ORF Size: 2670 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005733.3
• RefSeq Size: 2972 bp
• RefSeq ORF: 2673 bp
• Locus ID: 10112
• UniProt ID: O95235
• Cytogenetics: 5q31.2
• Domains: kinesin
• Protein Families: Druggable Genome
• MW: 100.1 kDa
• Gene Summary: Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.[UniProtKB/Swiss-Prot Function]