Carbonic Anhydrase III (CA3) (NM_005181) Human Tagged ORF Clone

CAT#: RC201066

CA3 (Myc-DDK-tagged)-Human carbonic anhydrase III, muscle specific (CA3)

Product Images

Circular map for RC201066

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  Western blot validation of overexpression lysate (Cat# LY417461) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201066 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CA3 protein (Cat# TP301066). The protein was produced from HEK293T cells transfected with CA3 cDNA clone (Cat# RC201066) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Carbonic Anhydrase III
• Synonyms: CAIII; Car3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201066 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAGGAGTGGGGCTACGCCAGTCACAACGGTCCTGACCACTGGCATGAACTTTTCCCAAATGCCA
  AGGGGGAAAACCAGTCGCCCATTGAGCTGCATACTAAAGACATCAGGCATGACCCTTCTCTGCAGCCATG
  GTCTGTGTCTTATGATGGTGGCTCTGCCAAGACCATCCTGAATAATGGGAAGACCTGCCGAGTTGTATTT
  GATGATACTTATGATAGGTCAATGCTGAGAGGGGGTCCTCTCCCTGGACCCTACCGACTTCGCCAGTTTC
  ATCTTCACTGGGGCTCTTCGGATGATCATGGCTCTGAGCACACCGTGGATGGAGTCAAGTATGCAGCGGA
  GCTTCATTTGGTTCACTGGAACCCGAAGTATAACACTTTTAAAGAAGCCCTGAAGCAGCGCGATGGGATC
  GCTGTGATTGGCATTTTTCTGAAGATAGGACATGAGAATGGCGAGTTCCAGATTTTCCTTGATGCATTGG
  ACAAGATTAAGACAAAGGGCAAGGAGGCGCCCTTCACAAAGTTTGACCCATCCTGCCTGTTCCCGGCATG
  CCGGGACTACTGGACCTACCAGGGCTCATTCACCACGCCGCCCTGCGAGGAATGCATTGTGTGGCTGCTG
  CTGAAGGAGCCCATGACCGTGAGCTCTGACCAGATGGCCAAGCTGCGGAGCCTCCTCTCCAGTGCTGAGA
  ACGAGCCCCCAGTGCCTCTTGTGAGCAACTGGCGACCTCCACAGCCTATCAATAACAGGGTGGTGAGAGC
  TTCCTTCAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201066 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAKEWGYASHNGPDHWHELFPNAKGENQSPIELHTKDIRHDPSLQPWSVSYDGGSAKTILNNGKTCRVVF
  DDTYDRSMLRGGPLPGPYRLRQFHLHWGSSDDHGSEHTVDGVKYAAELHLVHWNPKYNTFKEALKQRDGI
  AVIGIFLKIGHENGEFQIFLDALDKIKTKGKEAPFTKFDPSCLFPACRDYWTYQGSFTTPPCEECIVWLL
  LKEPMTVSSDQMAKLRSLLSSAENEPPVPLVSNWRPPQPINNRVVRASFK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005181
• ORF Size: 780 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005181.4
• RefSeq Size: 1753 bp
• RefSeq ORF: 783 bp
• Locus ID: 761
• UniProt ID: P07451
• Cytogenetics: 8q21.2
• Domains: carb_anhydrase
• Protein Families: Druggable Genome
• Protein Pathways: Nitrogen metabolism
• MW: 29.6 kDa
• Gene Summary: Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns. [provided by RefSeq, Oct 2008]